Tissues, morphologically intact

Peal off the rubber cement, remove the cover slip, stain the slides for 5 min with 1% Eosin, and observe them under the light microscope. An intact tissue morphology will confirm the quality of slides and fixation (see Note 11). 

It is important to proceed further only if tissue retains its morphology intact after proteinase K digestion. Discontinue the experiment if the tissue is distorted. This saves your valuable time and reagents. 

Molecular Networks in Morphologically Intact Cells and Tissue-Challenge for Biology and Drug Development... 

The present paper summarizes a new approach of target selection by addressing molecular networks directly in morphologically intact cells and tissue. 

In situ hybridization is a specialized type of solid-support assay in which morphologically intact tissue, cells, or chromosomes affixed to a glass microscope slide provide the... 

The melt-spun monofilament fibers from this triblock material were achieved at temperatures about 10-40°C above the corresponding melting temperatures of the copolymers. An in vivo implantation study of a triblock copolymer fiber (of 5/5/90 feed ratio) in rat up to 240 days showed that the monofilament fibers were morphologically intact at 120 days but fragmented at 150 days. Complete mass absorption was achieved at 240 days in rats which is consistent with the reported complete mass absorption for pure PDS sutures. The tissue reaction observed was typical... 

In situ hybridization (ISH) consists of the application of hybridization techniques to intact cells which demonstrate genetic information within a morphologic context. This technology takes advantage of the hybridization properties of nucleic acids and offers a distinct technique to directly analyze sequence information in intact tissues. In essence, it combines cytogenetic techniques with molecular biology to probe gene alterations at molecular levels. Development of... 

Recently, a cell culture derived from biopsies of healthy human buccal mucosa has been developed with remarkably similar morphology, membranecoating granule structure and appearance, and lipid composition to intact buccal tissue [102], The barrier nature of this cell culture model is similar to intact buccal mucosa, and so this cell culture may be an alternative model to the... 

TR146 cell culture. With the development of tissue culture techniques, it is anticipated that various cell culture models may be developed with similar morphological and barrier properties to normal intact buccal mucosa. Such models may be very useful in assessing the buccal permeability and metabolism of many compounds. 

Fixation in formalin is suitable because the induced protein-protein and protein-nucleic acid cross-links preserve the tissue efficiently while retaining morphology relatively intact. However, the macromolecular network introduced by formalin significantly reduces the access of FISH probes to target DNA. Consequently, the initial steps in a FISH staining must address suitable breakdown of this network. 

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