Single-chain Fv

Katakura Y, Kumamoto T, Iwai Y, et al. Fluorescence polarization study of a salt bridge between a single-chain Fv and its antigen ribonuclease A. Mol. Immunol. 1997 34 731-734. 

As discussed above, wheat has been used only rarely for molecular farming. Thus far, the only example of a pharmaceutical protein produced in wheat is a single chain Fv antibody, which was expressed using the Ubil promoter and achieved a maximum expression level of 1.5 pg g 1 dry weight [77]. Transgenic wheat producing Aspergillus phytase has also been reported [78]. 

CP coat protein CtxB cholera toxin B subunit scFv single chain Fv antibody fragment TMOF trypsin modulating oostatic factor MAB monoclonal antibody GFP green fluorescent protein CPV Canine parvovirus BHV Bovine herpes virus FMDV Foot and mouth disease virus HCV Hepatitis C virus HRV Human rhino Virus MEV Mink enteritis virus MHV Murine hepatitis virus MV Measles virus RSV Respiratory syncytial virus... 

ZHU, Q ZENG, C HUHALOV, A., YAO, J., TURI, T.G., DANLEY, D., HYNES, T CONG, Y., DIMATTIA, D., KENNEDY, S., DAUMY, G., SCHAEFFER, E., MARASCO, W.A., HUSTON, J.S., Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7, J. Immunol. Methods, 1999, 231, 207-222. 

Brooks, L., Suckling, C.J. and Stimson, W.H. (1996) Constraction of a single-chain Fv from an antibody which catalyses a Diels Alder cycloaddition. Biochem. Soc. Trans., 24, 313S. 

Fischer, R., Schumann, D., Zinunermann, S., Drossard, J., Sack, M., and Schillberg, S. (1999). Expression and characterization of bispecific single-chain Fv fragments produced in transgenic plants. Ear. J. Biochem. 262 810-816. 

McCormick, A.A., Kumangai, M.H., Hanley, K., Turpen, Y.H., Hakim, I., and Grill, L.K. (1999). Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc. Natl. Acad. Sci. U.S.A. 96 703-708. 

Large Scale Canada Vacaville, vaccine Various single chain Fv Viral vectors in... 

To compensate for that weak interaction, researchers have directly linked Vh and Vi by adding peptide sequences that covalently hold them together. The product is caUed a single-chain Fv protein or sFv... 

Cooke, S.P, R.B. Pedley, R. Boden, R.H. Begent, and K.A. Chester, In vivo tumor delivery of a recombinant single chain Fv tumor necrosis factor-alpha fusion protein. Bioconjug Chem, 2002.13(2) 385. 

Canaan-Haden, L., Ayala, M., Femandez-de-Cossio, M.E., Pedroso, I., Rodes, L., Govilondo, J.V. (1995). Purification and application a single-chain Fv antibody fragment specific to hepatitis virus surface antigen. Biotechniques, 19(4), 606-608. 

Single-chain Fv fragments (scFv) consisting of VH and VL regions linked by a flexible peptide... 

Several systems exist for the expression and cloning of antibodies, as either Fab fragments (Fabs) or single-chain Fv fragments (scFvs) on the surface of... 

Kipryanov, S. M, Little, M, Kropshofer, H., Breitling, F., Gotter, S., and Diibel, S. (1996) Affinity enhancement of a recombinant antibody, formation of complexes with multiple valency by a single-chain Fv fragment-core Streptavidin fusion. Protein Eng 9,203—211... 

Milenic, D.E., Yokota, T., Filpula, D.R., Finkelman, M.A., Dodd, S.W., Wood, J.F. et al. (1991) Construction, binding properties, metabolism, and tumor targeting of a single-chain Fv derived from the pancarcinoma monoclonal antibody CC49. Cancer Res., 51, 6363-6371. 

Key Words Antibody (Ab) fragment antigen binding (Fab) fragment variable (Fv) single-chain Fv (scFv) variable part of heavy chain variable part of light chain wild type. 

Biocca, S., Ruberti, F., Tafani, M., Pierandrei-Amaldi, P., and Cattaneo, A. (1995) Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria. Biol Technology 13, 1110-1115. 

Le Gall, F., Kipriyanov, S. M., Moldenhauer, G., and Little, M. (1999) Di-, tri- and tetrameric single chain Fv antibody fragments against human CD19 effect of valency on cell binding. FEBS Lett. 453, 164-168. 

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