Ubil Promoter

Based on initial studies using rice transformants, it was found that the choice of promoters used to express proper foreign protein in cereals is also important. For example, the promoter element that encodes the Ubil gene-encoding uhiquitin, when combined with an intron, elicited higher levels of expression of foreign protein in immature wheat, maize, and rice embryos than the 35S CaMV promoter (Christou, 1997). 

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As discussed above, wheat has been used only rarely for molecular farming. Thus far, the only example of a pharmaceutical protein produced in wheat is a single chain Fv antibody, which was expressed using the Ubil promoter and achieved a maximum expression level of 1.5 pg g 1 dry weight [77]. Transgenic wheat producing Aspergillus phytase has also been reported [78]. 

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