Separations biochemical

By separating biochemicals on the basis of charge, size, and conformation, electrophoresis can provide valuable information, such as purity, identity, and molecular weight. Purity is indicated by the number of stained bands in the electropherogram. One band usually means that only one detectable component is present that is, the sample is homogeneous or electrophoretically... 

A biochemist typically must dissect an organism into separate biochemical constituents in order to carry out the intended studies. For instance, through isolating and purifying one protein from among tens of thousands in the cell, detailed, closely controlled in vitro experiments can be done on this particular study system (or biochemical trait, if you will). This requirement for simplification is absolutely necessary for controlled experimentation, yet is also the source of a major philosophical problem How do we know that the properties we measure in an isolated system resemble those... 

More recent studies have indicated that TD may be the result of complex interactions between dopamine and the cholinergic system, which becomes more active when the suppressive or balancing effect of the dopaminergic system is blocked by the neuroleptics. In addition, the neuroleptics are directly toxic to neurons by means of disrupting a number of separate biochemical pathways (chapter 5). 

About 500 separate biochemical processes occur in one single liver cell ... 

Thrombin catalyzes the formation of the fibrin dot and, at the same time, the activation of platelets. Are fibrin clot formation and platelet activation totally separate biochemical pathways The answer to this question is yes and no. To some extent, fibrin clot formation and platelet activation occur separately and... 

Applications technologies in metal ion, inorganic species, hydrocarbons separations, biochemical and biomedical applications, and fine particles preparation using ELM are reviewed. Commercial applications include the removal of zinc, phenol, and cyanide from wastewaters. Potential applications in wastewater treatment, biochemical processing, rare earth metal extraction, radioactive material removal, and nickel recovery are described. 

Onishi, S., Itoh, S., and Ishida, Y., Assay of free, glycine- and taurine-conjugated bile acids in serum by high-pressure liquid chromatography by using post-column reaction after group separation, Biochem. J. 204, 135-139 (1982). 

Deficiency with separate biochemical effect but exacerbates the same clinical signs as MeHg (different mechanism, same target)... 

DUV Christian Rene de (b. 1917) English-born Belgian biochemist who used differential centrifugation to separate biochemical tissue fragments into layers and discovered the cell organelles (little organs), the lysosomes, and the peroxisomes. For this... 

Immunoaffinity chromatography (lAC). Immunoaflinity chromatography, based upon a highly specific biological interaction such as that between antigen and antibody, is a bioanalytical method for separating biochemical mixtures. 

Pettitt, TR and Wakelam, MJ (1993) Bombesin stimulates distinct time-dependent changes in the sn-l,2-diradylglycerol molecular species profile from Swiss 3T3 fibroblasts as analysed by 3,5-dinitrobenzoyl derivatization and h.p.l.c. separation. Biochemical Journal, 289, 487 95. 

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