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Centrifugation differential

Differential centrifugation is the simplest form of centrifugation A mixed particle population is subjected to centrifugations with [Pg.164]

The first differential centrifugal extractor to be used in industry was the PodbieHiiak extractor which was introduced in the 1950s (209,210) and can be regarded as a perforated-plate column wrapped around a rotor shaft. Rotation creates a centrifugal force which results in a great reduction in the equivalent height and contact time that would be needed in a conventional perforated-plate column. [Pg.77]

Martinez-Marcos A. and Halpem M. (1999a). Differential centrifugal afferents to the anterior and posterior accessory olfactory bulb. Neuroreport 10, 2011-2015. [Pg.227]

Differential centrifugation, 5 531 Differential contacting, 10 760-762 Differential display, 13 354 Differential equations, 11 736 of motion, 11 738-739 Differential interference contrast (DIC), 16 480-483... [Pg.267]

Some of the results obtained by differential centrifugation showed enzyme distribution between different cell fractions which were difficult to interpret. Enzymes like carbamoyl phosphate synthase or isocitrate dehydrogenase were found both in mitochondria and in the soluble fraction of the cell. This led to detailed kinetic studies with purified enzymes which indicated there might be populations of enzymes with slightly different properties (isozymes) catalyzing similar reactions in different compartments or in different cell types. Variations in kinetic behavior appeared to tailor the enzyme appropriately to the particular compartment or cell where the reaction took place. [Pg.150]

Interpretation (1953-1955) of the ultrastructures which, after cell disruption and differential centrifugation, gave rise to microsomes, proved quite difficult. Porter described a vacuolar system with canal-iculi and cistemae which was particularly evident in protein-secreting cells such as the acinar cells of the pancreas. The appearance was... [Pg.153]

The heyday of differential centrifugation and work on isolated cell fractions was short. In the 1940s and 1950s biochemists attracted much critical comment because of their use of disorganized homogenates and then of separated cell fractions. Many metabolic pathways were, however, successfully analyzed with little reference to their normal setting within the cell. [Pg.162]

Mixer-settlers Nonagitated differential Agitated differential Centrifugal... [Pg.301]

As an example for differential centrifugation, this protocol gives the preparation of membranes of the sarcoplasmic Ontracellular) reticulum (SR) of heart muscle. [Pg.165]

Fig. 11. (a) Differential centrifugation (pelleting), where time 1 < time 2 < time 3 < time, (b) Rate zonal separation in a swinging-bucket rotor, where tube 1 represents the density gradient solution, tube 2 the sample plus the gradient, and tube 3 the separation of sample particles under a centrifugal force, where... [Pg.408]

To obtain optimal results, protein should possess minimum 95% purity. Thus, the molecule under investigation should be purified by gel or column separation, dialysis, differential centrifugation, salting out, or HPLC prior to structural analysis. [Pg.110]

A high-speed differential centrifugal fraction for Giardia mitosomes has been generated that has been successfully used to reconstitute Fe-S cluster... [Pg.31]

Insoluble form (inclusion bodies) homogenization, differential centrifugation, wash, dissolution... [Pg.315]

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