Centrifugation biochemical separation

Pierpoint, W.S. Mitochondrial preparations fiom the leaves of tobacco Nicotiana tabacum). 3. GlycoUic oxidase and fumarase activity Biochem. J. 75 (1960) 511-515. Pierpoint, W.S. Mitochondrial preparations from the leaves of tobacco Nicotiana tabacum). 4. Separation of some components by density-gradient centrifuging Biochem. J. 82 (1962) 143-148. 

DUV Christian Rene de (b. 1917) English-born Belgian biochemist who used differential centrifugation to separate biochemical tissue fragments into layers and discovered the cell organelles (little organs), the lysosomes, and the peroxisomes. For this... 

Enzymatically active, partially purified (washed) rubber particles can be isolated such that, when provided with an appropriate APP primer, magnesium ion cofactor, and IPP monomer, rubber is produced in vitro [253-255]. Fresh latex can be separated by centrifugation into three phases. The bottom fraction (20% of the latex) contains membrane-bound organelles. The middle fraction is called the C-serum. The top fraction phase contains the rubber particles. Biochemical smdies have established that latex in this fractionated form is unstable. These smdies also suggest that the bottom fraction is required for initiation of polymer synthesis. 

The filter centrifuge should not be confused with the sedimentation centrifuge widely used in biochemical laboratories. With a sedimentation centrifuge, fine precipitates are not filtered, but are pressed against the walls of the container and thus separated from the liquid. 

M. K. Brakke, Arch. Biochem. Biophys., 45 275 (1953). Zonal Separations by Density-Gradient Centrifugation. 

Ito, Y. Carmeci, P. Sutherland, I.A. Nonsynchronous flow-through coil planet centrifuge applied to cell separation with physiological solution. Anal. Biochem. 1979, 94, 249-252. 

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