Seeds proteins

Crawford, D. J. 1976. Variation in seed protein profiles of Chenopodium fremontii. Biochem. Syst. Ecol. 4 169-172. 

As noted above, the presence of Met(O) in proteins would go undetected after acid hydrolysis and subsequent amino acid analysis. Thus, since this method of hydrolysis is most commonly used, it is impossible to ascertain from the literature the abundance of Met(O) residues normally present in proteins. However, a number of studies have reported the presence of Met(O) residues in various proteins using one of the appropriate procedures described above. It has been found that Met(O) residues comprise 30% of the total Met in proteins isolated from bovine glomerular basement membranes and anterior lens . Other investigators have reported that the levels of Met(O) in proteins of the trabecular meshwork of human eyes increased with the age of the donor . The amount of Met(O) detected ranged from 15% (10 years old) to 55% (79 years old) of the total methionine content found in the tissue samples. Other studies have shown that in certain species of clams the proteins of the hinge ligament contain only Met(0) residues and no Met . In addition, it has also been reported that as much as 18% of the Met residues in pea seed proteins is in the form of Met(O) . Lastly, Met(O) residues have been found in... 

Lansbury PT Jr. Yeast prions inheritance by seeded protein polymerization Curr Biol 1997 7 R617-R619. 

The number of seeds per plant is large, and the seeds are small. The high seed protein content results in high yields of recombinant proteins. 

There are few reports of barley used for the production of recombinant proteins but some of those reports show high expression levels which, due to the low producer price of barley, could make this a viable production crop. In early reports, recombinant glucanase and xylanase were expressed at very low levels (0.004%) [79,80] but as stated above, a recombinant diagnostic antibody (SimpliRED, for the detection of HIV) has been expressed at levels exceeding 150 pg g-1 [15], and a recombinant cel-lulase enzyme was expressed at levels exceeding 1.5% total seed protein [16]. 

Human hemoglobin (< + P) CaMVenhanced 35S promoter/ CaMV 35S terminator Transit peptide of small subunit of RubisCO of Pisum sativum N. tabacum 0.05% of seed protein 37... 

Aprotinin Maize ubiquitin promoter/ pinll terminator Maize ubiquitin 5 intron, barley a-amylase LP Extracellular matrix of embryo (maize seeds) In T5 0.35% of extractable seed proteins 16... 

Hirudin Oleosin promoter/ nos terminator Fused to native oleosin B. napus 1% of seed protein 21... 

Legumes Soybean High seed protein content, seed coat expression, low producer price Lower expression levels... 

Melroy DL, Herman EM. TIP, an integral membrane protein of the soybean seed protein storage vacuole, undergoes developmentally regulated membrane insertion and removal. Planta 1991 184 113-122. 

Gottschalk, W. Muller, H. P. "Seed Proteins Biochemistry, Genetics, Nutritial Value". Martinus Nihhoff/Dr. W. Junk Publishers, The Netherlands, 1983. 

Daussant, J. Mosse J. Vaughan, J. "Seed Proteins". Academic Press, Inc., London, 1983. 

Defatted flours are especially attractive as protein sources, since 10-12% substitution of wheat flour with 50% protein flour will raise total protein content of typical wheat breads by approximately 50%, and 25% substitution will almost double the protein content of cookies. Preparation of protein-enriched breads has been reported in the literature using soy flours and protein concentrates (25), peanut flours and peanut protein concentrates C26, 27), glandless cottonseed flours, concentrates and isolates (28), sunflower seed flours and seed protein concentrates (27) and sesame flours and protein concentrates (26). 

Protein quality studies evaluating various edible portions of the winged bean plant and the results of recent International Field Trials are reviewed. Research efforts have been focused upon the mature seed (protein content 20.7-45.9% in 240 accessions), which is occasionally eaten in parts of Indonesia and Papua, New Guinea. Autoclaved seed meal and wet heat treated seed meal provided a corrected PER value of 1.76 and 1.72 respectively, in comparison to casein control of 2.50. 

Decorticated cucurbit seeds contain by weight about 35% protein. Traditionally, seed proteins are classified as globulins and albumins according to their solubility in certain aqueous solvents. Biochemically, oilseed globulins are generally considered storage proteins while albumins are believed to be metabolic (catalytic) proteins. 

From amino acid compositions, evaluations o7" the nutritional potentials of cucurbit meals and globulins can be calculated according to FA0/WH0 (54). The A E ratios, which are the amounts of each essential amino acid relative to the total amount of essential amino acids, are shown in Table II. These data indicate that, like most other oilseeds, cucurbit seeds are deficient in lysine and sulfur-containing amino acids. However, sulfur-containing amino acids are considerably high in CitrullI us colocynthis (egusi, ancestral watermelon) seed protein and exceed the suggested level in FA0/WH0 reference protein (55). 

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