Protein enrichment

Draw a flow diagram to show the production of protein-enriched feed from solid starch wastes. 

Adaptor protein enriched in the post-synaptic region of neurons. Important for assembly of receptor clusters and signaling complexes. 

The activity of G protein Py subunits is modulated by another protein termed phosducin [16]. Phosducin is a cytosolic protein enriched in retina and pineal gland but also expressed in brain and other tissues. Phosducin binds to G protein Py subunits with high affinity. The result is prevention of Py subunit reassociation with the a subunit. In this way, phosducin may sequester Py subunits, which initially may prolong the biological activity of the a subunit. However, eventually this sequestration may inhibit G protein activity by preventing the direct biological... 

Compositional differences in the pea seeds influence the quality of the end products. Pea flours have been used for protein enrichment of a number of cereal-based products however, undesirable sensory characteristics may limit their use, in spite of improved functional effects in food systems. The production of volatile compounds during cooking and baking of foods with pea supplementation affects their acceptability. Enzyme systems active in unheated pea flours may contribute to their functional properties, but adversely affect the sensory quality of the food. 

Much of the current interest in uses of derived oilseed proteins in compounded foods stems from projects in the mid-1960 s to alleviate massive world hunger. Perhaps the best known of these was the development of Incaparina at the Institute for Nutrition of Central America and Panama, in Guatamala by Bressani and coworkers (JJ. However, many other vegetable protein-enriched mass feeding foods also have been developed, and have been reviewed (2, 3). ... 

Defatted flours are especially attractive as protein sources, since 10-12% substitution of wheat flour with 50% protein flour will raise total protein content of typical wheat breads by approximately 50%, and 25% substitution will almost double the protein content of cookies. Preparation of protein-enriched breads has been reported in the literature using soy flours and protein concentrates (25), peanut flours and peanut protein concentrates C26, 27), glandless cottonseed flours, concentrates and isolates (28), sunflower seed flours and seed protein concentrates (27) and sesame flours and protein concentrates (26). 

Senti, F.R. In "Protein Enriched Cereal Foods for World Needs" Milner, M., Ed. American Association of Cereal Chemists St. Paul, MN, 1969 pp. 246-254. 

Piper, D.R., Duff, S.R., Eliason, H.C., Erazee, W.J., Erey, E.A., Euerstenau-Sharp, M., Jachec, C., Marks, B.D., Pollok, B.A., Shekhani, M.S., Thompson, D.V., Whitney, P., Vogel, K.W. and Hess, S.D. (2008) Development of the predictor hERG fluorescence polarization assay using a membrane protein enrichment approach. ASSAY and Drug Development Technologies, 6, 213-223. 

Figure 3 Analysis of selected fractions from DEAE column by 2-D PAGE. Fractions m, IV, and V from the anion-exchange separation of rat serum (Fig. 2) were analyzed by 2-D PAGE. Low-abundance proteins enriched by fractionation are noted with arrowheads.

Table 1 Search of a Protein Sequence Database With Ions Resulting from a MALDI-Tof Analysis of a Tryptic Digest of a Rat Serum Protein Enriched by Cation-Exchange Chromatography a...

M Fountoulakis, H Langen, S Evers, C Gray, B Takacs. Two-dimensional map of Haemophilus influenzae following protein enrichment by heparin chromatography. Electrophoresis 18 1193-1202, 1997. 

Several approaches relying on affinity chromatography allows for selective protein enrichment including posttranslational-modified proteins and membrane proteins and also can be utilized for depleting unwanted high-abundance proteins. These approaches can be readily inserted as an... 

Do you think that the people of Ridimbo would be interested in a GE protein-enriched millet or vitamin A-enriched rice I ask. 

R. Shiloach (personal communication, 1999) believes that ion-exchange adsorbents are a powerful tool in the purification of proteins. They can be used in the initial steps of the protein recovery process by capturing the proteins, enrichment, or polishing. It is likely that new and improved adsorbents will continue to be introduced. 

The ability to handle low sample volumes has become essential in order to gain high yield in sensitivity amplification by different types of protein enrichment technologies. This thereby provides several benefits ... 

Most proteomics analyses involve 2-D electrophoresis and MALDl-TOF MS steps, as this approach allows for a reliable quantification of changes in protein levels. In one study, the LC-MS/MS approach was used for the detection of proteins enriched in amyloid plaques in the AD brain (liao et al., 2004). Employing the former proteomic approach, levels of about 100 proteins were foimd to be changed in the AD brain and cerebrospinal fluid (CSF) in comparison with the control brain and CSF, respectively (Table 15.1). These proteins have various functions, mainly involved in neurotransmission, guidance, signal transduction, metabolism, detoxification, and conformational changes. The CSF proteins are plasma proteins. 

Coronin, achn binding protein Enriched in amyloid plaques Liao et al. 2004... 

Using LC-MS/MS, Liao et al. (2004) identified 26 brain proteins enriched in amyloid plaques excised with laser capture microdissection from AD brains. 

---