Seed Lines

In January 2008, Shim and Wanasundara announced their development of a sandwich ELISA that targets the Sin a 1 allergen from S. alba [11]. The assay was developed to quantify Sin a 1 levels in various yellow mustard seed lines, but was reported to be less sensitive than the previously described B.juncea assay developed by Koppleman et al. [6]. 

Those T2 lines showing the greatest increases in seed oil content and proportions of VLCFAs were propagated to give T3 progeny lines. Several SLCl-1 T3 seed lines contained significantly increased lipid content (pg total fatty acids/100 seeds) compared to pBI121 controls (Fig. 1). 

B. napus cv. Hero SLCl-1 Transformant Seed Lipid Analyses B. napus cv. Hero SLCl-1 T2 seed lines exhibited 10-25% increases in oil content and 4-10% increases in erucic acid content. 

The complete system contains 0.3 mg ribosomes from dry seeds (lines 1-5) or 48-h imbibed seeds (line 6), 0.8 mg supernatant (cytoplasm) protein, 125 pg mRNA, 0.5 pCi " C-leucine, 10-15 pmol/ml Mg. Incubation at 37° for 30 min. Cycloheximide (15 pg), an inhibitor of protein synthesis on 80S ribosomes, is added to one tube. The effect of omitting ribosomes was not tested. Based on Jachymczyk and Cherry, 1968 [62]... 

Sesamin has been associated with the reduction of serum and liver lipids (Hirose etal., 1991,1996 Ide etal., 2001 Yasumoto etal., 2001). Hirose etal. (1991) reported that sesamin decreased lymphatic absorption of cholesterol. Sesamin was found to be a promoter of hepatic fatty acid oxidation in rats (Ashakumary et al., 1999). Addition of a 1 1 mixture of sesamin and episesamin to the diet of rats (0.1-0.5% of total diet) promoted mitochondrial and peroxisomal palmitoyl coenzyme A (CoA) oxidation rates in a dose-dependent manner. Rats fed diets containing 0.5% sesamin had 2 fold higher mitochondrial activity and 10 fold higher peroxisomal activity than rats fed non-sesamin diets. Yasumoto et al. (2001) reported similar findings when a 20% sesame seed diet was fed to rats. These authors compared two experimental sesame seed lines (0730 and 0732) with the commercial cultivar Masekin. Both experimental seed lines enhanced mitochondrial and peroxisomal fatty acid oxidation rates, and were more effective than the commercial cultivar at reducing plasma triacylglycerol levels (Yasumoto et al., 2001). A study of the effects of dietary sesamin on the hepatic metabolism of arachidonic and eicosapentaenoic acids in rats showed similar increases in hepatic mitochondrial and peroxisomal fatty acid oxidation (Sawada et al., 2001). 

The separation of the solid phase does not occur readily with some liquid mixtures and supercooling is observed. Instead of an arrest in the cooling curve at /, the cooling continues along a continuation of c/ and then rises suddenly to meet the line f g which it subsequently follows (Fig. 1,13, 1, iii). The correct freezing point may be obtained by extrapolation of the two parts of the curve (as shown by the dotted line). To avoid supercooling, a few small crystals of the substance which should separate may be added (the process is called seeding ) these act as nuclei for crystallisation. 

Although 16 different crystalline modifications have been identified (24,25), the a-pentahydrate is the stable form below 48°C. Solutions of sodium thiosulfate in the absence of seed crystals can be easily supercooled below their normal crystallisation temperatures. The dotted line extension of the dihydrate phase in Figure 1 is an indication that, if supercooling takes place below this line, solutions normally giving the pentahydrate may form the dihydrate [36989-90-9] s1ste2id. 

Continuous emulsion copolymerization processes for vinyl acetate and vinyl acetate—ethylene copolymer have been reported (59—64). CycHc variations in the number of particles, conversion, and particle-size distribution have been studied. Control of these variations based on on-line measurements and the use of preformed latex seed particles has been discussed (61,62). 

The Berlin City electrical engineer M. Kallmann reported in 1899 on a system for controlling stray currents of electric railways [64]. As early as 1894, the Board of Trade in London issued a safety regulation for the British electric railways which specified a potential differential of not more than 1.5 V where the pipeline was positive to the rails, but 4.5 V with the rails positive. Extensive research was undertaken on reducing the risk of stray current in the soil by metallic connections from pipes to rails. However, as one writer noted, a procedure on these lines should definitely be discouraged as it carries the seed of its own destruction [64]. 

The shape of the equilibrium line, or solubility curve, is important in determining the mode of crystallization to be employed in order to crystallize a particular substance. If the curve is steep, i.e. the substance exhibits a strong temperature dependence of solubility (e.g. many salts and organic substances), then a cooling crystallization might be suitable. But if the metastable zone is wide (e.g. sucrose solutions), addition of seed crystal might be necessary. This can be desirable, particularly if a uniformly sized product is required. If on the other hand, the equilibrium line is relatively flat (e.g. for aqueous common salt... 

Figure 5.15, Pressure in horizontal 25 rnni conveying line for Iransport of cress seed...

The seeding procedure is described in Fig. 20 by the line abed. Its inspection shows that the concentration of the monomer left at the time when all the initiator is consumed remains the same whether the monomer is added at once or in two portions. Moreover, the total concentration of the added monomer must exceed a critical value to allow for quantitative consumption of the initiator. Thus, the seeding technique does not eliminate the broadening of molecular weight distribution caused by slow initiation of a virtually irreversible polymerization. This conclusion is confirmed experimentally 133). 

The tamarind seed XG exerts several biological activities, such as marked inhibitory effect on the binding of BK virus to cells [299] and immunomodulatory effects [300]. Recently, it was reported [301] that XG affects the proliferation of cytokines in various skin-cell lines (Fig. 14) such as HaCaT cells (im-... 

Fig. 14 Effect of the molecular weight of tamarind seed xyloglucan depolymerized by ( ) 7-irradiation, ( ) ultrasonication, and ( ) endo-glucanase treatment on the production of various cytokines (Tumor necrosis factor a, TNE-a Interleukin 8, IL-8 Interleukin 10, IL-10 and Interleukin 12, IL-12) in HaCaT cells (Immortalized keratinocytes line) [301]...

Tissue Culture Assay. Kogure et al. (48) report a novel tissue culture assay for detecting several types of sodium channel blockers. The mouse neuroblastoma cell line ATCC CCL 131 is grown in RPMI 1640 supplemented with 13.5% fetal bovine serum and 100 pg/ml gentamycin, in an atmosphere of 5% C0 95% air at 37 C. Ninety-six well plates are seeded with 1 x 10 cells in 200 pi of medium containing 1 mM ouabain and 0.075 mM veratridine. Veratridine and ouabain cause neuroblastoma cells to round-up and die. In the presence of sodium channel blockers (e.g., TTXs or STXs), the lethal action of veratridine is obviated and cells retain normal morphology and viability. An important feature of this assay is that a positive test for sodium channel blockers results in normal cell viability. Since bacterial extracts can contain cytotoxic components, this assay offers an advantage over tests that use cell death as an endpoint. The minimum detectable level of TTX is approximately 3 nM, or approximately 1/1000 mouse unit. 

Many in vitro studies have been undertaken to explore the relative effectiveness of isothiocyanates, and associated compounds, as inhibitors of cell growth and inducers of apoptosis in cell lines, and some of these are summarised in Table 4.2. Clonal survival studies are often used to determine whether isothiocyanates inhibit initial cell anchorage and subsequent growth of sparsely seeded cells. However, differences in cell number following challenge with the isothiocyanate could be due either to decreased cell division or increased cell loss, and the authors of some in vitro studies have failed to recognise this. Other studies have, however, considered the interacting... 

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