Of sucrose solutions

Equations have been developed that determine the relationship of the refractive index of sucrose solutions between 0—85% concentration, 18—40°C, and 546—589 nm. 

Place 10 mL of sucrose solution and 10 mL of starch into test tubes 5 and 6. Add 2 drops of concentrated sulfuric acid to each solution and stir to mix thoroughly. Place the test tubes into the boiling-water bath and heat for 3 minutes. 

The glass transition of solutes that remain amorphous during and after the freezing process can often be seen in the DSC thermogram as a shift in the baseline toward higher heat capacity. This is illustrated in the DSC thermogram of sucrose solution in Fig. 6, in which the glass transition is observed at — 34°C. 

Linepithema humile W-PvG Previously identified trail pheromone enhances consumption of sucrose solution (9Z)-Hexadec-9-enal 66 [179]... 

Luck, W.A.P. 1981. Structures of water in aqueous systems. In Water Activity Influences on Food Quality (L.B. Rockland and G.F. Stewart, eds), pp. 407 134. Academic Press, New York. Ludescher, R.D., Shah, N.K., McCaul, C.P., and Simon, K.V. 2001. Beyond Tg Optical luminescence measurements of molecular mobility in amorphous solid foods. Food Hydro colloids 15, 331-339. Ludwig, R. 2001. Water From cluster to the bulk. Angewandte Chem. Int. Ed. 40, 1808-1827. Maclnnes, W.M. 1993. Dynamic mechanical thermal analysis of sucrose solutions. In The Glassy State in Foods (J.M.V. Blanshard and PJ. Lillford, eds), pp. 223-248. Nottingham Univ. Press, Loughborough, Leicestershire. 

Spin the samples with 200 000 x g at 4 °C for 15 -17 h. After the run, displace the gradient using a more dense solution, e.g., 40% sucrose in Soln. A, colored by a droplet Amido Black 10 B solution. The principle of a displacement apparatus is shown in Fig. 5.4. The RNA content of the fractions is measured either by reading the UV absorption at 260 nm or, if labeled material was used, by counting the radioactivity. To monitor the sucrose gradient, estimate the refractive index of the obtained fractions (concentration, density and refractive index of sucrose solutions are given in Table 8.17). 

Table 8.17. Concentration, density q, and refractive index n (20 °C, sodium light source) of sucrose solutions...

Solutions of synthetic polyglucoses have also been used for density-gradient centrifugation of viruses. 16 These substances are especially suited to this purpose, since they can be dissolved in water or buffer to yield solutions of high concentration and low intrinsic viscosity and a density similar to that of sucrose solutions. Because of their low rate of diffusion, polyglucoses form gradients that are more stable. A polyglucose nitrate possessed explosive properties, similar to those of starch nitrates and cellulose nitrates.200... 

Rotatory dispersion of sucrose solutions [SUGAR - SUGARANALYSIS] (Vol 23)... 

Table H1.3.2 Viscosities of Sucrose Solutions Measured by a Capillary Viscometer"...

Viscosity will vary depending on the sample. Typical results for viscosity of sucrose solutions are shown in Table HI. 3.2. 

Kett, V.L., Craig, D.Q.M. The effect of annealing on the glass trasition region of sucrose solutions. In Proc. NATAS Annu. Conf. Thermal Anal. Appl., 28th, pp. 653-658, 2000... 

Measure the volumes of sucrose solution and water consumed. Calculate the preference for the sucrose solution as a percentage of total liquid consumed and total sucrose intake in mg/g body weight. In addition, commercially available automated lick-counters (lickometers) may be used (e.g., by Lafayette Instrument Co, Lafayette IN, United States or Columbus Instruments, Columbus OH, United States). Assess the number of licks at each bottle for the duration of the test (i.e., 24-72 h) per 100 mg of body weight, and the preference for sucrose as a percentage of total licks (23, 27, 28) (see Notes 4-6). 

A standard assay for both forms of enzyme consisted of mixing 108 mL of sucrose solution (120 g/L in 0.010 M acetate buffer, pH 5.5) with 12 mL of aqueous invertase solution (diluted 1 5000 [v/v]) or 12 mL of an aqueous suspension of Dowex/invertase (100 mg of powder/mL). Hydrolysis was carried out for 6 min at 37°C under agitation (lOOrpm), as previously described by Vitolo et al. (1). One soluble or immobilized invertase unit (U) was defined as the amount of total reducing sugar (TRS) (mg) formed per minute under the conditions of the test. 

In 1861, Louis Pasteur1 reported a polysaccharide that was produced from sucrose. In 1874, Scheubler2 determined its empirical formula and named it dextran. The formation of polysaccharide (dextran) was observed as the result of bacterial transformation of sucrose solutions into viscous solutions, gels, and/or flocculent precipitates.3 The synthesis of dextran from sucrose by a cell-free... 

Small quantities of compounds in natural extracts are often a problem when these need to be evaluated in bioassays. Sometimes there is just not enough of the compound isolated to carry out the usual bioassay.20 Microassays have been developed10 1 to overcome this problem. Typically, a microassay is carried out on a thin-layer chromatography plate with a cellulose layer. A small droplet (1.5 pi) of the tested compound in a solvent (1-102 nmol cm-2) is then added on the plate. After the solvent has evaporated a small amount (5 pi) of sucrose solution lmoll-1 is added to the place where the compound was added. In the control the same procedure was followed on a different plate, but with the solvent alone, with no compound added. These two plates are then placed in a petri dish with the test insect species. In the past, when paper chromatography was widely used, a crude plant extract was placed on the origin of the paper and then eluted into bands. The paper was freed of solvent, sprayed with sugar solution, and used directly in a bioassay to see which parts of the paper were not eaten, and therefore of interest for further examination. 

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