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Scaffold technique

The scaffolding technique still requires intense validation studies to develop and optimize the different synthesis conditions required for the different backbones and ligand systems. Different electronic and steric properties may lead to synthetic conditions and in some cases even to alternative synthetic routes to arrive at a given ligand structure. [Pg.387]

Fig. 3.7. Scaffold technique. This technique is used for high flow vessels when there is concern about migration of a softer coil. Initially in this diagram a high radial force coil, with a diameter 2 mm larger than the artery or vein being occluded, is placed. Several high radial force coils may be placed and the occlusion is completed using Nester coils to tightly pack within the scaffold or endoskeleton ... Fig. 3.7. Scaffold technique. This technique is used for high flow vessels when there is concern about migration of a softer coil. Initially in this diagram a high radial force coil, with a diameter 2 mm larger than the artery or vein being occluded, is placed. Several high radial force coils may be placed and the occlusion is completed using Nester coils to tightly pack within the scaffold or endoskeleton ...
PAVM with normal flow PAVM with high flow Scaffold technique Anchor technique Anchor technique Vein of Galen technique... [Pg.293]

D.ii.d. [3+2] Cyclizations. The capability of [3 + 2] cycloaddition based on trimethyl-enemethane has served as a useful scaffolding technique in natural products and... [Pg.460]

K. Omori, Y. Tada, T. Suzuki, Y. Nomoto, T. Matsuzuka, K. Kobayashi, T. Nakamura, S. Kanemaru, M. Yamashita, R. Asato, Clinical application of in situ tissue engineering using a scaffolding technique for reconstruction of the larynx and trachea, Ann. Otol. Rhinol. Laryngol. 117 (9) (September 2008) 673—678. [Pg.564]

Human osteoblast-like MG63 cells were cultured on the macroporous chitosan scaffolds reinforced with hydroxyapatite or calcium phosphate invert glass were fabricated using a thermally induced phase separation technique. [Pg.171]

The cylindrical porous scaffolds based on TMC-DLLA copolymers were prepared by salt-leaching method. Two-ply porous nerve guides were prepared by a combination of dip-coating (inner layer) and fiber-winding (outer layer) techniques. [Pg.230]

There are two approaches to synthesizing hydrophilic carotenoids (1) appending a hydrophilic group to the carotenoid scaffold (Foss et al. 2006a) or (2) joining a carotenoid to a hydrophilic compound, Scheme 3.3 (Foss et al. 2003). Whereas the Scheme 3.3 intuitively explains the difference, these techniques cannot be clearly separated in praxis the distinction may appear more emotional than conceptual. Both methods are habitually hampered by low yields, find their limits in the availability of functionalized carotenoids, and cause problems in the work-up procedure due to the amphiphilic character of the products. [Pg.34]

Fabrication approaches have been previously used in two-dimensional (2D) micropattemed model systems and have led to insights on the effect of cell-cell and cell-polymer scaffold interactions on hepatocyte and endothelial cell fate. Extending these studies, the application of 3D fabrication techniques may also prove useful for studying structure-function relationships in model tissues. [Pg.144]

Fig. 5. (a) Schematic of the scaffold design showing the inner and outer scaffolds, (b and c) Inner scaffolds seeded with NSCs. (Scale bars 200 pm and 50 tm, respectively.) The outer section of the scaffold was created by means of a solid-liquid phase separation technique that produced long, axially oriented pores for axonal guidance as well as radial pores to allow fluid transport and inhibit the ingrowth of scar tissue (d scale bar, 100 pm), (e) Schematic of surgical insertion of the implant into the spinal cord. [Reproduced with permission from Teng et al. (2002).]... [Pg.62]

A method was recently developed to deliver plasmid DNA locally to cells involved in wound repair (Bonadio et al., 1999 Fang et al., 1996 Shea et al., 1999). The technique involves the introduction of a porous, biodegradable polymer matrix into the wound site (Bonadio et al., 1998). The scaffold (a gene activated matrix, or GAM), in its simplest form comprises plasmid DNA and the polymer matrix (Bonadio, 2000). Naked... [Pg.157]

Carbohydrates not only act as ligands, but they can also provide scaffolds for molecular recognition processes. It is well known that cyclodextrins (CDs) are able to form an inclusion complex with specific guest molecules. In the last years, NMR experiments combined with other techniques have been used to highlight different recognition events. [Pg.348]

Even with the knowledge of the reactive moieties that are suspected to trigger M BI, there are numerous potential pathways for the chemistry to lead to protein inactivation [174,196]. Differentiating these mechanisms can facilitate the generation of alternate and safer chemical scaffolds. The UV-Vis spectrophotometer has been a key instrument in activity and functional characterization for CYPs for the past 40 years, as indicated by the derivation of its name, pigment 45 0 being the signature UV band present when reduced in the presence of CO [5,197]. This technique has been... [Pg.227]

Figure 12.5 indicates the results of this technique when applied to three MBIsand three non-MBI compounds. The three MBIs are clearly recognized and the electrophilic intermediate leading to MBI clearly reported. The three non-MBI compounds are also well predicted (as non-M Bis) despite the presence on the molecular scaffold of well-known potential electrophilic intermediates. Similar to the prediction of the site of metabolism, the example demonstrates that the recognition component fc) is more important than the reactivity component to determine if a compound may lead to MBI. [Pg.286]


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