Reverse-phase high-performance liquid assay

Van Kuilenburg ABP, Zoetekouw L (2005) Determination of thymidine phosphorylase activity by a non-radiochemical assay using reversed-phase high-performance liquid chromatography. J Chromatogr Biomed Sci Appl 820 271-275... [Pg.738]

A reversed-phase high performance liquid chromatographic method for the determination of salvinorin A, a psychotropic diterpene isolated from the Mexican sage Salvia divinorum, has been developed. Extracts from several plant collections were examined on a C-18 column with UV detection and isocratic elution with acetonitrile water (45 55). This assay allowed quantification of salvinorin A in extracts of leaves and stems of A , divinorum and has also been applied to the screening of related species for the production of salvinorin A. Levels of salvinorin A in leaves range from 0.89 to 3.70 mg/g dry weight. 1999 John Wiley Sons, Ltd. [Pg.252]

W. N. Barnes, A. Ray, and L. J. Bates, Reversed-phase high-performance liquid chromatographic method for the assay of oxy-tetracycline, J. Chromatogr., 347 173 (1985). [Pg.251]

J. Farquhar, G. Finlay, P. A. Ford, and M. Martin-Smith, A reversed-phase high-performance liquid chromatographic assay for the determination of N-acetylcysteine in aqueous formulations, J. Pharm. Biomed. Anal., 3 279 (1985). [Pg.437]

Synthetic peptides often lack the conformational stability required for a successful drug therefore determination of peptide stability in serum constitutes a powerful and important screening assay for the elimination of unstable peptides in the pipeline of drug development (see Note 1). Peptide stability in serum can rather easily be determined by reverse phase-high-performance liquid chromatography (RP-HPLC) and mass spectroscopy (MS) from both in vitro and in vivo studies. [Pg.178]

The inosine and hypoxanthine were separated by reversed phase high performance liquid chromatography and the amount of hypoxanthine produced was related to the phosphate concentration. Quantitation of the hypoxanthine peak was found to be linear with orthophosphate up to about 30mg L A detection limit of 0.75mg L 1 could be obtained after dialysis of the commercial enzyme. Interference studies showed that the enzymatic assay unlike the colorimetric molybdate blue technique was essentially unaffected by complex matrices such as polyphosphates and phosphoesters. [Pg.154]

O Keefe, D. O., and Paiva, A. M. (1995). Assay for recombinant hepatitis B surface antigen using reversed-phase high-performance liquid chromatography. Anal. Biochem. 230, 48-54. [Pg.66]

Procyanidins with different degrees of polymerization such as dimers, trimers, and polymers from extracts of different natural sources were found to be nonmutagenic in the Salmonella mutagenesis assay. Moreover, the mutagenic impurity in procyanidin B-4 was isolated by reversed-phase high-performance liquid chromatography (HPLC). Procyanidin B-4 was identified as rutin (flavonol O-glycoside, 3) (Fig. 22) [55]. [Pg.36]

Bohler U, Bind E, Vogel G, Hitzler W, Flentje D, Schmidt-Gayk H. Two-site immunochenulumino-metric assay of intact human parathyrin in serum with use of a tracer peptide purified by reversed-phase high-performance liquid chromatography. [Pg.1945]

A. F. Fell and S. M. Plag, Stability-indicating assay for azathioprine and 6-mercaptopurine by reversed-phase high-performance liquid chromatography, J. Chromatogr. 186, 691-704(1979). [Pg.232]

Yuan, Z. Russlie, H.Q. Canafax, D.M. Sensitive assay for measuring amoxicillin in human plasma and middle ear fluid using solid-phase extraction and reversed-phase high-performance liquid chromatography. J.Chromatogr.B, 1995, 674, 93-99... [Pg.94]

Nitsche, V. Mascher, H. New rapid assay of cimetidine in human plasma by reversed-phase high-performance liquid chromatography. J.Chromatogr., 1983, 273, 449-452... [Pg.336]

Kabra, P.M. Wall, J.H. Dimson, P. Automated solid-phase extraction and liquid chromatography for assay of cyclosporine in whole blood. Clin.Chem., 1987, 33, 2272-2274 Sangalli, L. Bonati, M. Reversed-phase high-performance liquid chromatography determination of cyclosporin in human blood. Ther.Drug Monit., 1987, 9, 353-357 Shibata, N. Minouchi, T. Hayashi, Y Ono, T. Shimakawa, H. Quantitative determination of cyclosporin A in whole blood and plasma by high performance liquid chromatography. Res.Commun. Chem.Pathol.Pharmacol., 1987, 57, 261-271... [Pg.456]

Gutenberger, S.K. Olson, D.P. Kagel, R.A. Comparison of reversed phase high-performance liquid chromatography and competitive protein binding assay in the quantification of cortisol in bovine plasma. J.Liq.Chromatogr., 1985, 8, 107-124 [plasma cow dexamethasone (IS)]... [Pg.740]

Rustum, A.M. Assay of ibuprofen in human plasma by rapid and sensitive reversed-phase high-performance liquid chromatography application to a single dose pharmacokinetic study. J. Chromatogr.Sci., 1991, 29, 16-20... [Pg.752]

Schuster, D. Modi, M.W. Lalka, D. Gengo, F.M. Reversed-phase high-performance liquid chromatographic assay to quantitate diastereomeric derivatives of metoprolol enantiomers in plasma. J.Chromatogr., 1988, 433, 318-325... [Pg.909]

Chen, T.-L. Kennedy, M.J. Dunlap, V.M. Colvin, O.M. Determination of plasma novobiocin levels by a reversed-phase high-performance liquid chromatographic assay. J.Chromatogr.B, 1994, 652, 109-113... [Pg.1158]

Lau-Cam, C.A. Rahman, M. Roos, R.W. Rapid reversed phase high performance liquid chromatographic assay method for ranitidine hydrochloride in dosage forms. J.Liq.Chromatogr., 1994,17,1089-1104... [Pg.1216]

Chiang, J.Y. (1991). Reversed-phase high-performance liquid chromatography assay of cholesterol 7 alpha-hydroxylase. Meth. Enzymol. 206, 483 91. [Pg.656]

Lehmberg E, Traina J A, Chakel J A, et al. (1999). Reversed-phase high performance liquid chromatographic assay for the adenovirus type 5 proteome. J. Chromatog. B. 732 411-423. [Pg.970]

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