Assays radiochemical

This enzyme is a biotin protein. It also catalyses transcarboxylation reactions. The reaction shown here is an important step in fatty acid synthesis utilising malonyl CoA as an intermediate. It may be assayed radiochemically using C labelled KHCO3 in a reaction which is stopped by the addition of 6 N HCl [180]. 

This is one of the enzymes of the urea cycle, which may be assayed radiochemically using C labelled guanido-arginosuccinate [241]. 

This enzyme catalyses the reaction which is the Hrst in the sequence which leads to the synthesis of pyrimidines. It may be assayed radiochemically (279) by using labelled carbamoylphosphate. The reaction mixture is acidified and heated to eliminate residual substrate as 002 and the acid stable product counted by liquid scintillation techniques. 

This enzyme catalyses one of the reactions involved in the metabolism of catecholamines. It can be assayed radiochemically using C labelled S-adenosylmethionine. The reaction mixture is reacted with dihydroxybenzoic acid and the unused substrate as a benzoate is extracted with organic solvent at acid pH. 

This enzyme catalyses the reaction whereby acetylcholine is synthesised in tissue. It will also react with proprionyl co-enzyme A, but more slowly. It may be assayed radiochemically using C labelled acetyl CoA. Residual acetyl CoA is decomposed by hydroxylamine, and the labelled acetylcholine is precipitated with sodium tetraphenyl-... 

This enzyme catalyses an important step in the synthesis of sulphated mucopolysaccharides. It can be assayed radiochemically by measuring incoipoiation of 3 -phospho-adenylyl sulphate labelled with S into chondroitin-4-sulphate utilising paper chromatography to separate substrate and product. [333]. 

This is a transaminase which utilises 2-oxoglutarate and which can be assayed radiochemically by modification of the method given for ALANINE AMINOTRANSFERASE. 

A DNA chain acts as a primer, and the enzyme forms a complementary chain. It can be assayed radiochemically by using nucleotide donors labelled with C, H, or and determining incorporation into acid insoluble precipitates [356-357]. 

This enzyme is a pyridoxal phosphate protein which can also catalyse reactions with hydroxyphenylalanine and 3-hydroxyphenylserine. It catalyses the formation of dopamine which is the precursor of noradrenaline. It may be assayed radiochemically utilising the evolution of C labelled CO2. 

This enzyme catalyses the conversion of dopamine to noradrenaline. It may be assayed radiochemically using H labelled dopamine. 

This enzyme is a pyridoxal phosphate protein, which catalyses the formation of 7-aminobutyric acid, a possible chemical transmitter in the CNS. It can be used in an indicator reaction for the radiochemical assay of transaminases. It is assayed radiochemically using Clabelled glutamate and COj evolution [391]. 

This enzyme is activated by D-glucose-6-phosphate and other hcxose phosphates. It catalyses the last step in glycogen synthesis, and may be assayed radiochemically by measuring the incorporation of C labelled UDP-glucose into glycogen, which is precipitated at the end of the reaction [422]. 

This is a pyiidoxal phosphate protein which catalyses histamine synthesis. It can be assayed radiochemically utilising evolution of C02 [433]. 

This is the enzyme which catalyses the formation of mevalonate, an important intermediate in the formation of cholesterol from acetyl CoA. It can be assayed radiochemically using C labelled substrate and ether extraction of the product. 

This is the enzyme responsible for activating methionine to the 5-adenosyl form in which it acts as a methyl donor. It may be assayed radiochemically using C labelled methionine and product separation using ion exchange. 

This enzyme is a cuproprotein which acts on a variety of primary, secondary, and tertiary amines. It is involved in the metabolism of serotonin, catecholamines, and many other drugs. It may be assayed radiochemically using 0 labelled serotonin, tyramine or dopamine, and solvent extraction of the reaction mixture [508. ... 

This enzyme contains tightly bound cyanocobalamin which imparts a salmon pink colour. It catalyses one of the reactions which generate methionine from homocystein, and is thus involved indirectly in transmethylation. It may be assayed radiochemically. 

This is an enzyme of the Krebs—Henseleit urea cycle which occurs almost exclusively in liver. It is currently considered to be a good specific indicator of hepatic cell damage. In acute carbon tetrachloride poisoning in swine serum levels are elevated 400-fold. In dogs, the elevation is greater (2000-fold). It may be assayed radiochemically [516] or... 

This enzyme requires DNA as a primer. It catalyses the reaction responsible for chain lengthening in RNA synthesis. It may be assayed radiochemically using C, H or P labelled nucleotide donors and acid precipitation of the product. 

MAD activity was assayed radiochemically accordingly to Leech et al,. was measured enzymatically by the assay provided by Boehringer. 

K. G. Oldham, Radiochemical Methods of Enzyme Assay, Radiochemical Centre, Amersham, 1968... 

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