Resveratrol determination

An amperometric biosensor for trans-resveratrol determination in aqueous solutions by means of carbon paste electrodes modified with peroxidase basic isoenzymes (PBIs) from Brassica napus was developed [182]. Catalytic properties of PBIs from Brassica napus toward trows-resveratrol oxidation were demonstrated by conventional UV-vis spectroscopic measurements. The enzymatic reaction rate was studied and kinetics parameters were detected. An amperometric biosensor based on Brassica... 

Xiang, H.Y. Li, W.G. (2009). Electrochemical sensor for trans-resveratrol determination based on indium tin oxide electrode modified with molecularly imprinted self-assembled films. Electroanalysis, 21,1207-1210. 

Lopez-Nicolas JM and Garcia-CarmonaF. 2008. Aggregation state and pKa values of (E)-resveratrol as determined by fluorescence spectroscopy and UV-visible absorption. J Agric Food Chem 56(17) 7600-7605. 

Lopez-Nicolas JM, Nunez-Delicado E, Perez-Lopez AJ, Carbonell A and Cuadra-Crespo P. 2006. Determination of stoichiometric coefficients and apparent formation constants for (3-cyclodextrin complexes of trans-resveratrol using reversed-phase liquid chromatography. J Chromatogr A 1135 158—165. 

To resolve the issue of cyclization specificity, the x-ray crystal structure of the stilbene synthase from pine was determined to atomic resolution. This information allowed the mutagenic conversion of alfalfa CHS to a functional STS, and crystal structures of this engineered STS were solved, in the apo form and with resveratrol bound in the active site (Austin and Noel, unpublished). These experiments support a mechanistic proposal, which prompted further mutagenic and modeling experiments. This work has allowed the elucidation of the structural and mechanistic basis for cyclization specificity (aldol versus Claisen condensation) in the CHS family of type III PKSs. 

Another study employed a similar RP-HPLC method for the determination of trails- and d.v-rcsvcratrol, catechin, epicatechin, quercetin and rutin in wines and musts. Wine samples were filtered and diluted when necessary and used for analysis without any other pretreatment. Separation was performed in an ODS column (150 X 4 mm i.d. paricle size 5 71m) at ambient temperature. The gradient began with ACN-5 per cent aqueous acetic acid (9 91, v/v) for 0-10 min to 25 75 in 1 min hold for 11 min to 70 30 in 1 min, hold for 5 min. The flow rate was 1 ml/min. Analytes were detected by DAD. Fluorescence detection used 280/315 nm (excitation/emission) for catechin and epicatechin 314/370 nm for fims-resveratrol and 260/370 nm for d.v-rcsvcratrol. Chromatograms of a red wine sample obtained at different... 

Yu, C., Shin, Y.G., Kosmeder, J.W., Pezzuto, J.M., and van Breemen, R.B., Liquid chromatog-raphy/tandem mass spectrometric determination of inhibition of human cytochrome P450 isozymes by resveratrol and resveratrol-3-sulfate. Rapid Commun. Mass Spectrom., 17, 307, 2003. 

Emilia, J. M., Lamuela-Raventos, R. M., and de la Torre-Boronat, C. M., 1999, Determination of tram-resveratrol in plasma by HPLC, Anal. Chem. 71 747-750. 

Yitrac X, Bornet A, Yanderlinde R, Vails JM, Richard T, Delaunay J-C, Merillon J-M, Teissedre P-L. 2005. Determination of stilbenes (8-viniferin, trans-astringin, trans-piceid, cis- and trans-resveratrol, e-viniferin) in Brazilian wines. J Agric Food Chem 53 5664-5669. 

In the first kinetic study, Juan et al. [2002] focused on the determination of the time course of /ruH.v-resvcratrol level in plasma after the 2-mg/kg orally administrated dose to rats. Resveratrol had already reached the bloodstream at 5 min, presented its maximum level at around 10 min, and was still detected after 60 min [Juan et al., 2002]. 

Chen X, He H, Wang G, Yang B, Ren W, Ma L, Yu Q. 2007. Stereospecific determination of cis- and traws-resveratrol in rat plasma by HPLC Application to pharmacokinetic studies. Biomed Chromatogr 21 257-265. 

Huang H, Zhang J, Chen G, Lu Z, Wang X, Sha N, Shao B, Li P, Guo DA. 2008. High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract. Biomed Chromatogr 22 421-427. 

Tokusoglu O, Unal MK, Yemis F. 2005. Determination of the phytoalexin resveratrol (3,5,4 -trihydroxystilbene) in peanuts and pistachios by high-performance liquid chromatographic diode array (HPLC-DAD) and gas chromatography-mass spectrometry (GC-MS). J Agric Food Chem 53 5003-5009. 

Zhou M, Chen X, Zhong D. 2007. Simultaneous determination of fra w-resveratrol-3-0-glucoside and its two metabolites in rat plasma using liquid chromatography with ultraviolet detection. J Chromatogr B Analyt Technol Biomed Life Sci 854 219-223. 

Stivala LA, Savio M, Carafoli F, Perucca P, Bianchi L, Maga G, Forti L, Pagnoni UM, Albini A, Prosperi E, Vannini V. 2001. Specific structural determinants are responsible for the antioxidant activity and the cell cycle effects of resveratrol. J Biol Chem 276 22586-22594. 

Gu, X. Kester, A. Zeece, M. 1999. Capillary electrophoretic determination of resveratrol in wines. J. Agric. Food Chem. 47 3223-3227. 

Since Siemann and Creasy (1992) described the presence of /rani-resveratrol in wines, many different methods have been developed to determine this compound and its derivatives (Romero-Perez et al. 1996). Various methods use RP18-HPLC and gradient elution with UV detection (Lamuela-Raventos et al. 1995 Naugler et al. 2007 Ribeiro de Lima et al. 1999 Vitrac et al. 2005). 

Ribeiro de Lima, M. T, Waffo-Teguo, R, Teissedre, R. L., Rujolas, A., Vercauteren, J., Cabanis, J. C., Meiillon, J. M. (1999). Determination of stilbenes (irans-astiingin, cis- and trani-piceid, and cis- and irans-resveratrol) in Rortuguese wines. J. Agric. Food Chem., 47, 2666-2670. 

To investigate the probable health benefits of flavonoids and stilbenes in red wine, a RP HPLC method with enhanced separation efficiency, selectivity, sensitivity, and speed has been established for the determination of the flavonols quercetin, myricetin, and kaempferol and the stilbenes cis- and trany-resveratrol in a single The... 

Dihydroxy-3-methylbutyl)resveratrol (69) from an ethyl acetate extract ofthe bark has been determined to have absolute... 

Another six novel flavonostilbenes, alopecurones A-F (782-787), were isolated from the roots of Sophora alopecuroides. Their structures are composed of a flavanone condensed with a resveratrol (1). Absolute configurations of alopecurones A-F (782-787) were determined by means of CD compared with model compounds [342]. 

The application of high performance liquid chromatography (HPLC) is very common in the isolation and analysis procedures. For example, the isolation of the nonpolar bazzanins (270-288) [138,139,277], the high polar glycosides (664-667) [302] and the simultaneous determination of resveratrol derivatives and stilbeneoligomers [355-357]. Chiral column HPLC has been employed to separate the epimer mixtures of some dihydroisocoumarins [120,118] and isoplagiochin C and D type enantiomers [140,146,358]. In the isolation process of (+)-vitisin A (698), (+)-cis-vitisin A (702), (+)-hopeaphenol (716) and (-)-isohopeaphenol (717), recycled HPLC was applied [209,324]. 

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