Detection using fluorescence

A. Majumdar, and A. P. Alivisatos. Room-Temperature Single-Nucleotide Polymorphism and Multiallele DNA Detection Using Fluorescent Nanocrystals and Microarrays, Anal. Chem. 2003, 75, 4766. 

A simple method for OTC residue analysis in swine tissue was based on homogenization with both mobile phase and hexane and ultrafiltration of the supernatant with Millipore Molcut II membrane. Since TCs formed fluorescing chelates when magnesium acetate was present in the mobile phase, the chromatographic separation was detected using fluorescence detector (46). 

Klapper, L., D. M. McKnight, J. R. Fulton, E. L. Blunt-Harris, K. P. Nevin, D. R. Lovely, and P. G. Hatcher. 2002. Fulvic acid oxidation state detection using fluorescence spectroscopy. Environmental Science Technology, in press. 

For the membrane array of cytokine expression, the general procedures (Amersham Pharmacia Biotech R D Systems SuperArray Inc., Bethesda, MD Clontech Laboratories, Inc., Palo Alto, CA) include RNA extraction, reverse transcription into biotin- or radioisotope-labeled cDNA, hybridization with about 20 to several hundred different cDNA prespotted membranes, and signal detection using fluorescence or radioactive methods (L3). As an example, Fig. 4 and Table 4 show different chemokine genes upregulated in allergic asthmatic patients compared with normal controls, based on membrane array technology (SuperArray). 

Gerion, D., Chen, F.Q., Kannan, B., Fu, A.H., Parak, W.J., Chen, D.J., Majumdar, A., and Alivisatos, A.P. 2003. Room-temperature single-nucleotide polymorphism and multiallele DNA detection using fluorescent nanocrystals and microarrays. Anal Chem 75(18) 4766 1772. 

Detection using fluorescence-detected magnetic resonance (FDMR)... 

Chu L-Q, Forch R, Knoll W (2007) Surface-plasmon-enhanced fluorescence spectroscopy for DNA detection using fluorescently labeled PNA as DNA Indicator . Angew Chem Int Ed 46 4944-4947... 

Ye, L. Lucy, C. A. Chromatographic Determination of Metal-EDTA Complexes with Post Column Detection Using Fluorescent Ternary Complexes, Anal. Chem. 1995, 67, 2534-2538. 

Figure 20.6 Schematic diagram of the DNA detection using fluorescence quenching by gold nanoparticles. Oligonucleotide molecules are conjugated to gold surface vial Au-S bonds and the fluorescein molecules at the other end of the oligo sequence is adsorbed onto gold nanoparticles to form a constrained conformation.

Evans PA, Short MA, Owen RG, Jack AS, Forsyth PD, Shiach CR> et al. Residual disease detection using fluorescent polymerase chain reaction at 20 weeks of therapy predicts clinical outcome in childhood acute lymphoblastic leukemia. J Clin Oncol 1998 16 3616-27. 

To achieve increased sensitivity and reduced analysis time it is necessary to produce derivatives of the amino acids which can be separated by reversed-phase HPLC and then detected using fluorescent, spectrophotometric, or electrochemical detection. Both precolumn and postcolumn derivatization methods have been described with a range of derivat-izing agents including ... 

Biotin can be detected using fluorescent avidin, modified avidins such as UltraAvidin (Leinco Technologies) or NeutraLite Avidin (Molecular Probes), streptavidin, or antibiotin antibodies. Different secondary reagents may work better in different tissues unmodified avidin tends to result in higher background than the other choices. 

Klapper, L., McKnight, D.M., Eulton, J.R., Blunt- Harris, E.L., Nevin, K.P., Lovely, D.R., and Hatcher, P.G. (2002). Eulvic acid oxidation state detection using fluorescence spectroscopy. Environ. Sci. TechnoL, 36, 3170-3175. 

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