Reserpine tablets

Analytical methods are ripe for attack using Al methods. Capillary electrophoresis is a routine separation technique, but like other separation techniques, its effectiveness is correlated strongly with experimental conditions. Hence it is important to optimize experimental conditions to achieve the maximum degree of separation. Zhang and co-workers41 studied the separation of mixtures in reserpine tablets, in which vitamin B1 and dibazolum may be incompletely separated, as may promethazine hydrochloride and chloroquine... 

Cieri, U.R. Determination of reserpine, hydralazine HCl, and hydrochlorothiazide in tablets by liquid chromatography on a short, normal-phase column. J.AOACInt, 1994, 77, 1104-1108 [simultaneous hydralazine, reserpine tablets normal-phase fluorescence detection UV detection]... 

Hydropres-50—hydrochlorothiazide, reserpine Hydro-Seqi—hydrochlorothiazide, reserpine Hydro serpine 1 Tablets—hydroclilo rothiazide, reserpine... 

Hydroserpine 2 Tablets—hydrochbrothiazide, reserpine Hyzaar—hydrochlorothiazide, losartan potassium Inderide—hydrochlorotliiazide, propranolol HC1 Inderide LA—hydrochlorotliiazide, propranolol HC1 Lexxel Extended-Release—enalapril maleate, felodipine Lopressor—hydrochlorothiazide, metoprolol Lotensin HCT—hydrochlorothiazide, benazepril Lotrel—amlodopine, benazepril... 

Metatensin Tablets—trichlormethiazide, reserpine Minizide—polythiazide, prazosin Prinzide—hydrochlorotliiazide, lisinopril Rauzide Tablets—rauwolfia, bendroflumethiazide Regroton—chlorthalidone, reserpine Salutensin Tablets—hydroflumethiazide, reserpine Salutensin-Demi—hydrochlorothiazide, reseqiine Ser-Ap-Es—hydrochlorotliiazide, reseqiine, hydralazine HC1... 

Ancom antihypertensive compound tablets Herbbsland, Inc., Tai Chien Inc. 01/17/2003 Contains unapproved reserpine, diazepam, promethazine, and hydrochlorothiazide... 

Reserpine is extracted from powdered tablets with water saturated with ethyl acetate, after addition of a solution of propiophenone in ethyl acetate saturated with water (as internal standard). After centrifugation, the ethyl acetate layer is analysed by HPLC on a 10 pm Lichrosorb RP-8 column using methanol/0.05M sodium phosphate monobasic mixture (1 1) as the mobile phase. The flow rate is adjusted to 2 ml/minute. Detection is carried out under UV at 25U run. (Fig. 9). 

Another HPLC system to analyse reserpine and hydrochlorothiazide in two-component tablet formulations (i+0) 0.1-0.2 mg of reserpine and 25-50mg of hydrochlorothiazide in tablet form are shaken with 0.05 polythiazide solution in tetrahydrofuran (10 ml) for 20 minutes, centrifuged for five minutes at 2000 r.p.m. and 7 ml of the supernatant liquid is injected to HPLC using Lichrosorb Si 60 column and a mixture of 77 hexane, 18% isopropyl alcohol, 5 chloroform and 0.01 diethylamine as the mobile phase. 

Powdered tablets of chlorthalidone and reserpine are extracted with acetonitrile/water (9 l), centrifuged and the supernatant layer is injected to HPLC using a column of Pellamidon at 35°c and the solvent isopropanol/acetic acid/water/hexane (60 3 1 36) as the mobile phase. Detection is carried out under UV at 25b nm. 

U. R. Cieri, Determination of reserpine in tablets by liquid chromatography with fluorescence detection-revised procedure, JAOAC Int., 77 758(1994). 

U. R. Cirer, Determination of reserpine and hydrochlorothiazide in commerical tablets by LC with fluoresence and UV absorption detectors in series, JAOAC Int., 77 515 (1988). 

The presence of abnormal colours may indicate the presence of inorganic pigments (e.g. copper, nickel, or cobalt salts), dyestuffs from tablets, capsules, medicines, pesticides, and rodent baits, especially those containing warfarin, reserpine, chloralose, or diphenadione. Common rat-bait coloms are blue, green, or red and are usually associated wifti oafrneal or cereal grain. 

Cieri UR. Determination of reserpine and rescinnamine in Rauwolfia serpentina powders and tablets collaborative study. J AOAC Int 1998 81(2) 373-80. 

One problem with many of the active substances used today is their poor solubility in water and their limited bioavailability. One of the simplest means of improving the bioavailability of an active substance is to improve its dissolution by adding solubilizing agents, such as povidone. It forms water-soluble complexes with many active substances (see Sections 2.2.7 and 2.4.5). With some such substances, it may be sufficient to produce a physical mixture. Fig. 45 shows the improvement in the dissolution rate of reserpine achieved by simply mixing it with an excess of povidone K 30. For the mixture with indomethacin see Section 3.4.3.1. Similar results can be expected with the drugs listed in Section 2.4.5. That this effect also applies to finished preparations can be seen for phenytoin tablets in Fig. 52 [326]. The bioavailability of peroral gidazepam is increased by the addition of povidone too [536]. 

One of the most fascinating products containing multiple components is a combination of the natural product reserpine, hydralazine hydrochloride, and hydrochlorothiazide. This drug product is available in tablet form and contains 0.1 mg of reserpine USP, 25 mg of hydralazine hydrochloride, and 15 mg of hydrochlorothiazide (Fig. 6). Reserpine is an indole alkaloid derived from the dried root of Rauwolfia serpentina and is well known for its complex molecular architecture, the challenges faced during its total synthesis, and its profound effect on the central nervous system as an antihypertensive. Hydralazine is also an antihypertensive and hydrochlorothiazide has diuretic properties. The combination of these three very different molecular structures brings diversity to the analytical testing required. 

RESERPINE/HYDRALAZINE HYDROCHLORIDE/HYDROCHLOROTHIAZIDE (Hydrap-ES tablets 15 mg hydrochlorothiazide,... 

Hydropres-50— liydrochlorothiazide, reserpine Hydro-Seqi— liydrochlorotliiazide, r eqiine Hydroseqiine 1 Tablets— hydrochlorotliiazide, reserpine... 

Some typical results obtained for reserpine, methyl testosterone, and prednisolone with dry ACN and DMF will be given here, including composite and single tablet assays. Reserpine and methyl testosterone may be considered as representative of nearly ideal systems for analysis. Prednisolone in dry DMF and ACN shows clear evidence for a follow-up second order chemical reaction, making it a nonideal system. 

Fundamental-harmonic a.c. polarograms of a reserpine standard and a tablet extract. System (A) 1.62 x 10"5 M reserpine standard. (B) 1.65 x lO" M reserpine extracted from a tablet, at DME, 0.01 W TEAPFB-ACN interface, 2s drop life, room temperature, 25 +2 C. Applied pseudo-random, odd-harmonic a.c. waveform,... 

Fourier Transform Faradaic Admittance Measurements (FT-FAM) Table 10. Assaiy results for reserpine (0.1 mg tablet)... 

Reactive in solution Permanganate Tetrahydropalmatine (tablets) reserpine (Ph) rescinnamine (Ph) yohimbine (Ph) medazepam (Ph) cefadroxil monohydrate (Ph and BF) salicylamide (Ph and urine) perphenazine (synthetic formulations) dipyridamole (tablets and injections) etilefrine hydrochloride (Ph and BF) isoxsuprine hydrochloride (Ph and BF) prenalterol hydrochloride (Ph and BF) amidopyrine (Ph) pipemidic acid (Ph) ethamsylate (Ph) folic acid (Ph) sulphonamides (Ph) chloramphenicol (Ph)... 

---