Sodium phosphate monobasic

Fogli et al. developed and validated an HPLC method with fluorescence detection for simultaneous routine TDM of anthracyclines and their metabolites.27 They coupled a Waters LC Module I Plus system equipped with a WISP 416 autosampler with a Model 474 scanning fluorescence spectrophotometer. The stationary phase was a Supelcosil LC-CN column (250 x 4.6 mm, 5 /um particle size) with a /iBondapak-CN guard column. The mobile phase consisted of 50mM monobasic sodium phosphate buffer and acetonitrile (65 35 v/v), adjusted to pH 4.0 with phosphoric acid. The flow rate was 1 mL/min. The fluorescence detection was set at excitation wavelengths of 233, 254, and 480 nm and at an emission wavelength of 560 nm. 

Neutral buffered formalin add 100 mL of 37 0% formalin to 900 mL deionized water, then add 4 g monobasic sodium phosphate and 6.5 g dibasic sodium phosphate. Bonin s fluid Combine 1500 mL picric acid (saturated in deionized water at 21 g/L), 500 mL formalin, and 100 mL glacial acetic acid. 

Monobasic sodium phosphate, NaH2P04.H20, obtained from Merck Co., Inc., was used. This buffer was found to be satisfactory for this reaction. 

Synonyms sodium dihydrogen phosphate sodium orthophsophate, primary primary sodium phosphate sodium hiphosphate monobasic sodium phosphate... 

Monobasic sodium phosphate is used in baking powders, acid cleansers, electroplating, as a dry acidulant, and in treating boiler water. It also is a nutrient supplement in food. It is a laboratory reagent used as a buffer. 

Monobasic sodium phosphate can be prepared by partial neutralization of phosphoric acid with sodium hydroxide in equimolar amounts ... 

Per vial 23 mg glycine 1.6 mg SC dibasic sodium phosphate heptahydrate 0.55 mg monobasic sodium phosphate monohydrate... 

To be reconstituted with sterile WFI (pH approximately 7.2) Per lOmL 500 mg sucrose 0.5 mg polysorbate 80 2.2 mg monobasic sodium phosphate monohydrate 6.1 mg dibasic sodium phosphate dihydrate IV infusion... 

Per Redipen 1.013 mg dibasic sodium phosphate anhydrous 1.013 mg monobasic sodium phosphate dehydrate 54 mg sucrose 0.0675 mg polysorbate 80... 

El 1. You need to prepare a buffer for biochemistry lab. The required solution is 0.5 M sodium phosphate, pH 7.0. Use the Henderson-Hasselbalch equation to calculate the number of moles and grams of monobasic sodium phosphate (NaH2P04) and dibasic sodium phosphate (Na2HP04) necessary to make 1 liter of the solution. 

Phosphate buffer Dissolve 0.725 g of monobasic sodium phosphate and 4.472 g of anhydrous dibasic sodium phosphate in 300 ml of water, dilute with water to 1000 ml, and mix. Dilute 250 ml of this solution with water to 1000 ml. If necessary, adjust the pH with phosphoric acid to 7.6. 

Another application [12] uses a packed capillary segment combined with a bare silica section that serves to speed up the endo-osmotic flow, while the ODS-packed part of the capillary was used as the separation part of the capillary column. The detector s window was placed in-between the two segments. Monobasic sodium phosphate, 1.25 mM, pH 6.0, containing 75% v/v acetonitrile was used as background electrolyte at 625 V/cm, and 20 cm of the ODS-packed bed was used with the result... 

Polyaromatic hydrocarbons (benzene, toluene, ethylbenzene, propylbenzene, butylbenzene, pentylbenzene) Separation segment ODS, 5 pm (200 mm), accelerating segment bare silica (0-280 mm) Acetonitrile-1.25 mM monobasic sodium phosphate pH 6.0 (75 25) 100 pm i.d., for length see stationary phase Two types of packing 12... 

Indigo Reagent I Just before use, transfer 20 mL of Indigo Stock Solution, 10 g of monobasic sodium phosphate, and 7 mL of phosphoric acid into a 1-L volumetric flask, dilute to volume with water, and mix. 

Sodium Phosphate Buffer Solution Dissolve 10.4 g of monobasic sodium phosphate (NaH2P04-H20) in 500 mL of sterile, deionized water in a 1000-mL volumetric flask, and dilute to volume. Similarly, dissolve 9.465 g of anhydrous dibasic sodium phosphate (Na2HP04) in sterile, deionized water, and dilute to 1000 mL. Mix 815 mL of the monobasic sodium phosphate solution with 185 mL of the dibasic sodium phosphate solution. Adjust the pH of the buffer to 6.2 when checking the pH, use an aliquot of the buffer to prevent contamination of the solution. Adjust the pH by adding more monobasic or dibasic sodium phosphate solution as needed. The buffer solution may be stored under refrigeration for up to 1 month. 

Monobasic Sodium Phosphate is anhydrous or contains one or two molecules of water of hydration and is slightly hygroscopic. The anhydrous form occurs as a white, crystalline powder or granules. The hydrated forms occur as white or transparent crystals or granules. All forms are freely soluble in water, but are insoluble in alcohol. The pH of a 1 100 solution is between 4.1 and 4.7. 

Mixed Carbohydrase and Protease, 20 Modified Cellulose, 11 Monoammonium Phosphate, 4 Monobasic Potassium Phosphate, 40 Monobasic Sodium Phosphate, 54 Monopotassium Phosphate, 40 Monosodium Dihydrogen Phosphate, 54 Monosodium Phosphate, 54... 

Substrates (p-octopamine, dopamine, or 5-hydroxytryptamine) were separated from their N-acetylated products on an Ultrasphere I.P. C18 column (4.6 mm x 250 mm, 5 pm). The mobile phase was comprised of 75 mAf monobasic sodium phosphate, 1 pAf EDTA, 0.35 mAf 1-octanesulfonate (sodium salt), 11% methanol, and 4% acetonitrile. Coulometric detection was used. Detection of p-octopamine and its N-acetylated product was achieved at a potential of +0.75 V, while dopamine, 5-hydroxytryptamine and their products were detected at +0.50 V. 

According to the USP32 NP27 [5], mobile phase used for liquid chromatographic method consists of 0.5 g of monobasic sodium phosphate in 500 mL water which is adjusted with phosphoric acid to a pH of 2.1-2.7, and added with 500 mL of ACN. The solvent or diluents used is a mixture of ACN water = 4 1. Por the standard, USP glimepiride RS diluted in the diluents to make final concentration of 0.2 mg/mL, while the assay is prepared by dissolving 20.0 mg glimepiride assayed with the diluents to... 

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