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Using pre-columns

The most popular current techniques for amino acid analysis rely on liquid chromatography and there are two basic analytical methods. The first is based on ion-exchange chromatography with post-column derivatization. The second uses pre-column derivatization followed by reversed-phase HPLC. Derivatization is necessary because amino acids, with very few exceptions, do not absorb in the UV-visible region, nor do they possess natural fluorescence. [Pg.49]

Determination of serum cortisone by reversed-phase liquid chromatography using pre-column sulfuric acid-ethanol fluorescence derivatization and column switching. (159)... [Pg.224]

Several methods are available for laboratories to determine GA and Cr in body fluids. A sensitive HPLC method for the measurement of GA and Cr plus creatinine in biological fluids has been published by Carducci et al. [19]. The method uses pre-column derivatisation with benzoin, separation on a reverse-phase column, and... [Pg.747]

BA Bidlingmeyer, SA Cohen, TL Tarvin. Rapid analysis of amino acids using pre-column derivatization. J Chromatogr Biomed Appl 336 93-104, 1984. [Pg.94]

GP McMahon, R O Kennedy, MT Kelly. High-performance liquid chromatographic determination of taurine in human plasma using pre-column extraction and derivatization. J Pharm Biomed Anal... [Pg.95]

CR Krishnamurti, AM Heindze, G Galzy. Application of reversed-phase high-performance liquid chromatography using pre-column derivatization with o-phthalaldehyde for the quantitative analysis of amino acids in adult and fetal sheep plasma, animal feeds and tissues. J Chromatogr 315 321-331, 1984. [Pg.95]

Umagat, H., Kucera, P., and Wen, L.-F., Total amino acid analysis using pre-column fluorescence derivatization, J. Chromatogr., 239, 463, 1982. [Pg.170]

Zhou Y, Zhou J, Xu Y, Zha J, Ma M, Wang Z (2009) An alternative method for the determination of estrogens in surface water and wastewater treatment plant effluent using pre-column trimethylsilyl derivatization and gas chromatography/mass spectrometry. Environ Monit Assess 158 35-49... [Pg.283]

The rest of the examples in this report involve liquid chromatography and either pre- or post-column sample treatment. Table I will give the reader some idea of the analyses which can be done and what kinds of treatment and detectors are in use. Pre-column treatment, for example, may include cleanup by dialysis, by chromatography, by extraction, by distillation, or by precipitation of protein. Pre-column concentration may be done by chromatographic column or by evaporation. And pre-column derivatization may involve digestion or simpler reactions. At the other end of the table, post-column derivatives have been made for use with both fluorescent and colorimetric detectors. [Pg.19]

Park, S.K., Boulton, R.B., Noble A.C. (2000). Automated HPLC analysis of glutathione and thiol-containing compounds in grape juice and wine using pre-column derivatization with fluorescence detection. Food Chem., 68, 475-480. [Pg.211]

Table I presents both average error and yield data in terms of the methodology used. The accuracy of analyses performed using pre-column and post-column methods is very similar, despite the fact that pre-column users tend to analyze significantly less of the total protein than post-column users. In this year s study the top site (smallest error) was a pre-column PITC site. The second best site used post column-OPA methodology (see Table II). The average total protein yield for analyses using pre-column methods is lower than the average for postcolumn users, but the difference is not statistically significant. Both of the top two sites had higher than average yields, 7.9 and 8.4 pg, respectively. Table I presents both average error and yield data in terms of the methodology used. The accuracy of analyses performed using pre-column and post-column methods is very similar, despite the fact that pre-column users tend to analyze significantly less of the total protein than post-column users. In this year s study the top site (smallest error) was a pre-column PITC site. The second best site used post column-OPA methodology (see Table II). The average total protein yield for analyses using pre-column methods is lower than the average for postcolumn users, but the difference is not statistically significant. Both of the top two sites had higher than average yields, 7.9 and 8.4 pg, respectively.
Orford, C.D. Perry, D. Adlard, M.W. The determination of naturedly produced penicillins and their biosynthetic precursors using pre-column derivatisation with dansylaziridine. J.Liq.Chromatogr, 1991, 14, 2665-2684 [also penicillin G, penicillin K, penicillin X LOD 1000 ng/mL fluorescence... [Pg.1095]

Haginaka, J. Wakai, J. Yasuda, H. Uno, T. Nakagawa, T. High-performance liquid chromatographic assay of sulbactam using pre-column reaction with 1,2,4-triazole. J.Chromatogr., 1985, 341, 115-122... [Pg.1261]

Experiment 31. Determination of the amino acid composition of a peptide using pre-column derivatisation with o-phthalaldehyde and reverse phase HPLC and fluorescence detection Experiment 32. Analysis of inorganic anions in aqueous samples... [Pg.1]

S. A. Linde, and U. Karst. 1999. Determination of gas phase peroxyacetic acid using pre-column derivatization with organic sulfide reagents and hquid chromatography. 7. Chromatography A 855(2) 551-61. [Pg.133]

Kim B-H, Lee SC, Lee HJ, Ok JH, reversed phase liquid chromatographic method for the analysis of aminoglycoside antibiotics using pre-column derivatization with phenyliso-cyanate, Biomed. Chromatogr. 2003 17 396-403. [Pg.261]

Solvent loss by evaporation can very easily lead to incorrect results and must be continually checked. The most convenient way to do this is to determine periodically the activity coefficient of a particular solute chosen for its convenience. For some solvents it is necessary to use pre-columns or pre-saturators to saturate the carrier gas with solvent upstream of the column. This has been done by many workers and is essential if the range of systems to be studied by the g.I.c. method is to be extended. The loss of solvent has been determined by the direct weighing of the column but this has obvious drawbacks. [Pg.54]

Cohen SA (2003) Amino acid analysis using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccini-midyl carbamate. Analysis of hydrolyzed proteins and electrohlotted samples. Methods in Molecular Biology 211 143-154. [Pg.3565]

K. Shimada, M. Tanaka and T. Nambara, Studies on steroids. CC. Determination of 17-ketosteroid sulphates in serum by high-performance liquid chromatography with electrochemical detection using pre-column derivatization, J. Chromatogr., 1984, 307, 23-28. [Pg.77]

Eigure 7A illustrates successful detection of four neuropeptides at 100 pM to 1 nM using pre-column biuret derivatization, on-column preconcentration, and... [Pg.504]

Okayama, A. Fujii, S. Miura, R. Optimized fluorometric determination of urinary delta-aminolevulinic add by using pre-column derivatization, and identification of the derivative, Clin.Chem., 1990, 36, 1494-1497. [Pg.35]

Li, J. Milne, R.W. Nation, R.L. Tumidge, J.D. Coulthard, K. Johnson, D.W. A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography, J.Chromatogr.B, 2001, 761, 167-175. [Pg.153]

See other pages where Using pre-columns is mentioned: [Pg.1227]    [Pg.1077]    [Pg.68]    [Pg.80]    [Pg.1227]    [Pg.407]    [Pg.275]    [Pg.499]    [Pg.218]   
See also in sourсe #XX -- [ Pg.161 ]



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Column Use

Pre-column

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