Quantitation high-performance liquid chromatography

Frenkel, K., Zhong, Z., Wei, H., Karkoszka, J., Patel, U., Rashid, K., Georgescu, M. and Solomon, J.J. (1991). Quantitative high-performance liquid chromatography analysis of DNA oxidized in vitro and in vivo. Anal. Biochem. 196, 126-136. 

Baker et al. [138] studied the excretion of metabolites in bile following the administration of primaquine in rats. Six metabolites of primaquine were found in the bile of rats. Quantitative high performance liquid chromatography analysis of the metabolites revealed that the sum of the six metabolites excreted in the bile represented quantitative recovery of the dose of primaquine. 

Taylor, P. J. Matrix effects the Achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry. Clin Biochem 2005, 38, 328-334. 

F. Y. L. Hsieh et al.. Kinetic monitoring of enz3mic reactions in real time by quantitative high-performance liquid chromatography-mass spectrometry. Anal. Biochem. 229, 20-25 (1995). 

Li H, Lawson JA, Reilly M, Adiyaman M, Hwang SW, Rokach J, FitzGerald GA. Quantitative high performance liquid chromatography/tandem mass spectrometric analysis of the four classes of F(2)-isoprostanes in human urine. Proc Natl Acad Sci USA 1999 96 13381-13386. 

Hsieh, F.Y., Tong, X., Wachs, T, Ganem, B., Henion, J. (1995) Kinetic Monitoring of Enzymatic Reactions in Real Time by Quantitative High-performance Liquid Chromatography-Mass Spectrometry. Anal. Biochem. 229 20-25. 

In this context, it is of interest to consider recent results of a quantitative high-performance liquid chromatography analysis of lipids from the deepest part of the stratum corneum, the stratum compactum. This study shows that the lipid yield after stripping of the skin in vivo (Table 1) results in a composition... 

Yang, P, Felix, E, Madden, T, Fischer, SM and Newman, RA (2002) Quantitative high-performance liquid chromatography/electrospray ionization tandem mass spectrometric analysis of 2- and 3-series prostaglandins in cultured tumor cells. Anal Biochem, 308,168-177. 

Jacobsen, P.B. On-line dialysis and quantitative high-performance liquid chromatography analysis of iodixanol in human, rat and monkey plasma, J.Chromatogr.B, 2000, 749, 135-142. 

Malayappan, B. et (2010) Quantitative high-performance liquid chromatography-electrospray ionization tandem mass spectrometry analysis of bis-N7-guanine DNA-DNA cross-links in white blood cells of cancer patients receiving cyclophosphamide therapy. Anal. Chem., 82 (9), 3650-3658. 

Typically, quantitative protein determination is done on the one hand by colorimetric or nephelometric methods, on the other hand for more difficult analytical problems by more sophisticated techniques such as high performance liquid chromatography (HPLC), gel-electrophoresis and immunoassay. However, these methods are tedious, time-consuming and expensive. 

Chromatography is a separation process employed for the separation of mixtures of substances. It is widely used for the identification of the components of mixtures, but as explained in Chapters 8 and 9, it is often possible to use the procedure to make quantitative determinations, particularly when using Gas Chromatography (GC) and High Performance Liquid Chromatography (HPLC). 

High performance liquid chromatography is used for the separation and quantitative analysis of a wide variety of mixtures, especially those in which the components are insufficiently volatile and/or thermally stable to be separated by gas chromatography. This is illustrated by the following method which may be used for the quantitative determination of aspirin and caffeine in the common analgesic tablets, using phenacetin as internal standard where APC tablets are available the phenacetin can also be determined by this procedure. 

Quantitation Using High Performance Liquid Chromatography 23... 

Quantitation in high performance liquid chromatography, as with other analytical techniques, involves the comparison of the intensity of response from an analyte ( peak height or area) in the sample under investigation with the intensity of response from known amounts of the analyte in standards measured under identical experimental conditions. 

In a study of the metabolism of methyl parathion in intact and subcellular fractions of isolated rat hepatocytes, a high performance liquid chromatography (HPLC) method has been developed that separates and quantitates methyl parathion and six of its hepatic biotransformation products (Anderson et al. 1992). The six biotransformation products identified are methyl paraoxon, desmethyl parathion, desmethyl paraoxon, 4-nitrophenol, />nitrophenyl glucuronide, and /wiitrophenyl sulfate. This method is not an EPA or other standardized method, and thus it has not been included in Table 7-1. 

JUSTESEN u, KNUTHSEN p and LETH T (1998) Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photo-diode array and mass specfrometric detection, /C/u matogr A, 799, 101-10. 

Heath, D.D. et ah, Curcumin in plasma and urine quantitation by high-performance liquid chromatography, J. Chromatogr. BAnalyt. Technol. Biomed. Life ScL,7S3, 287, 2003. 

Saito, K., A new enzymatic method for extraction of precarthamin from dyer s saffron (Carthamus tinctorius) florets, Z. Lebensmitt. Untersuch. Forsch., 197, 34, 1993. Cserhati, T. et ah. Separation and quantitation of colour pigments of chili powder (Capsicum frutescens) by high-performance liquid chromatography-diode array detection, J. Chromatogr. A, 896, 69, 2000. 

Gregory, G.K. et ah. Quantitative analysis of lutein esters in marigold flowers (Tagetes erecta) by high performance liquid chromatography, J. Food ScL, 51, 1093, 1986. Livingston, A.L., Rapid analysis of xanthophyll and carotene in dried plant materials, J. AOAC, 69, 1017, 1986. 

The octanol-water parhtion coefficient. Poet (often reported as log Poet), is a particularly useful parameter in quantitative structure-achvity relationships, apphed to predichon of properhes related to drug absorphon, distribution, metabohsm and excrehon [61, 62]. Although the traditional log Poet measurements have been done by the shake-flask method [63, 64], high-performance liquid chromatography-... 

Kaliszan, R., Baczek, T., Cimochowska, A., Juszczyk, P., Wisniewska, K., Grzonka, Z. Prediction of high-performance liquid chromatography retention of peptides with the use of quantitative structure-retention relatiorrships. Proteomics 2005, 5, 409 15. 

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