Chromatography, quantitative

In report separately discuss the peculiarities of determination of the anion composition of the solid solutions, that conditioned by ability of diphosphate anion to destruction in water solutions. In given concrete case by most acceptable method of control of the diphosphate anion in the hydrated solid solutions is a traditional method of the quantitative chromatography on the paper. Methodical ways which providing of minimum destruction of the diphosphate anion in the time of preparation of the model to analysis (translation in soluble condition) and during quantitative determination of the P.,0, anion are considered. 

Separation and purification chromatography has to be applied before the quantitative chromatography. Some purification techniques applied are lEC, such as DEAE-Sephadex A-25 [571], weak anion-exchange column [572,573], strong anion-exchange column (564), cation-exchange column... 

Because they are important, the phenolics and their roles in wine have been studied considerably (I), but more needs to be known. Since different classes of phenols behave quite differently in the various roles as pigments, oxidation substrates, flavors, etc., separate consideration and analysis is essential to completely understand them. Every individual phenol should be separately determined precisely and quantitatively. Chromatography may solve these problems, but for now, chromatography and other detailed separatory methods have drawbacks, particularly for economical application to many individual wine or grape samples. 

W.A.Schroeder, AnnNYAcadSci 49, 204-17 (1948) (Systematic quantitative chromatography as applied to analysis of expls proplnts) 26)F.Pristera, PATR 1691... 

An example of the use of an internal standard is the precision enhancement for univariate quantitative chromatography based on peak area. With manual sample injection, the reproducibility of volume size is not consistent. For this approach to be successful, the internal standard peak must be well separated from the peaks of any other sample constituent. Use of an internal standard can be avoided with an autosampler. 

Hejtmanek, M. Quantitative chromatography of inorganic substances, II. Chromatographic microdetermination of nickel, cobalt and copper by photometry in situ. Sb. Vysoke Skoly Chem.-Technol. v Praze, Oddil Fak. Anorg. Org. Technol. 4, 69 (1960) C. A. 67, 1 247 a (1964)... 

L3. Lewis, B., Preparation of paper for quantitative chromatography of corticosteroids. Bioohim. Biophys. Acta 20, 396 (1956). 

The range of standards for quantitative chromatography was determined by the response observed for the samples to be assayed. Standards and samples were intermixed on the chromatography sheets. Zone diameters were read to the nearest millimeter and these values were used to construct the regression lines for estimation of antibiotic concentrations. Appropriate controls were included in these runs... 

Quantitative Methods used in quantitative chromatography are decribed in Topic D2 and... 

For CE and CEC, the methods used for quantitative chromatography (Topic D2) are suitable, and alternative calibration procedures are described in Topic A5. Peak areas are more reliable than peak heights, as they are directly proportional to the quantity of a solute injected when working within the linear range of the detector. 

---