High-performance liquid chromatography quantitative

C Garcia, L Marina, M Torre. Simultaneous separation of soya bean and animal whey proteins by re-versed-phase high-performance liquid chromatography. Quantitative analysis in edible samples. Anal Chem 69 2217-2220, 1997. 

Georga, K.A. Samanidou, V.F. Papadoyannis, I.N. Use of novel solid-phase extraction sorbent materials for high-performance liquid chromatography quantitation of caffeine metabolism products methylxanthines and methyluric acids in samples of biological origin. J. Chromatogr., B 2001, 759, 209 -218. 

Weber, A. Opheim, K.E. Siber, G.R. Ericson, J.F. Smith, A.L. High-performance liquid chromatography quantitation of trimethoprim, sulfamethoxazole, and N4-acetylsulfamethoxazole in body fluids. J.Chromatogr., 1983, 278, 337—345... 

Altomare C, Caroiti A. Cellamare S, Cameri A. Ciabatti R, Malabarba A, Newman DJ. Oh YK, Phelen C. Shearer MC, Sitrin RD, Nisbet LJ. Lipophilicicy of teicoplanin antibiotics as assessed by reversed phase high-performance liquid chromatography Quantitative structure-property and structure-activity relationships. J Pharm Pharmacol 1994 46 994-999. 

Erni, E, Frei, R.W. and Lindei W. (1976) A low-cost gradient system for high-performance liquid chromatography. Quantitation of complex pharmaceutical raw materials. J. Chromatogr., 125,265-274. 

Typically, quantitative protein determination is done on the one hand by colorimetric or nephelometric methods, on the other hand for more difficult analytical problems by more sophisticated techniques such as high performance liquid chromatography (HPLC), gel-electrophoresis and immunoassay. However, these methods are tedious, time-consuming and expensive. 

Chromatography is a separation process employed for the separation of mixtures of substances. It is widely used for the identification of the components of mixtures, but as explained in Chapters 8 and 9, it is often possible to use the procedure to make quantitative determinations, particularly when using Gas Chromatography (GC) and High Performance Liquid Chromatography (HPLC). 

High performance liquid chromatography is used for the separation and quantitative analysis of a wide variety of mixtures, especially those in which the components are insufficiently volatile and/or thermally stable to be separated by gas chromatography. This is illustrated by the following method which may be used for the quantitative determination of aspirin and caffeine in the common analgesic tablets, using phenacetin as internal standard where APC tablets are available the phenacetin can also be determined by this procedure. 

Quantitation Using High Performance Liquid Chromatography 23... 

Quantitation in high performance liquid chromatography, as with other analytical techniques, involves the comparison of the intensity of response from an analyte ( peak height or area) in the sample under investigation with the intensity of response from known amounts of the analyte in standards measured under identical experimental conditions. 

In a study of the metabolism of methyl parathion in intact and subcellular fractions of isolated rat hepatocytes, a high performance liquid chromatography (HPLC) method has been developed that separates and quantitates methyl parathion and six of its hepatic biotransformation products (Anderson et al. 1992). The six biotransformation products identified are methyl paraoxon, desmethyl parathion, desmethyl paraoxon, 4-nitrophenol, />nitrophenyl glucuronide, and /wiitrophenyl sulfate. This method is not an EPA or other standardized method, and thus it has not been included in Table 7-1. 

JUSTESEN u, KNUTHSEN p and LETH T (1998) Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photo-diode array and mass specfrometric detection, /C/u matogr A, 799, 101-10. 

Heath, D.D. et ah, Curcumin in plasma and urine quantitation by high-performance liquid chromatography, J. Chromatogr. BAnalyt. Technol. Biomed. Life ScL,7S3, 287, 2003. 

Saito, K., A new enzymatic method for extraction of precarthamin from dyer s saffron (Carthamus tinctorius) florets, Z. Lebensmitt. Untersuch. Forsch., 197, 34, 1993. Cserhati, T. et ah. Separation and quantitation of colour pigments of chili powder (Capsicum frutescens) by high-performance liquid chromatography-diode array detection, J. Chromatogr. A, 896, 69, 2000. 

Gregory, G.K. et ah. Quantitative analysis of lutein esters in marigold flowers (Tagetes erecta) by high performance liquid chromatography, J. Food ScL, 51, 1093, 1986. Livingston, A.L., Rapid analysis of xanthophyll and carotene in dried plant materials, J. AOAC, 69, 1017, 1986. 

The octanol-water parhtion coefficient. Poet (often reported as log Poet), is a particularly useful parameter in quantitative structure-achvity relationships, apphed to predichon of properhes related to drug absorphon, distribution, metabohsm and excrehon [61, 62]. Although the traditional log Poet measurements have been done by the shake-flask method [63, 64], high-performance liquid chromatography-... 

Kaliszan, R., Baczek, T., Cimochowska, A., Juszczyk, P., Wisniewska, K., Grzonka, Z. Prediction of high-performance liquid chromatography retention of peptides with the use of quantitative structure-retention relatiorrships. Proteomics 2005, 5, 409 15. 

Frenkel, K., Zhong, Z., Wei, H., Karkoszka, J., Patel, U., Rashid, K., Georgescu, M. and Solomon, J.J. (1991). Quantitative high-performance liquid chromatography analysis of DNA oxidized in vitro and in vivo. Anal. Biochem. 196, 126-136. 

Residue analytical methods for neonicotinoids in crops, soil and water samples have been developed. The basic principle of these methods consists of the following steps extraction of the crop and/or soil samples with acetone or the other organic solvent, cleanup by liquid-liquid partition or column chromatography, and quantitative analysis by high-performance liquid chromatography with ultraviolet detection (HPLC/UV). Simple column cleanup procedures are used to improve the accuracy and sensitivity of these methods. 

Milbemectin consists of two active ingredients, M.A3 and M.A4. Milbemectin is extracted from plant materials and soils with methanol-water (7 3, v/v). After centrifugation, the extracts obtained are diluted to volume with the extraction solvent in a volumetric flask. Aliquots of the extracts are transferred on to a previously conditioned Cl8 solid-phase extraction (SPE) column. Milbemectin is eluted with methanol after washing the column with aqueous methanol. The eluate is evaporated to dryness and the residual milbemectin is converted to fluorescent anhydride derivatives after treatment with trifluoroacetic anhydride in 0.5 M triethylamine in benzene solution. The anhydride derivatives of M.A3 and M.A4 possess fluorescent sensitivity. The derivatized samples are dissolved in methanol and injected into a high-performance liquid chromatography (HPLC) system equipped with a fluorescence detector for quantitative determination. 

Previati, M., Bertolaso, L., Tramarin, M., Bertagnolo, V., and Capitani, S., Low nanogram range quantitation of diglycerides and ceramide by high-performance liquid chromatography, Anal. Biochem., 233, 108, 1996. 

Kaliszan, R., Quantitative structure-retention relationships applied to reversed-phase high-performance liquid chromatography, /. Chromatogr. A, 656, 417, 1993. 

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