Transgenic animals recombinant proteins

Fig. 8.10 Titers of antibodies at day 50 induced by plant-derived CTB-2L21 recombinant protein. Balb/c mice were intraperitoneally immunized with leaf extract from CTB-2L21 transgenic plants. Animals were boosted at days 21 and 35. Each mouse received 20 pg of CTB-2L21 recombinant protein. Individual samples of mouse serum were titrated against 2L21 synthetic peptide,VP2 protein and a control peptide (amino acids 122-135 of hepatitis B virus surface antigen). Titers were expressed as the highest serum dilution to yield twice the absorbance mean of preimmune sera. M1-M6 mice 1 to 6 2L21 epitope from the VP2 protein of the canine parvovirus CTB cholera toxin B VP2 protein of the canine parvovirus that includes the 2L21 epitope.

The development of a by-production system could further minimize the cost of such plant-derived products. For example, potatoes are the raw material for the production of technical grade starch. During this process, soluble proteins are separated by heat treatment and sold as animal fodder. Recombinant proteins could be produced in transgenic potato tubers as a by-product of starch extraction, and this would be useful for proteins produced in large amounts with a low commercial impact, like structural fiber proteins. 

In addition to milk, a range of recombinant proteins have been expressed in various other targeted tissues/fluids of transgenic animals. Antibodies and other proteins have been produced in the blood of transgenic pigs and rabbits. This mode of production, however, is unlikely to be pursued industrially for a number of reasons ... 

Therapeutic proteins have also been successfully expressed in the urine and seminal fluid of various transgenic animals. Again, issues of sample collection, volume of collected fluid and the appropriateness of these systems render unlikely their industrial-scale adoption. One system that does show industrial promise, however, is the targeted production of recombinant proteins in the egg white of transgenic birds. Targeted production is achieved by choice of an appropriate... 

Recombinant proteins, 11 23—24 clinical use of, 12 520 production in transgenic farm animals, 12 4644 65... 

Uses of cloned genes Produce recombinant proteins Gene therapy (somatic) Transgenic animals (germ line) Produce cDNA probes for blots... 

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