Recombinant protein Escherichia coli

J. S. Pati ick and A. L. Lagu, Determination of recombinant human proinsulin fusion protein produced in Escherichia coli using oxidative sulfitolysis and two-dimensional HPLC, Chem. 64 507-511 (1992). 

Shimomura, O., and Inouye, S. (1999). The in situ regeneration and extraction of recombinant aequorin from Escherichia coli cells and the purification of extracted aequorin. Protein Expression and Purification 16 91-95. 

Recombinant DNA technology can also be used to design genes that encode for proteins with desired features [34]. The gene can be incorporated into a plasmid, which is then used to transform a bacterial host such as Escherichia coli. Finally, the production of the desired amino acid polymer is performed by the host with a precisely defined sequence and near absolute monodispersity [29, 35]. 

Goodacre, R. Karim, A. Kaderbhai, M. A. Kell, D. B. Rapid and quantitative analysis of recombinant protein expression using pyrolysis mass spectrometry and artificial neural networks Application to mammalian cytochrome b5 in Escherichia coli. J. Biotechnol. 1994,34,185-193. 

Sorensen, H.P. and Mortensen, K.K. (2005) Advanced genetic strategies for recombinant protein expression in Escherichia coli. Journal of Biotechnology, 115, 113-128. 

Induced mutagenesis in Escherichia coli is an active process involving proteins with DNA replication, repair, and recombination functions. The available evidence suggests that mutations are generated at sites where DNA has been damaged and that they arise via an error-prone repair activity. In an attempt to understand what specific contributions to mutagenesis are made by DNA lesions, we have studied the mutational specificity of some carcinogens, such as benzo[a]pyrene and aflatoxin, whose chemical reactions with DNA are... 

Mergulhao, F.J.M., Monteiro, G.A., Cabral, J.M.S., and Taipa, M.A. 2004. Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli. Journal of Microbiology and Biotechnology. 14, 1-14. 

Schumann, W. and Ferreira, L. 2004. Production of recombinant protein in Escherichia coli. Genetics and Molecular Biology. 27,442-453. 

Escherichia coli as a source of recombinant, therapeutic proteins... 

The concept of recombinant DNA technology is based on the premise that a gene sentence may be taken from an animal or human gene responsible for the production of a particular protein and inserted into the DNA of Escherichia coli, a single-cell bacterium. The bacterial cells then divide very rapidly, making billions of copies of themselves, including a rephea of the gene that has been inserted. 

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