Purity requirements, recombinant proteins

Protein Purity. During the production of recombinant proteins, process monitoring is required to assure purity levels required in every step. The recovery and purification of product from fermentation broth typically involve various procedures, such as filtration, centrifugation, and chromatography. After each purification and final step, constituent levels must be determined to ensure that the desired levels of purity have been achieved. In addition to its control function, this purity information is also frequently used to further optimize purification processes. CZE and SDS-CGE can be mostly used for the purity checks of protein products. 

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