Recombinant proteins, macromolecular

The events leading to the macromolecular associations observed with (3-lactoglobulin appear to be mediated by disulfide interactions. The initial solution proposed to reduce thermoinduced aggregation was to remove the Cys 121 and replace it with alanine (Cho et al., 1994). Unfortunately, recombinant protein could not be purified from inclusion bodies. The alternative... 

Protein polymers based on Lys-25 were prepared by recombinant DNA (rDNA) technology and bacterial protein expression. The main advantage of this approach is the ability to directly produce high molecular weight polypeptides of exact amino acid sequence with high fidelity as required for this investigation. In contrast to conventional polymer synthesis, protein biosynthesis proceeds with near-absolute control of macromolecular architecture, i.e., size, composition, sequence, topology, and stereochemistry. Biosynthetic polyfa-amino acids) can be considered as model uniform polymers and may possess unique structures and, hence, materials properties, as a consequence of their sequence specificity [11]. Protein biosynthesis affords an opportunity to completely specify the primary structure of the polypeptide repeat and analyze the effect of sequence and structural uniformity on the properties of the protein network. 

Derewenda, Z. S. 2004. The use of recombinant methods and molecular engineering in protein crystallization. Methods A Companion to Methods in Enzymology Macromolecular Crystallization (ed. A. McPherson) 34(3) 354—363. 

In this chapter, we review recombinant methods for engineering artifidal protdns, the range of macromolecular structures that have been prepared using these methods, and the ways in which these materials have been exploited in a variety of applications. We will discuss the design and synthesis of biologically inspired protein polymers as well as the de novo design of nonnatural amino acid sequences for the s)mthesis of protdn polymers with unique and useful properties. Several other chapters in this volume discuss in depth many of the most commonly investigated stmctural proteins and the... 

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