Recombinant green fluorescent protein

Deschamps, J. R., Miller, C. E., and Ward, K. B. (1995). Rapid purification of recombinant green fluorescent protein using the hydrophobic properties of an HPLC size-exclusion column. Protein Expression and Purification 6 555-558. 

Terry, B. R., Matthews, E. K., and Haseloff, J. (1995). Molecular characterization of recombinant green fluorescent protein by fluorescent correlation microscopy. Biochem. Biophys. Res. Commun. 217 21—27. 

Richards HA, Han CT, Hopkins RG et al (2003) Safety assessment of recombinant green fluorescent protein orally administered to weaned rats. J Nutr 133 1909-1912... 

Evaluation of Recombinant Green Fluorescent Protein, Under Various Culture Conditions and Purification with HiTrap Hydrophobic Interaction Chromatography Resins... 

Index Entries Recombinant green fluorescent protein GFPuv hydro-phobic interaction chromatography sodium dodecylsulfate polyacrylamide gel electrophoresis three-phase partitioning extraction. 

The relationship between chitinolytic gene expression and extracellular chitinase activity in individual cells of the marine bacterium Pseudoalteromonas s, strain S91 attached to solid chitin were evaluated likewise, the mutation of active site residues in the chitin-binding domain ChBD(ChiAl) of chitinase Al of Bacillus circulans was found to alter the substrate specificity (Baty et al., 2000 Hardt Laine, 2004). Escherichia co7 expressing recombinant green fluorescent protein was used to test the bactericidal efficacy of a newly synthesized chitosan-Ag nanoparticles that had significantly higher antimicrobial activity than the components at their respective concentrations (Sanpui et al., 2008). 

Quantum yield of luciferin. Various values of quantum yield have been reported for coelenterazine in the luminescence reaction catalyzed by Renilla luciferase 0.055 (Matthews et al., 1977a), 0.07 (Hart, et al., 1979), and 0.10-0.11 (with a recombinant form Inouye and Shimomura, 1997). The quantum yield is significantly increased in the presence of Renilla green fluorescent protein (GFP) see below. 

Gonzalez, D. S., Sawyer, A., and Ward, W. W. (1997). Spectral perturbations of mutants of recombinant Aequorea victoria green-fluorescent protein (GFP). Photochem. Photobiol. 65 21S. 

Inouye, S., and Tsuji, F. I. (1994a). Aequorea green fluorescence protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341 277-280. 

The use of recombinant inhibitors becomes easy when the protein is attached to green fluorescent protein (GFP), which eases the interpretation of the data [e.g., in the case of the Epsl5 mutant GFP-EA95/295 (epidermal growth factor receptor (EGFR) pathway substrate clone 15 mutant), a regulatory protein of clathrin assembly (57,58)]. 

Kawamoto, S., Niwa, H., Tashiro, F., Sano, S., Kondoh, G., Takeda, J. et al. (2000) A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination. FEBS Lett., 470, 263-268. 

Also, the Phe-64 -> Leu and Ser-65 —> Thr mutant shows higher fluorescence parameters than that of the wild GFP. Excitation at 458 nm yields a fluorescence spectrum with two peaks at 512 and 530 nm. The fluorescence properties of this enhanced green fluorescent protein (EGFP) were found to be similar to the recombinant glutathione S-transferase-EGFP (GST-EGFP) protein, expressed in Escherichia coli (Cinelli etal. 2004). 

Imaging assays (DiscoveRx PathHunter, Bioimage) Can use recombinant green fluorescent or yellow fluorescent fusion proteins to measure kinase-stimulated signalling events such as protein translocation to nucleus or membrane to cytosolic translocations Biological assay can present kinase in native form examines functional consequences of test compound Difficult to develop lower throughput Nickischer (2006) Traskjr (2006)... 

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