Recombinant proteins, purification

Wu WY, Fong BA, Gilles AG, Wood DW (2009) Recombinant protein purification by selfcleaving elastin-like polypeptide fusion tag. Curr Protoc Prot Sci 26.4 21-18... 

Londo, T., Lynch, R, Kehoe, T., Meys, M., and Gordon, N., Accelerated recombinant protein purification process development. Automated, robotics-based intergration of chromatographic purification and analysis, /. Chromatogr. A, 798, 73, 1998. 

Infant formula, tocopherol/tocotrienol analysis, 479, 487, 489 (table) Infrared spectroscopy, see also Fourier transform infrared spectroscopy fat measurement, 572 trans fatty acids, 505 Infrared/ultrasonic scanner for emulsion creaming, 597-598 Injection techniques for GC, 449 Insoluble recombinant proteins, purification of, 276... 

Menkhaus, T.J. Pate, C. Krech, A. Glatz, C.E. Recombinant protein purification from pea. Bio-technol. Bioeng. 2004, 86, 108-114. 

Palani, S., Gueorguieva, L., Rinas, U., Seidel-Morgenstern, A., and Jayaraman, G. (2011) Recombinant protein purification using gradient-assisted simulated moving bed hydrophobic interaction chromatography, part I selection of chromatographic system and estimation of adsorption isotherm. 

Recombinant Protein Purification 9. Conclusion and Perspectives References... 

In addition to the commonly used synthetic polymers such as pNIPAM and poly(N-vinylcaprolactam) (PVCL), elastin-like polymers were introduced as thermoresponsive polymers for affinity precipitation (Shimazu et al., 2003). An elastin-like polymer consists of the repeating penta-peptide, Val-Pro-Gly-Xaa-Gly (Xaa being any amino acid except proline). It undergoes reversible phase transition under temperature or salt concentration changes similar to pNIPAM (Shimazu et a/., 2003). An elastin-like polymer was used as a terminal tag for the facilitation of recombinant protein purification as attaching it to the hydrolase enzyme improves its purification. Purification, in excess of 1300-fold was achieved after two cycles of reversible changes of elastin-like polypeptide conformation precipitation induced by environmental changes (Shimazu et al., 2003). 

Several works describe the use of CBMs as a tag for recombinant protein purification [24, 173-178] and enzyme immobilization [179-181]. Depending on the binding reversibility. 

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