Molecular recombinant proteins

Whereas recombinant proteins produced as inclusion bodies in bacterial fermentations may be amenable to reversed-phase chromatography (42), the use of reversed-phase process chromatography does not appear to be widespread for higher molecular weight proteins. 

Brooks, S.A. (2004) Appropriate glycosylation of recombinant proteins for human use implications of choice of expression system. Molecular Biotechnology, 28 (3), 241-255. 

M.-A. DAoust, U. Busse, M. Martel et al., Alfalfa An efficient bioreactor for continuous recombinant protein production, in Molecular Farming of Plants and Animals for Human and Veterinary Medicine. 2003, eds. L. Erickson,... 

When sufficiently high levels of expression and protein accumulation are achieved, efficient downstream processing protocols must be developed to insure product quality and the economic feasibility of production. As the demand for safe, recombinant pharmaceutical proteins continues to expand, the market potential of plant-produced recombinant proteins is considerable. Molecular farming can produce recombinant proteins at a lower cost than traditional expression systems based on microbial or animal cell culture, and without the risk of contamination with human pathogens. 

In order to make molecular farming commercially profitable, recombinant proteins must be produced at a sufficiently high yield and in an active form. It has become clear that, for high-level protein accumulation, the stability of transgene expression can be as important as the expression level itself. The quantity of protein is determined by the rate of protein synthesis, assembly as well as proteolytic degradation [83]. 

Molecular Farming Plants as Biofactories for the Production of Recombinant Proteins... 

Recombinant proteins with unique properties can potentially generate new markets and penetrate into existing markets if they can be supplied on a large scale. An ideal system would produce the safest biologically active material at the lowest cost, and would be used in combination with an inexpensive and simple purification process. So far, there have been several examples of the high-yield production of recombinant proteins in transgenic crop plants, mainly in the area of molecular medicines such as antibodies, enzymes and vaccines [45, 48-50]. Modern agricultural practices offer... 

This chapter provides an overview of the different expression hosts, systems and strategies available in molecular farming, and discusses their advantages and disadvantages for the production of different types of recombinant proteins. 

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