Protein recombination synthetic

Flavor notes and products of lipid oxidation in cooked meats, 85,86/ Flavor peptides convenient synthesis, 149-156 enzymatic synthesis, 151,154-156 protein recombination synthetic method, 149... 

Today, it is well-known that peptides or proteins exhibit various kinds of taste. Our group has been researching on the relationship between taste and structure of peptides, BPIa (Bitter peptide la, Arg-Gly-Pro-Pro-Phe-Ile-Val) (7 as a bitter peptide, Om-p-Ala-HCl (OBA), Om-Tau-HCl as salty peptides(2j, and "Inverted-Aspartame-Type Sweetener" (Ac-Phe-Lys-OH) as a sweet peptide(5). The relationship between taste and chemical structure was partly made clear. Since commercial demand for these flavor peptides is increasing, we need to develop new synthetic methods which can prepare these peptides in large scale. We developed the following two methods (1) protein recombination method as a chemical method, (2) enzymatic synthesis using chemically modified enzyme as a biochemical method. 

To proceed further with structural studies of IFN- , access to larger quantities of pure protein was required. The obvious route to this end entailed production of recombinant protein. A synthetic gene for IFN- was designed... 

Derivation Hydrolysis of protein, also synthetically and by recombinant DNA techniques. 

Figure 13 Semisynthesis of homogeneous glycosylated GlyCAM-1 by expression protein ligation (EPL) combined with native chemical ligation (NCL) of recombinant protein and synthetic glycopeptide fragments.

Stevens PW, Raffen R, Hanson DK, Deng YL, Berrios Hammond M, Westholm FA, Murphy C, Eulitz M, Wetzel R, Solomon A, et al. Recombinant immunoglobulin variable domains generated from synthetic genes provide a system for in vitro characterization of light-chain amyloid proteins. Protein Sci 1995 4 421-432. 

Ness, J.E., Kim, S., Gottman, A. etal. (2002) Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently. Nature Biotechnology, 20, 1251-1255. 

As an alternative to purely synthetic injectable materials, an NPR system composed of a recombinant protein block copolymer of silk and elastin has been... 

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