Polyclonal antibodies adjuvant

Polyclonal Antibodies against FORL r purified polygalacturonase were raised in white rabbits. For the first immunization 200 jxg of purified protein in 300 jxl of distilled water was mixed with 200 1 of PBS and 500 n of complete Freund s adjuvcmt and injected intramusculary into the leg. Two subsequent intramuscular injections, each containing 300 fig of protein in 1 ml of incomplete Freund s adjuvant were given at 1 month intervals. Finally, the rabbit was bled 1 week later. The antisera, separated from blood by incubation at 37 "C, were stored in 1 ml fractions at -20 C. 

Barnes DA, Tse J, Kaufhold M, et al. Polyclonal antibody directed against human RANTES ameliorates disease in the Lewis rat adjuvant-induced arthritis model. J Clin Invest 1998 101(12) 2910-2919. 

Polyclonal antibody preparations have been used to induce passive immunity against a range of foreign (harmful) agents, and vaccines are used efficiently, and safely, to promote active immunization. Adjuvants are usually co-administered with the vaccine preparation, in order to enhance the immune response against the vaccine. 

Polyclonal antibodies are produced by injecting an antigen into an animal in the presence of an adjuvant containing bacterial lipopolysaccharides that stimulate the immune system. Serum prepared from the blood contains several different classes of antibodies that interact with different domains in the antigen molecule, each of... 

The immunisation procedure (at least 12 weeks with booster injections, addition of oil based adjuvants to stimulate the immunogenic response) gives rise to an antiserum representing a mixture of polyclonal antibodies which are produced by different immunocompetent cells and thus have different binding sites and affinities. 

R. S., Synthetic peptide vaccine development measurement of polyclonal antibody affinity and cross-reactivity using a new peptide capture and release system for surface plasmon resonance spectroscopy, J. Mol. Recog. 17, 540-557, 2004 Stills, H.F, Jr., Adjuvants and antibody production dispelling the myths associated with Freund s complete and other adjuvants, ILAR J. 46, 280-293, 2005 Miller, L.H., Saul, A., and Mahanty,... 

The polyclonal antibodies obtained by immunizing experimental animals have been and will continue to be satisfactory reagents for many immunoassay methods. Choices can be made among adjuvants, routes of injection, dosage and immunization schedules, species of animal to be immunized, etc. (2-6, 28-31). 

Standard immunizations of mouse involve two injections, or boosts , of a hapten-carrier protein conjugate together with an adjuvant at a 15 days interval. At least eight weeks after the second boost, immune cells are stimulated by a final intravenous injection of hapten-conjugate, and the fusion carried out 4 days later to generate hybridoma from spleen cells (Fig. 7). Experiments with catalytic polyclonal antibodies [103] have shown that over-immunization re-... 

For the generation of polyclonal antibodies, the immunogen is applied to a vertebrate (rabbit, sheep, etc.), usually with the aid of adjuvants which enhance immunogenicity, by a mechanism that is not fully understood. The production of polyclonal antibodies is not very reproducible. For this reason, antibody concentration (titer). 

Production of Polyclonal Anti-Plcloram Antibody. Picloram antisera was obtained from New Zealand White rabbits following the protocol described by Hall et al. (15). The rabbits were injected subcutaneously with an emulsion consisting of 0.5 to 1.0 mg immunogen dissolved in 0.5 mL of PBS and an equal volume of Freund s complete adjuvant. The injections were repeated 3, 6, and 10 days after the initial injection, substituting Freund s incomplete adjuvant for complete adjuvant. A booster injection was given one month after the initial injection and was repeated at monthly intervals thereafter. The rabbits were bled for antibody titer determinations 10 days after each boost. Antisera for picloram immunoassay development were prepared from a single bleed in each case. 

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