Spectroscopy surface plasmon resonance

Acronyms SPR spectroscopy (surface plasmon resonance spectroscopy) 

The surface resonance phenomenon in a thin metallic film is measured by attenuated total reflection as a function of either incident angle (using monochromatic light) or excitation wavelength (at a constant value of the incident angle). 

Ranges of operation from visible to infrared, typically visible. 

A SPR spectrometer has the following four principal components a source of radiation, an optical coupling element, a thin metallic film and a detector. Thin layers of SPR active metals are most often produced by vacuum evaporation or sputtering. 

Two arrangements of ATR optical prism couplers are used the Kretschmaim-type arrangement with a metallic film deposited directly onto the coupHng prism and the Otto-type arrangement with a metallic fihn separated from the coupling prism by air or other dielectric material of a thickness of about one wavelength of the radiation used in the experiments. The Kretchmann configuration is more convenient and is used in most applications. 

The method is sometimes called surface plasmon spectroscopy (SPS) the term resonance refers to the peculiarities of the reflectivity vs. angle of incidence plots obtained with the method (minimum of reflectivity). 

SPPs are sometimes also called PSPs (plasmon surface polaritons). 

The transversal electromagnetic modes are either photon-hke or similar to optical phonons, if their nature is a mix between these forms, the modes are sometimes called polaritons. 

Surface plasmon resonance has been used to study condensed films at metal/ solution interfaces [957, 958]. A typical result is displayed in Fig. 5.152. 

The SPR is found around ( o = 46°. From data taken at various electrode potentials, the number of organic species in the condensed thymine film could be calculated [957]. With a combination of SPR and EQCMB, the adsorption of a perfluo- 

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U. Kunz, A. Katerkamp, R. Renneberg, F. Spener, and K. Cammann, Sensing fatty acid binding protein with planar and fiber-optical surface plasmon resonance spectroscopy devices. Sens. Actuators B 32, 149-155 (1996). 

Shimizu KT, Pala RA, Fabbri JD, Brongersma ML, Melosh NA (2006) Probing molecular junctions using surface plasmon resonance spectroscopy. Nano Lett 6 2797-2803... 

Willets KA, Van Duyne RP (2007) Localized surface plasmon resonance spectroscopy and sensing. Annu Rev Phys Chem 58 267-297... 

The molecular interaction of cytochrome c and cardiolipin has been extensively studied. A mode of the interaction has been confirmed to be both electrostatic and hydrophobic, by using infrared spectroscopy (Choi and Swanson, 1995), fluorescence resonance energy transfer method (Rytdmaa and Kinnunen, 1994), protease digestion (de Jongh et al., 1995), cyclic voltammetry (Salamon and ToUin, 1997), deuterium and phosphorus NMR measurements (Spooner et al., 1993), and surface plasmon resonance spectroscopy (Salamon and Tollin, 1996). 

Optical sensors have the advantage of an easily measured signal that can be seen by the naked eye in some cases. Optical detection methods include fluorescence, surface plasmon resonance spectroscopy, Raman, IR, and chemiluminescence (Fabbrizzi and Poggi 1995 deSilva et al. 1997). However, the fabrication and development of optical MIP sensors requires that a colored, emissive, or fluorescent monomer... 

With surface plasmon resonance spectroscopy, kon and kQa rates of the ap tamer-target complex are determined. These in turn, allow calculation of the KD value [42]. For surface plasmon resonance spectroscopy, one of the two binding partners... 

Determining Binding Affinities Using Surface Plasmon Resonance Spectroscopy... 

Radioactivity, chemiluminescence or fluorescence have traditionally been used to monitor signals with protein arrays. The use of fluorescent dyes allows for increased sensitivity of detection, the ability to multiplex detection of several targets, and easy interfacing with detection instrumentation and automation. Surface plasmon resonance spectroscopy is another alternative, but successful development and commercialization has not yet been achieved. 

Reversible attachment of nanostructures at molecular printboards was exemplified by the adsorption and desorption of CD-functionalized nanoparticles onto and from stimuli-responsive pre-adsorbed ferrocenyl-dendrimers at a CD SAM (Fig. 13.7).65 Electrochemical oxidation of the ferrocenyl endgroups was employed to induce desorption of the nanostructure from the CD SAM. An in situ adsorption and desorption of ferrocenyl dendrimers and CD-functionalized Au nanoparticles (d 3 nm) onto and from the molecular printboard was observed by a combination of surface plasmon resonance spectroscopy (SPR) and electrochemistry. Similar behavior was observed when larger CD-functionalized silica nanoparticles (d 60 nm) were desorbed from the surface with the aid of ultrasonication. 

Haes AJ, van Duyne RP. A nanoscale optical biosensor Sensitivity and selectivity of an approach based on the localized surface plasmon resonance spectroscopy of triangular silver nanoparticles. J Am Chem Soc 2002 124 10596-604. 

Abstract Makrolon , a commercially available polycarbonate with a glassy ultramicroporous structure (mean pore-volume 0.1 nm3), was often employed as sensitive layer for optical sensors in recent years. Due to the definite pore volume-distribution, it can be used as a size-selective sensitive layer. The interaction behaviour of Makrolon of different layer-thicknesses under the influence of methanol, ethanol and 1-propanol is characterized by Spectral El-lipsometry (SE), Surface Plasmon Resonance Spectroscopy (SPR)... 

Spectral Ellipsometry Surface Plasmon Resonance Spectroscopy Ultramicroporous Polymer... 

A chemical sensor is a device that transforms chemical information into an analytically useful signal. Chemical sensors contain two basic functional units a receptor part and a transducer part. The receptor part is usually a sensitive layer, therefore a well founded knowledge about the mechanism of interaction of the analytes of interest and the selected sensitive layer has to be achieved. Various optical methods have been exploited in chemical sensors to transform the spectral information into useful signals which can be interpreted as chemical information about the analytes [1]. These are either reflectometric or refractometric methods. Optical sensors based on reflectometry are reflectometric interference spectroscopy (RIfS) [2] and ellipsometry [3,4], Evanescent field techniques, which are sensitive to changes in the refractive index, open a wide variety of optical detection principles [5] such as surface plasmon resonance spectroscopy (SPR) [6—8], Mach-Zehnder interferometer [9], Young interferometer [10], grating coupler [11] or resonant mirror [12] devices. All these optical... 

Keywords Hydrogel Photo cross-linking Poly(A-isopropy 1-acrylamide) Surface plasmon resonance spectroscopy... 

Tokareva I, Minko S, Fendler H, Hutter E (2004) Nanosensors based on responsive polymer brushes and gold nanoparticle enhanced transmission surface plasmon resonance spectroscopy. 1 Am Chem Soc 126 15950-15951... 

Salmon, Z., et al. (1997), Surface plasmon resonance spectroscopy as a tool for investigating the biochemical and biophysical properties of membrane protein system. II. Application to biological system, Biochim. Biophys. Acta, 1331,131-152. 

Su X, Wu Y-J, Knoll W (2005) Comparison of surface plasmon resonance spectroscopy and quartz crystal microbalance techniques for studying DNA assembly and hybridization. Biosens Bioelectron 21 719-726... 

Wang JL, Munir A, Zhou HS (2009) Au NPs-aptamer conjugates as a powerful competitive reagent for ultrasensitive detection of small molecules by surface plasmon resonance spectroscopy. Talanta 79 72-76... 

Su X, Neo SJ, Pek W, Thomsen IS (2008) A two-step antibody strategy for surface plasmon resonance spectroscopy detection of protein-DNA interactions in nuclear extracts. Anal Biochem 376 137-143... 

Sherry LJ, Chang SH, Schatz GC et al (2005) Localized surface plasmon resonance spectroscopy of single silver nanocubes. Nano Lett 5 2034—2038... 

R. S., Synthetic peptide vaccine development measurement of polyclonal antibody affinity and cross-reactivity using a new peptide capture and release system for surface plasmon resonance spectroscopy, J. Mol. Recog. 17, 540-557, 2004 Stills, H.F, Jr., Adjuvants and antibody production dispelling the myths associated with Freund s complete and other adjuvants, ILAR J. 46, 280-293, 2005 Miller, L.H., Saul, A., and Mahanty,... 

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