P388 mouse leukemia cells

In 1997, Chakrabarty et al. reported the isolation of 9-carbethoxy-3-methylcarba-zole (5) and 9-formyl-3-methylcarbazole (6) from the roots of M. koenigii (17). These metabolites are the first 9-formyl and 9-carbethoxy carbazole derivatives obtained from plant sources. 9-Formyl-3-methylcarbazole (6) showed weak cytotoxicity against both mouse melanoma B16 and adriamycin-resistant P388 mouse leukemia cell lines. The structural assignment of these two alkaloids was based on the IR- and H-NMR spectra which were lacking any signal of an NH group. Additional structural support for 9-carbethoxy-3-methylcarbazole (5) was provided by the similarity of the UV absorption spectrum with that of a synthetic sample, obtained by reaction of 3-methylcarbazole with ethyl chloroformate in the presence of base. Further structural support for 9-formyl-3-methylcarbazole (6) was derived from a comparison of the UV spectrum and the IR carbonyl absorption (1696 cm ) with those of an authentic sample of 9-formyl-3-methylcarbazole (1700 cm ), prepared by the treatment of 3-methylcarbazole (2) with 98% formic acid (17) (Scheme 2.3). 

Manzamines E and F, with a ketonic carbonyl group in the eight-membered ring portion of the molecule, were isolated from an Okinawan Xestospongia sp. and patented as antitumor agents against P388 mouse leukemia cells, in vitro [38]. 

Kohamaic acids A (9) and B (10) exhibited cytotoxicity against P388 mouse leukemia cells, with an IC50 of 17 and 2.8 Xg/mL, respectively. Kohamaic acids also completely inhibited the cleavage of fertilized sea urchin eggs at a concentration of 2 Xg/mL. 

Haterumadioxins A (20) and B (21) showed significant C5dotoxicity against P388 mouse leukemia cells, with IC50S of 11 and 5.5 ng/mL, respectively. Haterumadioxin A (20) was evaluated against a human... 

At nanomolar to micromolar concentrations, yessotoxin has been shown to be toxic to many mammalian cells in culture, including a BE(2)-M17 neuroblastoma cell line [37], a human neuroblastoma cell line [38], HeLa S3 cells [39], rat L6 and mouse BC3H1 skeletal muscle myoblast cell lines [40], P388 mouse leukemia cells [41], 3T3 mouse fibroblasts [42], rat hepatocytes [43], and isolated cerebellar neurons [44]. Yessotoxin did not cause significant mortality in MCE breast cancer cells at nanomolar concentrations, although growth was inhibited [45]. Toxicity to an insect cell line (IPLB-LdFB), derived from a lepidopteran larval fat body, has also been demonstrated [42]. 

The pinnatoxins show cytotoxicity against the P388 mouse leukemia cell line. The most toxic congener was pinnatoxin D, with an IC50 of approximately 3 jM [43],... 

A closely related approach was found in the synthesis of nakiterpiosin 43, an inhibitor of P388 mouse leukemia cells.In the synthesis, LiBr was used to substitute the aryl sulfonate moiety in 41 to yield the 6-Br key intermediate 42 (Scheme 42.13). 

P388 mouse leukemia at a dose of 100 iig/mouse/day without exhibiting any toxicity [266], 2,4-Disubstituted 1,3-selenazoles (164, 165), evaluated for their antitumor activity by determining their ability to inhibit proliferation of L1210 cells in vitro, exhibited appreciable activity, although the 1,3-selenazole (165) was less potent than the sulfur analog [267, 268],... 

Ecteinascidins (tunicate) Active in P388 mouse leukemia, L1210 cells 0.0001-0.08 Mg/ml 109... 

L-1210 and P388 - murine leukemia cells L5178 - mouse lymphoma cells KB - human nasopharyngeal cells A-549 - lung carcinoma cells... 

CCD-19Lu (human normal pulmonary cell) P388 (mouse leukemia)... 

Flavazole C-nucleosides revealed cytotoxic activity against the human nasopharynx KB epidermoid carcinoma cells (82MI4 84MI5) and mouse P388 leukemia cells (84MI5). 

Approximately lxlO5 P388 cells of murine leukemia cells were injected intraperitoneally into CDFj-SLC mice (10 weeks, female on day 0). Pironetin was administrated intraperitoeally daily for days. Each mouse was weighed on day 1 and 10, and mortality was monitored. 

Gymnodimine did not lyse mouse erythrocytes at a concentration of 10 jM, or cause toxic effects in mouse neuroblastoma NB41 cells or mouse leukemia P388 cells in vitro at the same concentration... 

The cytotoxicity of compounds 22-38 and 42-44 was evaluated against mouse lymphoc3dic leukemia (P388) cells. The toxicity of these compounds against fertilized sea urchin eggs was also evaluated. The results are shown in Table (2). 

A number of studies have analyzed the efficacy of berberine in vivo against transplanted hematologic tumor cell fines as well as during virus-induced leukemia. Specifically, it has been showed that berberine has antitumoral potential in vivo against transplanted mouse acute lymphocytic leukemia P388 [112] and acute myeloid leukemia WEHI-3 [151]. fii addition, this alkaloid was able to inhibit the progression of erythroleukemia induced by mouse rebovirus. Friend, in immunocompetent mice [152]. 

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