Leukemia mouse

Diastovaricias I (70) and II (71) are produced by Streptomyces diastochromogenes. Diastovaricias I and II are active against Friend mouse leukemia cells. Spectral data were used to determine the stmctures (226). 

Sleeves R, Lilly F (1977) Interactions between host and viral genomes in mouse leukemia. Ann Rev Genetics 11 277-296... 

Hepatite Virus NS3/4A having the pyrrolidine-5,5-trans-lactam skeleton [83], starting from (R)- and (S)-methionine, respectively. The key step is the addition of the proper silyl ketene acetal to an iminium ion, e.g., that generated by treatment of the intermediate 177 with boron trifluoride, which provided the adduct 178 with better diastereoselectivity than other Lewis acids. Inhibitors of hepatitis C virus NS3/4A were efficiently prepared by a similar route from (S)-methionine [83]. The addition of indole to a chiral (z-amino iminium ion was a completely diastereoselective step in a reported synthesis of tilivalline, a natural molecule which displays strong cytotoxicity towards mouse leukemia L 1210 [84]. 

Fluid-phase uptake of macromolecules by cells in general is a slow process, and most administered macromolecules are eliminated from the host before any significant cellular uptake takes place. If, however, the macromolecule contains a moiety that is compatible with a receptor on a specific cell surface, then the macromolecule is attracted to the cell surface and the uptake is enhanced. This maximizes the opportunity for specific-cell capture. This type of cell-specific targeting has been developed to hepatocytes, with galactosamine to T lymphocytes, with anti-T cell antibodies and to mouse leukemia cells, with fucosylamine and other biomolecules. 

Fig. 4. Flow cylometric patterns for L12I0 mouse leukemia cells in the absence (A) and presence (B) of vinblastine (9 hr exposure to 8 nM vinblastine). (Data courtesy of Dr. Linda Borman, Vermont Regional Cancer Center.)...

In 1997, Chakrabarty et al. reported the isolation of 9-carbethoxy-3-methylcarba-zole (5) and 9-formyl-3-methylcarbazole (6) from the roots of M. koenigii (17). These metabolites are the first 9-formyl and 9-carbethoxy carbazole derivatives obtained from plant sources. 9-Formyl-3-methylcarbazole (6) showed weak cytotoxicity against both mouse melanoma B16 and adriamycin-resistant P388 mouse leukemia cell lines. The structural assignment of these two alkaloids was based on the IR- and H-NMR spectra which were lacking any signal of an NH group. Additional structural support for 9-carbethoxy-3-methylcarbazole (5) was provided by the similarity of the UV absorption spectrum with that of a synthetic sample, obtained by reaction of 3-methylcarbazole with ethyl chloroformate in the presence of base. Further structural support for 9-formyl-3-methylcarbazole (6) was derived from a comparison of the UV spectrum and the IR carbonyl absorption (1696 cm ) with those of an authentic sample of 9-formyl-3-methylcarbazole (1700 cm ), prepared by the treatment of 3-methylcarbazole (2) with 98% formic acid (17) (Scheme 2.3). 

Conjugate showed concentration dependent cytotoxicity against P388 mouse leukemia... 

Powell described as early as 1970 the antitumor activity of the related harringtonins 2 5 with respect to the mouse leukemias P-388 and L-1210.6 Activity in terms of human cancers has also been established.7 and is currently the subject of clinical studies. 

Also 2 -halo-3 -hydroxy derivatives of various anthracyclinones show a high activity against P 388 mouse leukemia in certain in-vivo tests. A compound like 98 has been prepared in the 2-deoxy-2-iodo-a-L-ma no-series by Horton et al. [59, 60] starting from di-O-acetyl-L-rhamnal (28). Similarly, Thiem et al. have also successfully prepared the tetracenomycinone-C glycoside 99 [61]. From 4-0-acetyl-3-0-(p-methoxy)-benzyl-L-fucal (102) the glycoside derivatives 103 and 104 in the talo-series were obtained. 

In this communication we extend our earlier observations, which primarily dealt with the antiviral action of mouse fibroblast interferon, to its antigrowth activity and to antiviral and antigrowth activities of mouse T-cell interferon. We will show that inhibition by common gangiiosides Is restricted to both activities of fibroblast interferon alone. T-cell interferon, although its biological activities are analogous to those of fibroblast Interferon, neither binds to nor Is inhibited by these glycol ipids. Furthermore we demonstrate that mouse leukemia L—1210 cells that were selected for resistance to fibroblast interferon (6), respond equally well to T-cell Interferon as the parent cells which are responsive to both Interferons. 

In cultured mouse leukemia cells L1210, inhibited DNA, RNA, and protein synthesis 504,505... 

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