P388 mouse leukemia

In 1997, Chakrabarty et al. reported the isolation of 9-carbethoxy-3-methylcarba-zole (5) and 9-formyl-3-methylcarbazole (6) from the roots of M. koenigii (17). These metabolites are the first 9-formyl and 9-carbethoxy carbazole derivatives obtained from plant sources. 9-Formyl-3-methylcarbazole (6) showed weak cytotoxicity against both mouse melanoma B16 and adriamycin-resistant P388 mouse leukemia cell lines. The structural assignment of these two alkaloids was based on the IR- and H-NMR spectra which were lacking any signal of an NH group. Additional structural support for 9-carbethoxy-3-methylcarbazole (5) was provided by the similarity of the UV absorption spectrum with that of a synthetic sample, obtained by reaction of 3-methylcarbazole with ethyl chloroformate in the presence of base. Further structural support for 9-formyl-3-methylcarbazole (6) was derived from a comparison of the UV spectrum and the IR carbonyl absorption (1696 cm ) with those of an authentic sample of 9-formyl-3-methylcarbazole (1700 cm ), prepared by the treatment of 3-methylcarbazole (2) with 98% formic acid (17) (Scheme 2.3). 

Conjugate showed concentration dependent cytotoxicity against P388 mouse leukemia... 

P388 mouse leukemia at a dose of 100 iig/mouse/day without exhibiting any toxicity [266], 2,4-Disubstituted 1,3-selenazoles (164, 165), evaluated for their antitumor activity by determining their ability to inhibit proliferation of L1210 cells in vitro, exhibited appreciable activity, although the 1,3-selenazole (165) was less potent than the sulfur analog [267, 268],... 

Manzamines E and F, with a ketonic carbonyl group in the eight-membered ring portion of the molecule, were isolated from an Okinawan Xestospongia sp. and patented as antitumor agents against P388 mouse leukemia cells, in vitro [38]. 

Echinatine-A -oxide Active against P388 mouse leukemia — 311... 

Ecteinascidins (tunicate) Active in P388 mouse leukemia, L1210 cells 0.0001-0.08 Mg/ml 109... 

Kohamaic acids A (9) and B (10) exhibited cytotoxicity against P388 mouse leukemia cells, with an IC50 of 17 and 2.8 Xg/mL, respectively. Kohamaic acids also completely inhibited the cleavage of fertilized sea urchin eggs at a concentration of 2 Xg/mL. 

Haterumadioxins A (20) and B (21) showed significant C5dotoxicity against P388 mouse leukemia cells, with IC50S of 11 and 5.5 ng/mL, respectively. Haterumadioxin A (20) was evaluated against a human... 

At nanomolar to micromolar concentrations, yessotoxin has been shown to be toxic to many mammalian cells in culture, including a BE(2)-M17 neuroblastoma cell line [37], a human neuroblastoma cell line [38], HeLa S3 cells [39], rat L6 and mouse BC3H1 skeletal muscle myoblast cell lines [40], P388 mouse leukemia cells [41], 3T3 mouse fibroblasts [42], rat hepatocytes [43], and isolated cerebellar neurons [44]. Yessotoxin did not cause significant mortality in MCE breast cancer cells at nanomolar concentrations, although growth was inhibited [45]. Toxicity to an insect cell line (IPLB-LdFB), derived from a lepidopteran larval fat body, has also been demonstrated [42]. 

The pinnatoxins show cytotoxicity against the P388 mouse leukemia cell line. The most toxic congener was pinnatoxin D, with an IC50 of approximately 3 jM [43],... 

Prodrugs of DME>C were also synthesized and evaluated to be active against P388 mouse leukemia in vivo. Antiviral activity of DMDC and its derivatives were reported to be effective against HSV-1, VZV, and HCMV.4l.43... 

A closely related approach was found in the synthesis of nakiterpiosin 43, an inhibitor of P388 mouse leukemia cells.In the synthesis, LiBr was used to substitute the aryl sulfonate moiety in 41 to yield the 6-Br key intermediate 42 (Scheme 42.13). 

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