P388 leukemia cells

The toxic mushroom Russula subnigricans produces the novel polychlorinated phenyl ethers, russuphelins A-F (1928-1933), some of which (B, C, D) exhibit cytotoxicity against P388 leukemia cells (1672, 1800). Further study of this mushroom uncovered the presence of the optically active russuphelol (1934) (1801). The terrestrial cyanobacterium Fischerella ambigua has afforded ambigol C (1935) (1654), which is an isomer of the previously described ambigols A and B (1). 

Isovoacangine Inhibition of P388 leukemia cells 18 jLtg/ml 281... 

The fluorescent complex [Ru(II)(3,4,7,8-tetramethylphen)3]Cl2 is readily taken up by P388 leukemia cells in culture and is visible on the cell surface, in the cytoplasm, and in the nucleus, but it does not exhibit antitumor activity in vivo (11). The platinum complexes [Pt(en)(oxalate)], [Pt(NH3)2(H20)2l (as the dinitrato complex), and [Pt(en)3] + also cause convulsions in animals (12) the latter two complexes are positively charged and relatively inert and the former is neutral and presumably undergoes an activation step before it binds to the neuromuscular junction. Curiously, the related malonato complex is not a neurotoxin. Care is taken in the clinic to administer cisplatin in saline solutions to avoid hydrolysis and minimize the production of neurotoxic aqua or hydroxobridged Pt(II) species. 

Cytotoxic activities of compound XVIII against murine P388 leukemia cells ... 

Flavazole C-nucleosides revealed cytotoxic activity against the human nasopharynx KB epidermoid carcinoma cells (82MI4 84MI5) and mouse P388 leukemia cells (84MI5). 

Marine sponges are rich sources of bioactive cerebrosides. Hirsch s group5 isolated halicerebroside A 2, which exhibited mild antitumor activity against P388 leukemia cells, from the Haliclona sp. sponge. 

All compounds seem to exhibit potent biological activity although a discrepancy exists regarding the cytotoxicity of chondrillin [1] in the P388 leukemia cell assay, where Sakemi et al. (4) found an IC50 of 5 pg/ml whereas De Guzman et al. 

Plasmodium Ealciparum and Murine P388 Leukemia Cell Assay 555... 

Haliclamines A (43) and B (44). Isolated from Haliclona sp., the structures of these reduced analogs were deduced by homonuclear and heteronuclear COSY and HMBC spectra. 43 and 44 showed a 1,3-disubstituted-3,4-dehydropiperidine moiety that is also present in saraines and isosaraines. Both compoimds not only inhibit cell division of fertilized sea urchin eggs, but also growth of L1210 (IC50 1-5 and 0.9 jug/ml, respectively) and P388 leukemia cell lines (0.75 and 0.39) [40]. 

Bailly and coworkers were interested in synthesizing structures containing a carbazole nucleus with a fused imide ring and examining their effects on DNA, human topoisomerases, and P388 leukemia cells [104]. Thus, the Diels-Alder reaction between the 3-vinylindole species 292 and DMAD (221) was used as the key synthetic step to provide the cycloaddition product 293. This cycloadduct was in turn oxidized to the carbazole moiety 294, bearing a diester functionality that could then be cyclized with an appropriate amine 295 to produce the A-substituted imide ring in the final product 296 (Scheme 62). 

The bioactive pyirolo[4,3,2-de]quinoline alkaloids B. are isolated from the Caribbean sponge Batzella sp., living at a depth of about 120 m. B. AC,2H,, C1N2C>2S, Mr 282.74, black prisms, mp. 205 °C isobatzellineA C12H12CIN3OS, Mr 281.77, brown solid, active against P388 leukemia cells and Candida albicans. They may be biosynthetic precursors of the discorhabdins. 

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