Ovarian cancer cells

Proteomic Mapping and Clustering of Multiple Samples—Application to Ovarian Cancer Cell Lines... 

He et al. (2002) used an off-line HPLC/CE method to map cancer cell extracts. Frozen ovarian cancer cells (containing 107 cells) were reconstituted in 300 pL of deionized water and placed in an ultrasonic bath to lyse the cells. Then the suspension was centrifuged and the solubilized proteins were collected for HPLC fractionation. The HPLC separation was carried out on an instrument equipped with a RP C-4 column, 250 mm x 4.6 mm, packed with 5-pm spherical silica particles. Extracted proteins were dissolved in 300 pL of DI water, and lOOpL was injected onto the column at a flow rate of 1 mL/min. Buffer A was 0.1% TEA in water and buffer B was 0.1% TFA in acetonitrile. A two-step gradient, 15-30% B in 15 min followed by 30-70% B in 105 min, was used. The column effluent was sampled every minute into a 96-well microtiter plate with the aid of an automatic fraction collector. After collection, the fractions were dried at room temperature under vacuum. The sample in each well was reconstituted before the CE analysis with 10 pL deionized water. The... 

Pirker, R., Fitzgerald, D.J.P., Hamilton, T., Ozols, R.F., Laird, W., Frankel, A.E., Willingham, M.C., and Pastan, I. (1986) Characterization of immunotoxins active against ovarian cancer cell lines. J. Clin. Invest. 76, 1261. 

SH-SY5Y neuroblastoma, cisplatin-sensitive A2780 and cispla-tin-resistant A2780cis human ovarian cancer cells was observed, but upon irradiation 7 strongly reduced the viability of the cancer cells (Fig. 8). In the A2780 cell line, the complex was 80x more toxic than cisplatin under identical conditions, and ca. 15 x more effective against the cisplatin-resistant A2780cis cell line (33). The trans diazido-Pt(IV) complex therefore has remarkable cytotoxic properties. 

Fig. 4.9 Comparison of PDT-induced killing in human ovarian cancer cells by BF4 or Photofrin both incubated for 24 h at 2(uM concentration and illuminated with red (630 nm) or white light...

Kikuchi, Y., Sasa, H., Kita, T., Hirata, J., Tode, T., and Nagata, I. (1991). Inhibition of human ovarian cancer cell proliferation in vitro by ginsenoside Rh2 and adjuvant effects of cisplatin in vivo. Anticancer Drugs 2, 6S-67. 

A series e t-kaurane diterpenoids isolated from Sideritis species were tested against A2780 ovarian cancer cell lines, and 7-e/>/candicandiol (Fig. 6.6) showed the highest cytotoxic potential with ICj , = 9.0 pg/mL, and sidol followed it (ICj , = 15.6 pg/mL). 

It was carried out according to Schwikkard et al. [9] and Actinomycin D was used as a positive control [ 16]. Cytotoxicity was determined against A2780 human ovarian cancer cells, using a microtiter plate assay. The plates were seeded with cells and... 

Fig. 2. Fluorescence image of surface localization in cultured cells. OVCAR-3 human ovarian cancer cells were incubated with monoclonal antibody Kl, followed by antimouse IgG conjugated to rhodamine as described in the protocol (3). The intense diffuse speckled surface fluorescence image is shown on multiple cells in this area of the culture, with reinforced bright images at the vertical cell margins generating a cobblestone appearance (bar = 4 jm).

Cartee L, Kucera GL (1998) Gemcitabine Induces programmed cell death and activates protein kinase C in BG-1 human ovarian cancer cells. Cancer Chemother Pharmacol 41 403-412... 

Ma J, Maliepaard M, Kolker HJ, Verweij J, Schellens JHM (1998a) Abrogated energy-dependent uptake of cisplatin in a cisplatin-resistant subline of the human ovarian cancer cell line IGROV-1. Cancer Chemother Pharmacol 41 186-192... 

Ma I, Maliepard M, Nooter K, Loos WJ, Kolker HJ, Verweij J, Stoter G, Schellens JHM (1998b) Reduced cellular accumulation of topotecan a novel medianism of resistance in a human ovarian cancer cell line. Br ) Cancer 77 1645-1652 MacFarlane DE, O Donnell PS (1993) Phorbol ester induces apoptosis in HL-60 promyelocytic leukemia cells but not in HL-60 PET mutant. Leukemia 7 1846-1851... 

Arora S, Bisanz KM, Peralta LA et al (2010) RNAi screening of the kinome identifies modulators of cisplatin response in ovarian cancer cells. Gynecol Oncol 118 220-227... 

Barres V, OueUet V, Lafontaine J et al (2010) An essential role for Ran GTPase in epithelial ovarian cancer cell survival. Mol Cancer 9 272... 

Vikhanskaya F, Vignati S, Beccaglia P, et al. Inactivation of p53 in a human ovarian cancer cell line increases the sensitivity to paclitaxel by inducing G2/M arrest and apoptosis. Exp Cell Res 1998 241(1) 96-101. 

Giannakakou P, Sackett DL, Kang YK, et al. Paclitaxel-resistant human ovarian cancer cells have mutant beta-tubulins that exhibit impaired paclitaxel-driven polymerization. J Biol Chem 1997 272(27) 17,118-17,125. 

B5. Bertoni, R, Concord, A., Carobbio, S., ReaUni, C., Codegoni, A. M., Zucca, E., and CavalU, F., Analysis of Fas/CD95 gene somatic mutations in ovarian cancer cell lines Petter]. Int. J. Cancer 86, 450 (2000). 

Shaw, T.J., E.J. Keszthelyi, A.M. Tonary, M. Cada, and B.C. Vanderhyden, Cyclic AMP in ovarian cancer cells both inhibits proliferation and increases c-KIT expression. Exp Cell Res, 2002. 273(1) 95-106. 

It has been demonstrated that the human myeloid zinc finger (MZF1) (92) functions as a transcription repressor regulating ERCCl transcription in response to cisplatin-induced DNA damage. It was found that MZFl mRNA is constitutively expressed in human ovarian cancer cells. Quantitative PCR in the same cells showed that MZFl mRNA was decreased upon cisplatin exposure (93). Therefore, decreased expression of MZFl is a mechanism to be further investigated. 

Wang X, Jin DY, Ng RW et al. Significance of MAD2 expression to mitotic checkpoint control in ovarian cancer cells. Cancer Res 2002 62 1662-1668. 

Li Q, Gardner K, Zhang L et al. Cisplatin induction of ERCC-1 mRNA expression in A2780/CP70 human ovarian cancer cells. J Biol Chem 1998 273 23419-23425. 

---