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RNAi screens

Seyhan AA, Rya TE (2010) RNAi screening for the discovery of novel modulators of human disease. Curr Pharm Biotechnol 11 735-756... [Pg.87]

High-Throughput RNAi Screening for the identification of Novei Targets... [Pg.89]

HT-RNAi screens of mammalian cells primarily use either short interfering RNA (siRNA) or small-hairpin RNA (shRNA) libraries. Although there are similarities between the two platforms, screening using siRNA libraries has the advantages of having minimal... [Pg.89]

Fig. 1. Fligti-ttiroughput RNAi screening using siRNA libraries. Assay plates containing library siRNA are treated with (1) diluted transfection reagent to complex the siRNA and lipid. (2) Cells are added to initiate the transfections. Assay plates are incubated for 72-96 h then (3) readout reagent is added. Assay plates are read and the data is analyzed (4-5). Fig. 1. Fligti-ttiroughput RNAi screening using siRNA libraries. Assay plates containing library siRNA are treated with (1) diluted transfection reagent to complex the siRNA and lipid. (2) Cells are added to initiate the transfections. Assay plates are incubated for 72-96 h then (3) readout reagent is added. Assay plates are read and the data is analyzed (4-5).
Henderson MC, Gonzales IM, Arora S et al (2011) High-throughput RNAi screening identifies a role for TNKl in growth and survival of pancreatic cancer cells. Mol Cancer Res 9 724-732... [Pg.95]

Tyner JW, Walters DK, Willis SG et al (2008) RNAi screening of the tyrosine kinome identifies therapeutic targets in acute myeloid leukemia. Blood 111 2238-2245... [Pg.95]

Cole KA, Huggins J, Laquaglia M et al (2011) RNAi screen of the protein kinome identifies checkpoint kinase 1 (CHKl) as a therapeutic target in neuroblastoma. Proc Natl Acad Sci USA 108 3336-3341... [Pg.95]

MacKeigan JP, Murphy LO, Blenis J (2005) Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance. Nat Cell Biol 7 591-600... [Pg.95]

Azorsa DO, Gonzales IM, Basu CD et al (2009) Synthetic lethal RNAi screening identifies sensitizing targets for gemcitahine therapy in pancreatic cancer. J Transl Med 7 43... [Pg.95]

Berns K, Hijmans EM, Mullenders J et al (2004) A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428 431-437... [Pg.95]

Arora S, Bisanz KM, Peralta LA et al (2010) RNAi screening of the kinome identifies modulators of cisplatin response in ovarian cancer cells. Gynecol Oncol 118 220-227... [Pg.95]

Arora S, Beaudry C, Bisanz KM et al (2010) A high-content RNAi-screening assay to identify modulators of cholesterol accumulation in Niemann-Pick type C cells. Assay Drug Dev Technol 8 295-320... [Pg.95]

Information of our chemical and siRNA libraries is stored in a PostgreSQL database. We annotate our validated hits using a DatabaseReader and a Joiner node to obtain either chemical structures for chemical screens or GenelD for RNAi screens. KNIME has many tools for cheminformatics, can visualize the molecule structures, and has tools to retrieve data from external public databases via Web queries to further annotate hits, allowing clustering either by chemical substructures or GO terms. [Pg.120]

Conrad C, GerHch DW (2010) Automated microscopy for high-content RNAi screening. JCB 188 453 61... [Pg.122]

Luo J, Emanuele MJ, Li D et al (2009) A genome-wide RNAi screen identifies multiple synthetic lethal interactions with the Ras oncogene. Cell 137 835-848... [Pg.136]

Falschlehner C, Steinbrink S, Erdmann G et al (2010) High-throughput RNAi screening to dissect cellular pathways a how-to guide. Biotechnol J 5 368-376... [Pg.322]

Seyhan AA, Varadarajan U, Choe S et al (2011) A genome-wide RNAi screen identifies novel targets of neratinib sensitivity leading to nera-tinib and paclitaxel combination drug treatments. Mol BioSyst 7 1974-1989... [Pg.322]

Zhu YX, Tiedemann R, Shi CX et al (2011) RNAi screen of the druggable genome identifies modulators of proteasome inhibitor sensitivity in myeloma including CDK5. Blood 117 3847-3857... [Pg.322]

After the selection of the specific techniques to be employed, one must determine how to quantify the genes associated with the phenotype of interest. The design of an assay that acts as a reporter or a molecular sensor can prove to be the most challenging step in designing a successful RNAi screen. The assay must be both highly reproducible and sensitive, without noise, and exhibiting minimal standard error. Traditional assay development techniques must be taken into account for all RNAi screens as they are for conventional compound-based screens. [Pg.163]

Li, S. et al. 2006. RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-xB signaling. Mol. Cell 24, 497-509. [Pg.167]

Ovcharenko, D. et al. 2005. High-throughput RNAi screening in vitro from cell lines to primary cells. RNA 11,985-993. [Pg.167]

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See also in sourсe #XX -- [ Pg.89 , Pg.94 , Pg.120 , Pg.230 ]



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RNAi screening

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