Osteoblast-like cell adhesion

In microsphere sintering, pre-synthesized polymer microspheres or polymer/ceramic/bioactive addiction composites are sintered to produce a 3-D porous scaffold (56). Bioactive scaffolds can be fabricated through this technique, and they are demonstrated to be supportive to human osteoblast-like cells adhesion, growth, and mineralization (57). Scaffolds fabricated through this technique can have graded porosity structures. Mechanical properties close to cancellous bone also become possible when the microspheres are sintered into... 

PLGA/HAp composites were also produced by colloidal non-aqueous chemical precipitation at room temperature by Petricca and coworkers [158]. The shape of the scaffolds with homogeneous distribution of HAp particles in PLGA matrix was easily engineered, and the mechanical properties of the PLGA/HAp composites indicated that the materials are suitable as bone substitution scaffolds. The decreased tensile strength was attributed to the presence of micropores within the scaffold, which are created upon removal of the ammonium nitrate, a by-product of the synthesis. The novelty of the process is the ability to incorporate up to 30 wt% HAp within the polymer matrix, thereby maximizing the material osteoconductivity, as evidenced by osteoblast-like cell adhesion experiments. 

Tryoen-Toth P, Vautier D, Haikel Y et al (2002) Viability, adhesion, and bone phenotype of osteoblast-like cells on polyelectrolyte multilayer films. J Biomed Mater Res 60 657-667... 

Huang H-H, Pan S-J, Lai Y-L, Lee T-H, Chen C-C, Lu F-H. Osteoblast-like cell initial adhesion onto a network-structured titanium oxide layer. Scr Mater... 

Wan Y, Wang Y, Liu Z, Qu X, Han B, Bei J, et al. Adhesion and proliferation of OCT-1 osteoblast-like cells on micro-and nano-scale topography structured poly (L-lactide). Biomaterials 2005 26 4453-9. 

Lopez-Perez, P.M., Marques, A.P., da SUva, R.M.P., Pashkuleva, I. and Reis, R.L. 2007. Effect of chitosan membrane surface modification via plasma induced polymerization on the adhesion of osteoblast-like cells. 

Rezania, A., and Healy, K. E. (1999), Biomimetic peptide surfaces that regulate adhesion, spreading, cytoskeletal organization, and mineralization of the matrix dqxrsited by osteoblast-like cells, Biotechnol. Prog. 15(1) 19-32. 

Bernstein A, Techlenburg K, Siidkamp P et al (2012) Adhesion and proliferation of human osteoblast-like cells on different biodegradable implant materials used for graft fixation in ACL-reconstruction. Arch Orthop Trauma Surg 132 1637-1645... 

For bone tissue regeneration, three-dimensional porous biomimetic hydroxy-apatite/collagen composites crosslinked by mTGase were developed [23]. Again, the enzyme was used with the main purpose to increase the mechanical resistance of the organic matrix. The obtained composites supported adhesion, proliferation, viability and differentiation of MG63 osteoblast-like cells and human umbilical vein endothelial cells (Fig. 2). 

Combinatorial libraries were employed to optimize microphase domain size and shape of polyethylene glycol (PEG) - poly(caprolactone) (PCL) polyurethanes, and their blends with PCL. Polyurethanes are used as biomaterials in a variety of applications, and the ability to tune microdomain size to enhance cell attachment is a desirable objective. These materials incorporate biocompatible PCL microdomains, to which cells can adhere, into a relatively non-adhesive PEG-PCL matrix. A region of chain extender composition and curing temperature, with controllable spherical-, wormlike-, and ribbon-shaped microdomains, was located through a series of increasingly focused libraries. Osteoblast-like cells (MC3T3-E1) cultured directly on selected libraries showed attachment density correlated with the distance to and size of nearby PCL microdomains. 

A.J. Garcia, P. Ducheyne, D. Boettiger, Effect of surface reaction stage on fibronec-tin-mediated adhesion of osteoblast-like cells to bioactive glass, J. Biomed. Mater. Res. 40 (1998) 48-56. 

Kornu R, Maloney WJ, Kelly MA and Smith RL (1996) Osteoblast adhesion to orthopaedic implant alloys. Effects of cell adhesion molecules and dia-mond-like carbon coating. J Orthop Res 14(6) 871-877. 

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