Of gangliosides from

Comparison of glycosphingolipids from human and chicken skeletal muscle. The elution of gangliosides from DEAE-Sephadex A 50 column with these 0.01, 0.02 and 0.2 M sodium acetate concentrations separated the gangliosides into mono-, di- and poly-sialo- fractions. The gangliosides of human muscle are shown in Fig. 1A. The monosialogangliosides GM3, GM2 and GM1 were eluted with 0.01 M sodium acetate in methanol (lane 2), GD3 and GDla with the 0.02 M solvent (lane 4) and others with 0.2 M acetate... 

Carter, T. P. and Kanfer, J. N. (1973) Methodology for separation of gangliosides from potential water-soluble precursors, Lipids 8, 537-548. 

A. Zamfir, Z. Vukelif, L. Bindila, J. Peter-KataUm9, R. Almeida, A. Staling, and M. AUen, Fully-automated chip-based nanoelectiospray tandem mass spectrometry of gangliosides from human cerebellum, J. Am. Soc. Mass Spectrom 15, 1649-1657 (2004). 

Rementzis, J. Antonopoulou, S. Demopoulos, C.A. (1997). Identification and study of gangliosides from Scomber scombrus muscle. Journal of Agricultural and Food Chemistry, Vol.45, No.3, (March 1997), p>p. 611-615, ISSN 0021-8561. 

The isolation of gangliosides from brain has been reviewed by Kanfer (1969) and by Bergelson (1980). Other specific references are Suzuki (1965), Sven-nerholm et al. (1972) and Laine et al (1974), and general comments on ganglioside separations will be found in Kates (1972) and Christie (1982). 

On-hne high-performance TLC (HPTLC) MALDI-FT-MS at elevated pressures was described in the direct analysis of gangliosides from TLC plates using soHd matrices [176]. Gangliosides are glycosphingolipids that contain one or more siahc... 

Several authors have reported the separation of gangliosides from animal organs, using TLC [32, 107, 146, 218]. TLC has however been devoted in particular to isolation of human gangliosides and to the analysis of their degradation products. This is discussed in the following section. 

Table 4. Labeling in vitro of gangliosides from subfractions of crude mitochondrial fraction obtained at 17,000g for 60 min and disrupted hypoosmotically...

Table 5. Ratios of the specific radioactivities of gangliosides from subfraction G and subfraction E or synaptosomal at short and long times after injection of N- H-acetylmannosamine to rats...

Table 6. Effect of colchicine on the labeling "in vivo and "in vitro of gangliosides from the visual system of the chicken...

Advances in glycolipid chemistry include the isolation of new types of gangliosides from human erythrocyte membranes and the elucidation of information on their blood-group activities. The importance of polyisoprenol-sugar derivatives continues to attract much effort and the enzyme work on rat liver and yeast membranes are worth consideration. Another advance is the discovery that neuraminidases which are also able to hydrolyse the appropriate bond in the ganglioside Gmi do exist. This bond was thought to be resistant to enzymic attack due to steric hindrance. 

Popa, I., Vlad, C., Bodennec, J. and Portoukalian, J. (2002) Recovery of gangliosides from aqueous solutions on styrene-divinylbenzene copolymer colmnns. J. Lipid Res. 43, 1335-1340. 

Three different types of gangliosides from bovine brain purchased from Sigma Chemical Company were used, which were monosialoganglioside (II NeuAea-... 

Puro and Keranen (1969) demonstrated that 10-33% of the constituent fatty acids of bovine kidney gangliosides are hydroxy fatty acids. The major fatty acid components of gangliosides from bovine lens are palmitic, oleic, and nervonic acid (Feldman et ah, 1966). 

Fig. 5. Relative distribution of gangliosides from the central nervous system.

Hanisch, F.-G., Witter, B., Crombach, G. A., Schanzer, W., and Uhlenbmck, G., 1992, N-Gly-colylneuraminic acid is a chemical marker of gangliosides from human breast carcinoma, in Tumor-Associated Antigens, Oncogenes, Receptors, Cytokines in Tumor Diagnosis and Therapy... 

Ishizuka, I., Kloppenburg, M., and Wiegandt, H., 1970, Characterization of gangliosides from fish brain, Biochim. Biophys. Acta 210 299. 

Price, H. C., Kundu, S., and Ledeen, R., 1975, Structures of gangliosides from bovine adrenal mudulla. Biochemistry 14 1512. 

Stone, A. L., and Kolodny, E. H., 1971, Circular dichroism of gangliosides from normal and Tay-Sachs tissues, Chem. Phys. Lipids 6 274. 

Schengrund, C. L., and Garrigan, O. W., 1%9, A comparative study of gangliosides from brains of various species. Lipids 4 488. 

Smith, W. G., 1966, Release of ganglioside from guinea-pig lung tissue during anaphylaxis. Nature (Lond.) 209 1251. 

FIGURE 6. Thin-layer chromatogram of gangliosides from Balb 3T3 and KBalb cells. Lane 1— gangliosides from normal contact-inhibited Balb 3T3 cells, Lanes 2 and 4— authentic ganglioside standards (same as Figure 5). Lanes 3 and 5— gangliosides from two batches of KBalb, a nonproductively Kirsten sarcoma-virus-transformed subclone of Balb 3T3. Reproduced with permission from Fishman et aL, 1973. 

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