Blood group B-active

Fucolipids are present in both compartments. However, the blood group B active compound of non-epithelial cells (absent in epithelial cells) is based on GalNAc while the H and A active substances specific for epithelial cells have GlcNAc in their core saccharide. In fact, GlcNAc seems to be absent from all non-epithelial glycolipids. The minor fucolipid based on tetrahexosyl-ceramide (as indicated in fraction B of Fig. 5) was obtained from pooled tissue. This glycolipid has been shown to be located in the epithelial cells. 

The blood-group B-active glycosphingolipids B-I (5) and B-II (6) were degraded by a-D-galactosidase and by a-L-fucosidase the assigned structures were based... 

Glycosphingolipids derived from series III, which show blood group activity in the A, B, O (H), Le , Le - system, were observed by Hakomori and Strycharz (1968) and Hakomori and Andrews (1970). A blood group B active oligohexosyl ceramide was isolated from rabbit erythrocytes and the following structure proposed (Eto et al., 1968) ... 

Bouchours, J., Bouchours, D., and Hansson, G. C., 1987, Developmental changes of gangliosides of the rat stomach. Appearance of a blood group B-active ganglioside, J. Biol. Chem. 262 16370-16375. 

Although no complete investigations of the structure of the blood group substances have yet been reported, it is evident from the information available that all the material so far studied is predominantly carbohydrate in nature, and, as can be seen from Table III, preparations from widely different sources have many properties in common, for example, elementary analysis, specific rotation and the like. It is of interest to note that almost all the substances obtained from nonhuman sources display blood group A or blood group O activity, while human material is the most convenient source of the blood group B substance. The majority of the work carried out so far has dealt with blood group A substances. 

Figure 9. Open tubular gas chromatogram of partially methylated alditol acetates obtained from blood-group A active tetraglycosylceramide (A) and hexaglycosyl-ceramide (B), respectively. Stationary phase was OV-1, and carrier gas was Ns. Column temperature was kept at 175°C for 14 min, then raised l°C/min. The designation above the peaks indicate actual binding positions.

Kallin, E, Lonn, H, Norberg, T, Glycosidations with thioglycosides activated by suUuryl chloride trifluoromethanesulfonic acid — synthesis of a human-blood group-B trisaccharide glycoside, Glycoconjugate J., 5, 3-8, 1988. 

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