Nitrogen analysis Kjeldahl method

Example Figure 3.1, represents the absolute errors in nitrogen analysis by means of micro Kjeldahl s Method. Here, each vertical line labelled (xj - xt) designates the absolute deviation of the mean of the set from the true value. In Figure 3.1, A represents (xj - xf) the absolute error obtained by analyst-1 for the assay of benzyl-iso-thioureahydrochloride, whereas B represents (x2 - x() the absolute error obtained by analyst-2 for the assay of the same compound. 

Total nitrogen determinations in barley and malt gave slightly higher results by the Dumas than by the Kjeldahl method. The Dumas method was adopted as the reference method by the Analysis Committee of the Institute of Brewing27. Comparison of the results obtained by the Kjeldahl method with those of automatic N analyzers is mentioned in Section III.A.2 above. 

The determination of nitrogen in petroleum products is performed regularly by the Kjeldahl method (ASTM D3228), the Dumas method, and the microcoulomet-ric (ASTM D3431) method. The chemiluminescence method is the most recent technique applied to nibogen analysis for petroleum and is used to determine the amount of chemically bound nitrogen in liquid samples. 

Kjeldahl method analychem Quantitative analysis of organic compounds to determine nitrogen content by interaction with concentrated sulfuric acid ammonia Is distilled from the Nff4S04 formed. kel,dal, meth-ad )... 

The purity of the recrystallized DL-aspartic acid was established by nitrogen analysis by the Kjeldahl and Van Slyke methods. The decomposition point of this product is 325-348°. 

Both the light scattering and infrared techniques are widely used, and there are many reports of the correlation between one of these methods and a reference method, usually Babcock or Gerber in the case of fat analysis and total nitrogen by Kjeldahl for proteins. One source of disparity between the various correlations has been the choice of a reference method. The realization that different methods for fat analysis measure different chemicals and thus can be expected to give different results has been slow in reaching the industry. 

Dry the feces in a vacuum oven overnight, or lyophilize and grind, for nitrogen analysis using a Kjeldahl method (unitbi.2). 

As with the products of polycondensation, the products of mechanochemical complexation were characterized by analysis of chemically linked nitrogen by ligand synthesis (Kjeldahl method), elementary analysis, and IR spectroscopy. The nitrogen variance for different working conditions is discussed elsewhere in this paper results of the elementary analysis are in Table III. IR spectra confirm that the ligand has the same structure as the polycondensation products, obeying the rule that for short durations, the band for ester carbonyl remains unaltered. For longer times, this band disappears. 

The standard procedure of nitrogen determination by many laboratories is the Kjeldahl method (ASTM D-3179 ISO 333), although there are the standard methods that involve the Dumas technique (DIN 51722) and the gasification procedure (DIN 15722). Neutron activation analysis has also been proposed for the determination of nitrogen in coal, coal ash, and related products (Volborth, 1979a). 

L9. Levin, B., and Oberholzer, V. G., Paper electrophoresis of serum proteins with micro Kjeldahl nitrogen analysis of the protein fractions A comparison with free electrophoresis and salt fractionation methods. Am. J. Clin. Pathol. 23, 205 (1953). 

Analysis. The heating value, ash content, and sulfur distribution of the raw and treated coals were determined according to ASTM procedures (7). Iron in the extracts of the raw and treated coals was determined by titration with a cerium (IV) solution. Iron in the residues from the acid extractions of the raw and treated coals was determined spectrophotometrically using ferrozine (20). The liquid extracts were analyzed for total sulfur (as sulfate) by ion chromatography after separation of the sulfate from nitrate on an alumina column (21). Nitrogen was determined in the raw and treated coal and in their nitric acid-extracted residues by a modified Kjeldahl method. 

Nitrogen by the Kjeldahl Method.— The absolute method, while the more accurate and often used when the results of analysis are for the purpose of determining the empirical formula of a compound, is sometimes replaced by the Kjeldahl or wet combustion process. In outline the method is as follows The organic compound is decomposed and oxidized by heating for some time in about 30 cc. of pure con-... 

Paper is an important filtering medium. Ashless paper is manufactured from cellulose fibers that have been treated with hydrochloric and hydrofluoric acids to remove metallic impurities and silica ammonia is then used to neutralize the acids. The residual ammonium salts in many filter papers may be sufficient to affect the analysis for nitrogen by the Kjeldahl method (see Section 37C-11). 

Elemental analysis is probably the oldest method that has been used to analyze textile finishes. It is still the gold standard in that aU newly developed methods are judged by how well results from the new method compare to results from elemental analysis. Traditional wet chemical methods, such as Kjeldahl nitrogen analysis, continue to have a place in finish analysis. Elemental analysis can be qualitative or quantitative, depending on the need. 

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