Neurospora crassa mutants

Enzyme Systems. Carotenoid biosynthesis by crude cell-free preparations from Halobacterium cutirubrum 0-carotene), Phycomyces blakesleeanus mutants (/8-carotene), and a Neurospora crassa mutant (phytoene) has been demonstrated. Detailed studies of carotenogenic enzymes from tomato fruit... 

Figure 3 The reconstitution of aponitrate reductase from the Nit-1 Neurospora crassa mutant by MPT-containing extracts from the molybdenum and tungsten enzymes.

A sensitive and specific bioautographic method for the identification of choline and its derivatives, was developed by Lewin and Marcus, utilizing a Neurospora crassa mutant [5]. As little as 0.03 y of choline chloride is detectable. The applicability of the method for detection of choline derivatives in lipid hydrolyzate is discussed. A method for determining the rate of diffusion of compounds from paper chromatograms into bioautograph agar is described. 

This activity was found first in the Neurospora crassa mutant deficient in adenine, where the concentrations of ATP and other adenyl nucleotides were sharply reduced (Kulaev and Bobyk, 1971). 

L. V. Trilisenko, V. M. Vagabov and I. S. Kulaev (1980). The isolation and some peculiarities of Neurospora crassa mutant with deficiency in polyphosphate phosphohydrolase (in Russian). Mikrobiologiya, 49, 82-87. 

Metabolism of Palmitate Differs in Neurospora crassa Mutants with Impaired Fatty Acid Synthase. 

Figures 1 and 2 are schemes taken from Dalgliedi (S7) and Henderson (S8) which summarize the present views r ardmg the convermon of tryptophan to niacin. A great deal of evidence in support of the sdiemes has been obtained from isotope studies of vdiole cells and also from experiments with specific Neurospora crassa mutants.

The use of mutant 34486 of Neurospora crassa for the microbiological assay of ch oline has been described (8). A physiological method has also been used in which the ch oline is extracted after hydrolysis from a sample of biological material and acetylated. The acetylcholine is then assayed by a kymographic procedure, in which its effect in causing contraction of a piece of isolated rabbit intestine is measured (33). 

The molybdenum cofactor was liberated from D. gigas AOR, and under appropriate conditions was transferred quantitatively to nitrate reductase in extracts of Neurospora crassa nit-1 mutant) to yield active nitrate reductase 217). On the basis of molybdenum content, the activity observed for reconstitution with molybdenum cofactor of D. gigas was lower (25%) than the values observed for the procedure using extractable molybdenum cofactor of XO, used as reference. This result can now be put in the context of the difference in pterin present (MPT-XO and MCD-AOR) 218). 

Goldie, A.H. and Subden, R.E., The neutral carotenoids of wild-type and mutant strains of Neurospora crassa, Biochem. Genet. 10, 275, 1973. 

Purification and characterisation of galactose-induced pectinases from the exo-1 mutant strain of Neurospora crassa... 

DIMS was identified as the responsible gene for one of DNA hypomethylation mutants in Neurospora crassa (Tamaru and Selker, 2001). This is the first report that histone (H3K9) methylation regulates DNA methylation. Furthermore, it is reported that this regulation is mediated through the HPl recruitment to the tri-methylated H3K9 loci (Freitag et al, 2004), but it remains to be elucidated whether DNA methylation (DNA methyltransferase) is directly controlled (recruited) by HPl. 

Gardner GF, Feldman JF 1981 Temperature compensation of circadian periodicity in clock mutants of Neurospora crassa. Plant Physiol 68 1244-1248 Gorl M, Merrow M, Huttner B, Johnson J, Roenneberg T, Brunner M 2001 A PEST-hke element in FREQUENCY determines the length of the circadian period in Neurospora crassa. EMBO J 20 7074-7084... 

Neurospora crassa (wild type and 286-lOHSa mutant [1]) [1] <4> Penicillium chrysogenum (Q-176 [1]) [1]... 

Prior to 1941, mj/o-inositol was often determined by isolation, as such or as the hexaacetate.87 The isolated inositol was weighed, or was oxidized to carbon dioxide (which was measured in a gas buret).92 In 1941, Woolley98 published his microbiological method, in which the yeast Saccharomyces cerevisiae was used as a test organism. Soon afterward, there appeared an improved procedure, using Saccharomyces carlsbergensis,9i 96 and an additional method based on the discovery of an inositol-less mutant of the common bread-mold, Neurospora crassa.96-98 Plundreds of types of foods,... 

Fungi Mutation Neurospora crassa ad-3 system red adenine mutants Gene (forward) mutations and small deletions in ad3A and ad3B <2 mo M M M L... 

Figure 8.28 Changes in the content of PolyP fractions during growth of Neurospora crassa strains ad-6 (parent strain) and 30,19-3 (a leaky mutant in exopolyphosphatase), and a slime mutant devoid of the cell envelope (Trilisenko et al., 1980 1982a,b) (o) growth ( ) PolyP content in different fractions.

F. M. Harold (1960). Accumulation of inorganic polyphosphate in mutants of Neurospora crassa. Biochim. Biophys. Acta, 45, 172-188. 

I. S. Kulaev and H. Urbanek (1966). The dependence of 32P uptake by an adenine-delicient mutant of Neurospora crassa on the adenine content of the medium and on the presence of 8-azaadenine (in Russian). Dokl. Akad. Nauk SSSR, 169, 958-961. 

The assimilatory enzyme from the mold Neurospora crassa has been intensively studied for over two decades, particularly by Nason and his collaborators. Thus, Nason and Evans (39) identified FAD as a prosthetic group in the enzyme Nicholas, Nason, and McElroy (40) showed that molybdenum was required for the synthesis of nitrate reductase Nicholas and Nason (41) suggested its presence in the enzyme Garrett and Nason (42) showed that a b-type cytochrome (cytochrome 6557) co-purifies with this nitrate reductase and Nason et al. (11) suggested, from in vitro complementation experiments with nitrate reductaseless mutants, that the enzyme consists of at least two components required for activity. These workers have suggested that the electron transfer pathway is ... 

---