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Bioautographic methods

Physical methods, some of which can be applied in the on-line mode (Fig. 8) and physiological methods providing information concerning the effectivity of the separated substance can be used. A later volume will treat these bioautographic methods which can be subdivided into bioassays (off-line) and bioautogrammes (on-line). [Pg.36]

A sensitive and specific bioautographic method for the identification of choline and its derivatives, was developed by Lewin and Marcus, utilizing a Neurospora crassa mutant [5]. As little as 0.03 y of choline chloride is detectable. The applicability of the method for detection of choline derivatives in lipid hydrolyzate is discussed. A method for determining the rate of diffusion of compounds from paper chromatograms into bioautograph agar is described. [Pg.24]

The dilution and diffusion methods mentioned above are all well-known classical microbial assays. Only the bioautographic methods are suitable for direct combination of the detection techniques with any kind of chromatographic separation methods. This technique opens up new avenues to detect the effects of samples on microbes after separation of the compounds by opened sorbent-bed (e.g., TLC) chromatography. [Pg.283]

It must be noted that there are no sharp division lines between the different techniques. By the use of bioautographic methods great variety of assays can be carried out. [Pg.284]

With the bioautographic method described before (5) samples show elongated zones of inhibition, the confirmation of tetracyclines is done by HPLC. [Pg.473]

Some of the early reports on the chromatography of the tetracycline antibiotics prior to 1957 are of limited value. Fischbach and Levine (43) described a continuous ascending technique and Berti and Cima (44) reported an ascending method using aqueous sodium arsenite as the mobile solvent. Other authors(45,46) reported descending techniques and bioautographic means for locating the zones of activity. [Pg.125]

Methods useful to detect cephalosporins on chromatograms are short wave (254 nm) ultraviolet light, ninhydrin spray, sodium hydroxide-iodine-starch spray l, and bioautographs using Bacillus subtilis ATCC 6633 ... [Pg.337]

Rahalison, L., Hamburger, M., Hostettmann, K, Monod, M., andFrenk, E. (1991) A bioautographic agar overlay method for the detection of antifungal compounds from higher plants. Phytochem Anal. 2, 199-203. [Pg.244]

A ready-to-use kit (Merck, Germany), called Chrom Biodip Antibiotika, has recently become available. This was developed for special direct bioautographic detection. This kit contains a premixed culture medium, Bacillus subtilis spore suspension, nutrient medium, and MTT detection reagent. Generally, these methods require only heater and therefore it is possible to use this detection in a conventional chemical laboratory. The total determination time is 21 h. The main advantages of this test kit are (1) the test needs little laboratory time and cost, (2) the user does not need to maintain strains of bacteria for the test, and (3) the spore suspension can be used at any time. [Pg.285]

For biological/physiological detection, the separated compounds can be transferred to the biological system. Alternatively, bioautographic analyses, reprint methods, and enzymatic tests may also be applied. [Pg.836]

Using techniques previously described, Barath et al. classified the antibiotics from crude concentrates of fermentation media and from the mycelia of 50 strains of soil fungi. Utilizing the systematic PC methods with bioautographic techniques against Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and Candida pseudotropicalis, the antibiotics were grouped into a series of classes which permitted the authors to choose strains that produced antibiotics with specific activities. [Pg.4]

In an extension of these methods, Issaq et al. " classified 151 antibiotics exhibiting antitumor properties using TLC and bioautographic detection. Aszalos and Issaq similarly described TLC systems for classification and identification of various antibiotics. [Pg.5]


See other pages where Bioautographic methods is mentioned: [Pg.931]    [Pg.1505]    [Pg.137]    [Pg.257]    [Pg.228]    [Pg.2191]    [Pg.617]    [Pg.545]    [Pg.1433]    [Pg.3]    [Pg.14]    [Pg.21]    [Pg.82]    [Pg.931]    [Pg.1505]    [Pg.137]    [Pg.257]    [Pg.228]    [Pg.2191]    [Pg.617]    [Pg.545]    [Pg.1433]    [Pg.3]    [Pg.14]    [Pg.21]    [Pg.82]    [Pg.109]    [Pg.557]    [Pg.255]    [Pg.39]    [Pg.20]    [Pg.281]    [Pg.94]    [Pg.568]    [Pg.354]    [Pg.218]    [Pg.7]    [Pg.11]    [Pg.16]    [Pg.218]   
See also in sourсe #XX -- [ Pg.21 , Pg.257 ]

See also in sourсe #XX -- [ Pg.257 ]




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Bioautographs

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