Phycomyces blakesleeanus

Carotene Yellow-orange Phycomyces blakesleeanus (fungus) RP... 

The core of the iron storage protein ferritin consists of a hydrated ferric oxide-phosphate complex. Various models have been proposed which feature Fe111 06 oct., Fe111 O4 tet. or Fe111 O4 tet. Fe111 06 oct. complexing the first listed is preferred by Gray (99) on the basis of the electronic absorption spectrum. The protein very closely related to ferritin which occurs in the mold Phycomyces blakesleeanus contains... 

The incorporation of " C-labelled neurosporene (138), lycopene, and y-carotene (141) into /3-carotene by cell extracts of Phycomyces blakesleeanus mutants has been demonstrated.Addition of unlabelled lycopene or /3-zeacarotene (140) caused approximately equal reduction of the incorpsration of [ C]neurosporene into /3-carotene, indicating that the alternative routes of Scheme 3 are of equivalent importance. The absolute configuration of C-6 of natural /3,y-carotene (55) is opposite to that of all C40 carotenoids with an e-ring end-group. " Opposite foldings of the aliphatic precursor are therefore required for cyclization to produce the y- and e-end-groups. 

Enzyme Systems. Carotenoid biosynthesis by crude cell-free preparations from Halobacterium cutirubrum 0-carotene), Phycomyces blakesleeanus mutants (/8-carotene), and a Neurospora crassa mutant (phytoene) has been demonstrated. Detailed studies of carotenogenic enzymes from tomato fruit... 

Further genetic studies have provided additional information about the regulation of carotenogenesis in Phycomyces blakesleeanus. ... 

Addition of vitamin A to a medium supporting the growth of Phycomyces blakesleeanus led to a large increase in the j8-carotene content of the wild strain,362 although the additive was not itself converted into product. Vitamin A is possibly sufficiently similar in structure to the natural regulators of carotenogenesis (possibly trisporic acids) as to be able to simulate their effect. 

It is under unsaturated conditions that the structure of the AM fungal mycelium becomes of particular interest. The hydraulic conductivity of the entire hypha (Lp) of fungal hyphae of Phycomyces blakesleeanus was measured at 4.6 x 10 cm (s MPa) (Cosgrove et al., 1987) but that... 

Cosgrove, D. 1., Ortega, 1. K. E. Shropshire, W., Jr. (1987). Pressure probe study of the water relations of Phycomyces blakesleeanus sporangiophores. Biophysical Journal, 51, 413-24. 

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