Neuro-2a

Tf-containing PEG-shielded polyplexes have also been applied for systemic tumor-targeted delivery of siRNA [106-108]. Systemic treatment of Neuro 2A tumorbearing mice using Tf-PEG-shielded crosslinked oligoethylenimines for delivery of siRNA against Ras-related nuclear protein (Ran) led to >80% reduced Ran protein expression, associated with tumor apoptosis and reduced tumor growth [108]. 

Inokoshi J, Katagiri M, Arima S, Tanaka H, Hayashi M, et al. 1999. Neuronal differentiation of Neuro 2a cells by inhibitors of cell cycle progression, trichostatin A and butyr-olactone I. Biochem Biophys Res Commun 256 372-376. 

Table 7. Combined chemotherapy and photodynamic therapy of A/J mice bearing Neuro 2A neuroblastoma tumors using HPMA copolymer conjugates (from [42])...

IC5Q in Neuro 2a cell line, McLean and Edwards (University of Liverpool). b Standard in vitro antimalarial screening data, D-6 clone of P. falciparum. 

The germanium derivative of furfural oxime had significant activity on HT-1080, MG-22A and B16 cells, but not on Neuro 2A. The activity of carbon and silicon analogues was lower in all cell lines (Table 18)139. 

Jamaicamides A, B, and G exhibited cytotoxidty to both the H-460 human lung carcinoma and neuro-2a mouse neuroblastoma cell lines. The LG50S were approximately 15 p,M for all three compounds to both cell lines. All three compounds also exhibited sodium charmel-blocking activity at 5 p,M concentration. In the goldfish toxicity assay, a system that has been useful for the detedion of neurotoxic activity in cmde extrads as well as purified compounds, jamaicamide B was the most active (100 % lethality at 5 ppm after 90 min), followed by jamaicamide G (100 % lethality at... 

Chen, Y. et al. Development of an ion-pair HPLC method for investigation of energy charge changes in cerebral ischemia of mice and hypoxia of Neuro-2a cell line. Biomed. Chromatogr. 2007, 21, 628-634. 

We have also determined the cytotoxicities of natural PA and the synthetic analognes 3-6 in the mouse neuroblastoma cells, Nemo-2a. Due to the limitation of availability of the componnds, the cytotoxicities were tested only at 12 pM by counting the viable cells treated with toxins for 24 h. We observed that 5 and 6 elicited more than 90% death responses, whereas PA elicited only 20% death responses, and 3 and 4 did not show cytotoxicity at this level, snggesting this cytotoxicity not to be comparable to that in mice. We proved that 5 indnced apoptosis in Neuro-2a at 12 pM within... 

Potential cytotoxic activity of synthesized compounds 1-7 was tested in vitro on three monolayer tumor cell lines MG-22A (mouse hepatoma), HT-1080 (human fibrosarcoma), and Neuro 2A (mouse neuroblastoma). Concentrations providing 50% of tumor death effect (IC50) were... 

Two new sesterterpenes, 6-epi-ophiobolin G 49 and 6-epi-ophiobolin N 50, were isolated from the extraets of the fungus, Emericella variecolor GFIO obtained from marine sediment/ These eompounds showed cytotoxicity against the neuroblastoma cell line, Neuro 2A. 

Shimalactone A 79, a novel polyketide having bicyclo[4.2.0]octadiene and oxabicyclo[2.2.1]heptane units, was isolated from a cultured marine fungus of Emericella variecolor GFIO. Shimalactone A 79 induced neuritogenesis at 10 mg/mL against neuroblastoma Neuro 2A cells. At higher concentration of 20 mg/mL, it showed cytotoxicity against the same cell line. 

A Papua New Guinea eolleetion of the marine cyanobacterium Lyngbya majuscula yielded two new toxic natural products, hermitamides A 89 and B 90. Hermitamides 89 and 90 exhibited LD50 values of 5 pM and 18 pM, respectively, in the brine shrimp (Artemia salina) bioactivity experiment, and IC50 values of 2.2 pM and 5.5 pM to Neuro-2a neuroblastoma cells in tissue culture. Hermitamide A was mildly ichthyotoxic to goldfish, with an LD50 value of 19 pM, while hermitamide B was inactive at 25 pM. 

Katagiri, M., Hayashi, M., Matsuzaki, K., et al. (1995). The neuritogenesis inducer lactacystin arrests cell cycle at both GO/Gl and G2 phases in neuro 2a cells. JAntibiot., 48, 344-346. 

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