Human HT-1080 fibrosarcoma

Berberine chloride was demonstrated to possess significant cytotoxic activity against cultured P-388 (murine lymphocytic leukemia) cells, in addition to three human cancer cell lines BC1 (breast cancer) - EDJ0 2.7 pM) HT-1080 (fibrosarcoma) - ED50 2.4 pM) and KB (nasopharyngeal carcinoma) - EDJ0 8.4 pM)[336]. 

Synthetic effort is going on toward the unique diarylheptanoid blepharocalyxin D, 38, which has shown considerable in vitro antiproliferative activity against human fibrosarcoma HT-1080 cells, although this postulated stmcture for blepharocalyxin D is yet to be confirmed by synthesis <2003TL6351>. 

A number of 1,3-selenazole and 1,3-selenazine derivatives have been reported as antiproliferative agents. The most active against human fibrosarcoma HT-1080 cells were 1,3-selenazines (166) [269], Further, certain 2-phosphonoalkylben-zisoselenazol-3(2//)-ones (167) were synthesized and their potent activity against human carcinoma cells in vitro was reported [164],... 

Table 2 Influence of a silacycloalkyl substituent on cytotoxicity (LC50, / gml CV ) against human fibrosarcoma HT-1080 (08ISOC112)...

Potential cytotoxic activity of synthesized compounds 1-7 was tested in vitro on three monolayer tumor cell lines MG-22A (mouse hepatoma), HT-1080 (human fibrosarcoma), and Neuro 2A (mouse neuroblastoma). Concentrations providing 50% of tumor death effect (IC50) were... 

Sato T, Koike L, Miyata Y, Hirata M, Mimaki Y, Sashida Y, Yano M, Ito A. Inhibition of activator protein-1 binding activity and phosphatidylinositol 3-kinase pathway by nobiletin, a polymethoxy flavonoid, results in augmentation of tissue inhibitor of metaUoproteinases-1 production and suppression of production of matrix metalloproteinases-1 and -9 in human fibrosarcoma HT-1080 cells. Cancer Res 2002 62 1025-1029. 

Nagase H, Sasaki K, Kito H, Haga A, Sato T (1998) Inhibitory effect of delphinidin from Solanum melongena on human fibrosarcoma HT-1080 invasiveness in vitro. Planta Med 64 216-219 Nam S, Smith DM, Dou QP (2001) Tannic acid potently inhibits tumor cell proteasome activity, increases p27 and Bax expression, and induces G1 airest and apoptosis. Cancer Epidemiol Biomarkers Prev 10 1083-1088... 

In an in vitro model, tumour metastases (B16BL6 cells) and invasion human fibrosarcoma HT-1080) were inhibited by repeated addition of 300 iiM 2-0-phosphorylated ascorbate (Nagao et al. 2000). Intracellular vitamin C increased and both hydroxyl and ascorbyl radicals decreased as quantified by electron spin resonance spectroscopy. 

In a mouse model of liver metastasis, B717 siRNA complexed with LIC-101 administered by tail vein injection almost completely suppressed the growth of HT-1080 cells, a human fibrosarcoma cell line, at a dose of 1 mg/kg (Fig. 4, left). No effect was produced by naked B717 or LIC-101 alone (Fig. 4, right). In contrast, a dose of 4.2 mg/kg was required for the same effect with antisense DNA (Fig. 4, left) [71]. We suspect that the effect observed at this high dose was nonspecific. 

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