Myelin Basic Protein MBP

Myelin is a major component of neural tissue lipids and contains unique proteins such as myelin basic protein. Changes in cellular lipid composition can reflect demy-elination, where these changes may consist of a decrease in myelin or protein/lipid with an increase in water content accompanied by the appearance of cholesterol esters (Norton 1984). Myelin basic protein content or synthesis may be determined to quantify myelination in the brain or reactive synthesis secondary to a toxic insult (Conti et al. 1996 Hamano et al. 1996). 

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The conformation of bovine myelin basic protein (MBP) in AOT/isooctane/water reversed micellar systems was studied by Waks et al. 67). This MBP is an extrinsic water soluble protein which attains an extended conformation in aqueous solution 68 but is more density packed at the membrane surface. The solubilization of MBP in the AOT reversed micelles depends on the water/AOT-ratio w0 68). The maximum of solubilization was observed at a w0-value as low as 5.56. The same value was obtained for another major protein component of myelin, the Folch-Pi proteolipid 69). According to fluorescence emission spectra of MBP, accessibility of the single tryptophane residue seems to be decreased in AOT reversed micelles. From CD-spectra one can conclude that there is a higher conformational rigidity in reversed micelles and a more ordered aqueous environment. 

Shiverer mouse, myelin-deficient mouse AR Myelin basic protein (MBP) Deletion or inversion of several MBP exons very little functional MBP expressed severe CNS hypomyelination and failure of compaction of major dense line see text 1,10,43... 

The intramuscular injection of naked pDNA has also been evaluated for therapy of EAE, a mouse model of multiple sclerosis. The disease is induced in mice by s.c. injection of myelin basic protein (MBP) or proteolipid protein (PLP). In a recent study, mice were injected i.m. with pDNA encoding either IL-4/IgG or TGF / 48 hours prior to each MBP injection (on days zero and seven) (Piccirillo and Prud -homme, 1999). The disease severity was 70% lower in the IL-4/IgG or TGF / -treated mice and the mean clinical scores were significantly reduced in both groups. In addition, the IL-4 and TGF -treated mice had significantly reduced CNS inflammation, reduced T cell proliferative responses to MBP and low levels of the Th 1 cytokines IL-12 and IFN7. Thus, the pDNA therapy appeared to shift the mice to a Th2 response, which was therapeutic and reduced the severity of the disease. 

Myelin basic protein (MBP) Oligodendrocytes, glioma cells [139, 140]... 

Myelin basic proteins (MBP). Basic proteins are abundant both in CNS and PNS myelin, where they are associated with negatively charged lipids. They are assumed to be involved in myelin compaction on the cytoplasmic side of the membrane bilayer. A spontaneous MBP mutant, the shiverer mouse, is devoid of the major dense line of myelin in the CNS (Dupouey et al., 1979). Another such mutant, the Long Evans Shaker (LES) rat, shows major changes in spinal cord white matter, with dispersed labeling of Kvl.l and Kvl.2 K+ channel subunits as well as of Caspr, a molecule normally confined to paranodes along LES rat spinal axons (Eftekharpour et al, 2005). 

Recent evidence obtained via magnetic resonance imaging and spectroscopy techniques supports the view that the normal-appearing white matter (NAWM) in the MS brain is altered. Several biochemical changes in NAWM have been determined. These include the cationicity of myelin basic protein (MBP) as a... 

Role of MBP MBP is one of the major proteins of the CNS, and it constitute about 25-30% of the total protein. The 18.5kDa isoform of myelin basic protein (MBP) is the exemplar of the family, being most abundant in adult myelin, and thus the most-studied. Shiverer mutation of MBP gene results in the absence of MBP proteins and morphological analysis of the CNS reveals an almost total lack of myelin in the brain, and also the existing myelin is abnormal, presenting no major dense line. Therefore, MBP is necessary for the formation of the major dense line in the CNS myelin (Readhead and Hood, 1990). 

Table 7 lists S-100-positive tumors and their positivity with additional markers. Myelin basic protein (MBP) (30) is a single-chain polypeptide associated with the central and peripheral nervous system. Glial fibrillary acidic protein (GFAP) and neurofilament proteins (NFP) are intermediate filaments associated with glial cells and neural cells, respectively. HMB-45 is a mouse monoclonal antibody that reacts with an antigen present in premelanosomes. It is highly sensitive and specific for melanomas and certain nevi (junctional, congenital, blue nevi). EMA has been discussed in Section 3.1.3.1. 

The myelin formation is most often studied using microscopic analysis (including electron microscopy) of the immunocytochem-istry for specific proteins, such as myelin basic protein (MBP), protein zero, myelin associated glycoprotein (MAG), and peripheral myelin protein 22 [107, 108]. The same markers can also be evaluated by other protein assays (e.g., enzyme-linked immunosorbent assay (ELISA) and western blot) or at mRNA level using for example PCR or microarray assays. 

One example of Na channel plasticity in central pathways subsequent to a non-ion channel gene deletion is revealed by analysis of the mutant mouse shiverer, which bears a major disruption of the myelin basic protein gene, leading to absence of the glial-specific intracellular membrane protein myelin basic protein (MBP) (Roach et al 1985). Lack of MBP prevents developmental formation of the major dense line essential for the compaction of glial wrappings that form the mature myelin sheath. As a result, large calibre axons in the shiverer brain are... 

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