Protein zero

Filbin, M. T., D Urso, D., Zhang, K., Wong, M., Doyle, J. P. and Colman, D. R. Protein zero of peripheral nerve myelin adhesion properties and functional models. Adv. Mol. Cell. Biol. 16 159-192,1996. 

Lemke, G., Lamar, E. and Patterson, J. Isolation and analysis of the gene encoding peripheral myelin-protein zero. Neuron 1 73-83,1988. 

D Urso, D., Brophy, P. J., Staugaitis, S. M. etal. Protein zero of peripheral myelin biosynthesis, membrane insertion, and evidence for homotypic interactions. Neuron 4 449 60,1990. 

Set the sensitivity in the UV monitor control unit to a suitable value that will enable peak detection at a given concentration of protein Zero the baseline or chart recorder. 

Net protein ratio (NPR) is used to correct PER values for the amount of protein required for cell maintenance. NPR is often run in conjunction with a PER. The experiment requires that one additional set of animals be added as a treatment group. This group of animals is fed a basal diet with no protein (zero protein or basal diet). Results from RNPR are similar to net protein utilization (NPU) and biological value (BV methods, see Alternate Protocol 4). A 2-week RNPR is thought to be the most appropriate rat test for routine assessment of protein quality. 

Mpz, myelin Protein Zero Peripheral myelin component, transmembrane cell adhesion KO, Tg... 

Wrabetz, L., D Antonio, M., Pennuto, M., Dati, G., Tinelli, E., Fratta, P., et al. (2006) Different intracellular pathomechanisms produce diverse Myelin Protein Zero neuropathies in transgenic mice. J Neurosci 26, 2358-2368. 

Water-soluble carbodiimide for die modification of carboxyl groups in proteins zero-length crosslinking proteins activation of carboxyl groups for amidation reactions, as for the coupling of amino-nucleotides to mattices for DNA microarrays. 

Spiryda LB (1998) Myelirr protein zero and membrane adhesion. J Neurosci Res 15 137—146. 

Mardni R, Zielasek J, Toyka KV, Giese KP, Schachner M (1995) Protein zero (PO)-deficient mice show myelin degeneradon in peripheral nerves char acteiisdc of inherited human neuropadiies. Nat Genet 11 281-286. 

The myelin formation is most often studied using microscopic analysis (including electron microscopy) of the immunocytochem-istry for specific proteins, such as myelin basic protein (MBP), protein zero, myelin associated glycoprotein (MAG), and peripheral myelin protein 22 [107, 108]. The same markers can also be evaluated by other protein assays (e.g., enzyme-linked immunosorbent assay (ELISA) and western blot) or at mRNA level using for example PCR or microarray assays. 

Xie B, Luo X, Zhao C, Priest CM, Chan S-Y, O Connor PB, Kirschner DA, Costello CE. Molecular characterization of myelin protein zero in Xenopus laevis peripheral nerve equilibrium between non-covalently associated dimer and monomer. Int J Mass Spectrom 2007 268 304-315. 

Martini, R. Zielasek, J. Toyka, K.. V. Giese, K. P. Schachner, M. Protein zero(PO)-deficient mice show myelin degeneration in peripheral nerves characteristic of inherited human neuropathies. Nat. Genet. 1995, II 281-286. 

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