Mononuclear cell fraction

Enrichment of rare cancer cells from peripheral blood samples is an application that typically requires density gradient centrifugation as a first step. Application of this technique addresses two objectives depletion of erythrocytes and depletion of polymorphonuclear cells. It is expected that cancer cells undergo sedimentation with the mononuclear cell fraction because of their similar density. However, some studies have found that cancer cells are also lost in the polymorphonuclear fraction or the erythrocyte fraction (8,9). Optimization of the density gradient sedimentation step is an important issue in such an application, because it will determine the recovery of rare cells from blood and affect the chances of their detection by immunochemical means. 

Transfer the mononuclear cell fraction to a sterile 50-mL screw-cap centrifuge tube containing 35 mL of PBS P/S. 

Patterson J, Moore CH, Palser E, Hearn JC, Dumitru D, Harper HA, Rich IN. Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units. J Transl Med. 2015 13 94. 

Preparation of mononuclear cell fraction (MNC) from bone marrow (BM) harvests using a COBE 2991 cell processor... 

Natural-killer cell enhancement. Polysaccharide fraction of the rhizome, administered intragastrically to female mice at a dose of 1 g/kg, produced weak activity vs mononuclear cell incubated with YAG-1 cells ° 5... 

Abbreviations BM-MNC, bone marrow mononuclear cell CABO, coronary artery bypass grafting CPC, circulating progenitor cells LV. left ventricle LVED. left ventricular end-diastolic diameter LVEDV, left ventricular end-diastolic volume LVEF, left ventricular ejection fraction NYHA, New York Heart Association SKMB, skeletal myoblast. 

Abbreviations AMI. acute myocardial infarction BM-MNC. bone marrow mononuclear cell LVEF, left ventricular ejection fraction SEM. standard error of mean TOPCARE-AMI, transplantation of progenitor cells and regeneration enhancement in acute myocardial infarction MX. mesenchymal stem cell. 

Torre, P.M. and Oliver, S.P. 1989a. Inhibition of bovine peripheral blood mononuclear cell blastogenesis by fractionated mammary secretion. Comp. Biochem. Physiol. B 92, 157-165. 

A partially purified fraction of astragalus coadministered to mice with the immune suppressant cyclophosphamide after transplantation of mononuclear cells resulted in transplant rejection, indicating a reversal of cyclophosphamide action (Chuetal. 1988b). 

Peripheral mononuclear cells (PBMC) were isolated from heparinized blood by standard gradient centrifugation (B yum, 1968). T-enriched cell fractions were obtained by resetting PBMC with 2-aminoethylisotbiouronium bromide-treated sheep red blood cells (Madsen and Jobnsen, 1979). Cell suspensions were adjusted to a concentration of 5 x 10 cells/ml and sonicated at 23 kc/sec. 

INFy) release from mouse spleen cells, and the maximum increase was approximately three-fold for IL-2, 9.5-fold for TNFa and 1.8-fold for INFy, respectively [24]. Furthermore, a protein fraction of AGE has been shown to significantly augment Con A-induced IL-2, TNFa and INFy release, and IL-2-induced TNFa and INFy release from human peripheral lymphocytes [23]. Aqueous garlic extract was also reported to increase in vitro IL-1 and 2 production in human peripheral blood mononuclear cells [25]. 

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