Microorganism detection

Pindzola BA, Nguyen AT, Reppy MA (2006) Antibody-functionalized polydiacetylene coatings on nanoporous membranes for microorganism detection. Chem Commun 8 906-908... 

N-Compounds Microorganisms Detection limit (mg I T Response time (min) Ref... 

Analyte Microorganisms Detection limit [mgl ] Standard deviation (%) Response time (min) Stability (d) Specificity interferences Reference... 

Analyte Microorganisms Detection Standard Response stability Specificity interferences Reference... 

The main objective of validation of an analytical procedure is to demonstrate that the procedure is suitable for its intended purpose. The procedures presented in this SOP provide basic guidelines for the validation of methods for microbiological assay, estimation of the number of microorganisms, detection of indicators of objectionable microorganisms, validation of preservative efficacy testing, and validation of the sterility testing and endotoxin test (LAL test). 

Zehr, J. P., Mellon, M. T., and Zani, S. (1998). New nitrogen-fixing microorganisms detected in oligotrophic oceans by the amplification of nitrogenase (nifH) genes. Appl. Environ. Microbiol. 64, 3444-3450. 

Substrate Microorganism Detection mode Measuring range (mmol/1)1 References... 

Application areas for DNA biosensors include the detection of chemically induced DNA damage by toxic compounds and the detection of pathogenic microorganisms through the hybridization of species-specific DNA sequences. However, this section will only concentrate on the development of DNA-based sensors for toxic compound detection as microorganism detection is not covered in this chapter. 

Hayden, O. Dicker , F.L. Selective microorganism detection with cell surface imprinted polymers. Adv. Mat. 2001,13 (19), 148(U1483. 

Major Applications Display device, optical sensors, combustion gas detection system, inks, correction fluid,diapers,n toothpaste, identifying fresh and stale rice, food storage,i" determine glucose in dialysis solution, monitor metabolic activity of microorganisms, detect bacterial infection, psychoactive drugs, dental impression materially Safety/Toxicity No data available... 

Both the Institute of Petroleum and the ASTM acknowledge the lack of generally accepted criteria for what would be considered significant microbial contamination. The Institute of Petroleum indicates that microbial counts in the water bottom of 10 -10 CFU/L bacteria, 10 -10 CFU/L yeasts, 10 CFU/L molds and 10 CFU/L SRB, either alone or in combination, are considered to represent microbial contamination (Institute of Petroleum, 1996). The ASTM suggests that counts of 10 CFU/ml can be, in many cases, accepted in the water bottom but any microorganisms detected in the fuel should suggest the need for corrective measures (ASTM, 2001). The ASTM also cautions that microbial counts should be routinely monitored and if problems with fuel chemistry, performance or physical appearance are also noted then corrective action should be considered. 

Yoshida, S. Soejima, T. Microorganism detection method by nuclear staining and flow cytometry, and microorganism detection kit. PCX Int. Appl. WO 2007010641, 2007 Chem. Abstr. 2007,146, 138259. 

Biological Applications Analysis of microorganisms detecting anti-bacterial agent, y-hydroxybutyric acid (GHB) treating cancer, pain °... 

Abe, F. Horikoshi, H. The microorganism detection by the flow cytometry method. Jpn. Kokai Tokkyo Koho JP 2000069995,2000. 

Table 6 Potassium and calcium mass fractions (g g ) of different microorganisms. Detection limits of K and Ca are 100 and 80 gg g , respectively. Reproduced from Reference [28] with permission of the author and publisher.

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